1. Cell Recovery of Hydrogel-Encapsulated Cells for Molecular Analysis.
- Author
-
Peerani E, Candido JB, and Loessner D
- Subjects
- Cell Line, Tumor, Cell Proliferation, Cell Survival, Culture Media chemistry, Flow Cytometry methods, Humans, Microscopy, Confocal, Microscopy, Fluorescence, Spheroids, Cellular, Cell Culture Techniques methods, Cell Encapsulation methods, Hydrogels
- Abstract
Tissue engineering technologies have produced controllable and reproducible three-dimensional (3D) models that mimic the architecture and complexity of native tissues. In particular cell biology-based research is driven by the development of cell culture platforms and techniques that allow the analysis of cells cultured in 3D. Here we summarize several easy-to-follow methods for the characterization of cells that have been encapsulated and grown in hydrogels to measure their cell viability, metabolic activity, and mechanical properties of cell-containing hydrogels. We also describe an enzymatic approach for the digestion of cell-containing hydrogels and cell recovery thereby maintaining high cell viability for subsequent analysis.
- Published
- 2019
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