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Methyl jasmonate (MeJA), a vital cellular regulator, mediates diverse developmental processes and defense responses against environmental stresse. Recently, a novel gasotransmitter, hydrogen sulfide (H2S), was found to have similar functions, but the interactions between H2S and MeJA in the acquisition of cadmium (Cd) tolerance have not been reported. Treating foxtail millet with 1 microM MeJA not only enhanced Cd tolerance and alleviated growth inhibitions but also decreased the contents of hydrogen peroxide, malondialdehyde and Cd in seedlings under 200 microM of Cd stress. Exogenous application of MeJA inhibited the transcript levels of the Natural Resistance-Associated Macrophage Protein (NRAMP1 and NRAMP6) and intensified Cd-induced expression of the homeostasis-related genes (MTP1, MTP12, CAX2 and ZIP4, besides HMA3). In addition, treatment with MeJA induced the production of endogenous H2S. Fumigation with sodium hydrosulfide (H2S donor) significantly enhanced MeJA-induced Cd tolerance, but this ability was weakened when H2S biosynthesis was inhibited with hydroxylamine. These results suggest that pretreatment with MeJA alleviated Cd stress and that this improvement was mediated by H2S in foxtail millet.

Hydrogen sulfide (H 2 S) and some functional amino acids in crops have been involved in the defense system against heavy-metal pollution. Here we report the relationships and functions of H 2 S and proline to cadmium (Cd) stress. Sodium hydrosulfide (NaHS) pretreatment decreased the electrolytic leakage and the malondialdehyde and hydrogen peroxide contents while enhancing photosynthesis in Cd-treated seedlings. Furthermore, pretreatment with NaHS markedly exacerbated Cd-induced alterations in proline content, the activities of proline-5-carboxylate reductase (P5CR) and proline dehydrogenase (PDH), and the transcript levels of P5CR and PDH. When endogenous H 2 S was scavenged or inhibited by various H 2 S modulators, the Cd-induced increase in endogenous proline was weakened. Combined pretreatment with H 2 S and proline was moderately higher in the Cd-stressed growth status, stomata movements and oxidative damage of seedlings compared to a single treatment with H 2 S or proline. These results suggest that H 2 S and proline cooperate to alleviate Cd-damage in foxtail millet., (Copyright © 2016 Elsevier Masson SAS. All rights reserved.)

Hydrogen sulfide (H(2)S) plays a crucial role in the regulation of stomatal closure in plant response to drought stress, and l-cysteine desulfhydrase (LCD) has been identified as being mainly responsible for the degradation of cysteine to generate H(2)S. In view of the similar roles to abscisic acid (ABA), in this study, the lcd, aba3 and abi1 mutants were studied to investigate the close inter-relationship between H(2)S and ABA. The lcd mutant showed enlarged stomatal aperture and more sensitivity to drought stress than wild-type plants. Expression of Ca(2+) channel and outward-rectifying K(+) channel coding genes decreased in the lcd mutant, and conversely, expression of inward-rectifying K(+) increased. The stomatal aperture of aba3 and abi1 mutants decreased after treatment with NaHS (a H(2)S donor), but stomatal closure in responses to ABA was impaired in the lcd mutant. The expression of LCD and H(2)S production rate decreased in both the aba3 and abi1 mutants. Transcriptional expression of ABA receptor candidates was upregulated in the lcd mutant and decreased with NaHS treatment. The above results suggested that H(2)S may be an important link in stomatal regulation by ABA via ion channels; H(2)S affected the expression of ABA receptor candidates; and ABA also influenced H(2)S production. Thus, H(2)S interacted with ABA in the stomatal regulation responsible for drought stress in Arabidopsis., (Copyright © 2012 Elsevier Masson SAS. All rights reserved.)