1. H 2 S confers colonoprotection against TNBS-induced colitis by HO-1 upregulation in rats.
- Author
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Kupai K, Almási N, Kósa M, Nemcsók J, Murlasits Z, Török S, Al-Awar A, Baráth Z, Pósa A, and Varga C
- Subjects
- Animals, Anti-Inflammatory Agents pharmacology, Colitis metabolism, Colon drug effects, Colon metabolism, Inflammation chemically induced, Inflammation drug therapy, Inflammation metabolism, Male, Models, Animal, Rats, Rats, Wistar, Colitis chemically induced, Colitis drug therapy, Heme Oxygenase (Decyclizing) metabolism, Hydrogen Sulfide pharmacology, Trinitrobenzenesulfonic Acid pharmacology, Up-Regulation drug effects
- Abstract
Hydrogen sulfide (H
2 S) is an endogenous mediator that contributes to many important physiological processes including vasodilation and vascular smooth muscle relaxation; in turn, preventing tissue damage and reducing inflammation. Heme oxygenase (HO) enzymes, of which HO-1 is inducible by harmful stimuli, were found to regulate intestinal inflammation in experimental animal models of colitis. We aimed to investigate the protective effects of H2 S against 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis in rats, and whether HO enzyme system is involved in the H2 S-induced colonic cytoprotection. Male Wistar rats were treated with TNBS to induce colitis, and H2 S donor (Lawesson's reagent) was prepared two times/day at different concentrations, and delivered per os (from day 1 to day 3). Our results suggest that daily treatment (2 times/day) with H2 S donor, could significantly decrease the extent of colonic inflammation compared to vehicle treatment, and the most effective daily dose of H2 S donor against inflammation was 18.75 µM/kg/day. Per os administration of H2 S donor increased the colonic HO enzyme activity; on the contrary, the protective effect of H2 S was abolished by the co-treatment with HO inhibitor. Our findings suggest that H2 S confers colonoprotection, probably by modulation of anti-inflammatory parameters and HO enzyme activity.- Published
- 2018
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