1. Temporal control of xyloglucan self-assembly into layered structures by radiation-induced degradation.
- Author
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Todaro S, Sabatino MA, Mangione MR, Picone P, Di Giacinto ML, Bulone D, and Dispenza C
- Subjects
- Cell Line, Tumor, Cell Survival drug effects, Glucans chemical synthesis, Glucans radiation effects, Humans, Hydrogels chemistry, Materials Testing, Neuroblastoma pathology, Polymerization radiation effects, Shear Strength, Spectroscopy, Fourier Transform Infrared, Temperature, Time Factors, Viscosity, Xylans chemical synthesis, Xylans radiation effects, Biopolymers chemistry, Biopolymers pharmacokinetics, Biopolymers radiation effects, Gamma Rays, Glucans chemistry, Glucans pharmacokinetics, Hydrogels chemical synthesis, Hydrogels pharmacokinetics, Xylans chemistry, Xylans pharmacokinetics
- Abstract
Partially degalactosylated xyloglucan from tamarind seeds (Deg-XG) is a very appealing biopolymer for the production of in situ gelling systems at physiological temperature. In this work, we observe that the morphology of hydrogels evolves towards high degrees of structural organization with time, yielding to dense stacks of thin membranes within 24h of incubation at 37°C. We also explore the possibility offered by gamma irradiation of controlling the time scale of this phenomenon, the final morphology and mechanical properties of the system. Structural and molecular modifications of Deg-XG with dose are investigated by FTIR, dynamic light scattering (DLS) and rotational viscosimetry. The impact on gelation ability and gel strength is studied by rheological analysis. The morphology evolution is investigated by SEM analysis, and absence of cytotoxicity verified by MTS assay and optical microscopy of neuroblastoma cells., (Copyright © 2016 Elsevier Ltd. All rights reserved.)
- Published
- 2016
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