10 results on '"Sommer, Natascha"'
Search Results
2. Cytochrome P450 epoxygenase‐derived 5,6‐epoxyeicosatrienoic acid relaxes pulmonary arteries in normoxia but promotes sustained pulmonary vasoconstriction in hypoxia.
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Strielkov, Ievgen, Krause, Nicole C., Knoepp, Fenja, Alebrahimdehkordi, Nasim, Pak, Oleg, Garcia, Claudia, Ghofrani, Hossein A., Schermuly, Ralph T., Seeger, Werner, Grimminger, Friedrich, Sommer, Natascha, and Weissmann, Norbert
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CYTOCHROME P-450 ,PULMONARY artery ,VASOCONSTRICTION ,EPOXIDE hydrolase ,HYPOXEMIA ,OXYGENASES - Abstract
Aims: The aim of the study was to investigate the role of cytochrome P450 (CYP) epoxygenase‐derived epoxyeicosatrienoic acids (EETs) in sustained hypoxic pulmonary vasoconstriction (HPV). Methods: Vasomotor responses of isolated mouse intrapulmonary arteries (IPAs) were assessed using wire myography. Key findings were verified by haemodynamic measurements in isolated perfused and ventilated mouse lungs. Results: Pharmacological inhibition of EET synthesis with MS‐PPOH, application of the EET antagonist 14,15‐EEZE or deficiency of CYP2J isoforms suppressed sustained HPV. In contrast, knockdown of EET‐degrading soluble epoxide hydrolase or its inhibition with TPPU augmented sustained HPV almost twofold. All EET regioisomers elicited relaxation in IPAs pre‐contracted with thromboxane mimetic U46619. However, in the presence of KCl‐induced depolarization, 5,6‐EET caused biphasic contraction in IPAs and elevation of pulmonary vascular tone in isolated lungs, whereas other regioisomers had no effect. In patch‐clamp experiments, hypoxia elicited depolarization in pulmonary artery smooth muscle cells (PASMCs), and 5,6‐EET evoked inward whole cell currents in PASMCs depolarized to the hypoxic level, but not at their resting membrane potential. Conclusions: The EET pathway substantially contributes to sustained HPV in mouse pulmonary arteries. 5,6‐EET specifically appears to be involved in HPV, as it is the only EET regioisomer able to elicit not only relaxation, but also sustained contraction in these vessels. 5,6‐EET‐induced pulmonary vasoconstriction is enabled by PASMC depolarization, which occurs in hypoxia. The discovery of the dual role of 5,6‐EET in the regulation of pulmonary vascular tone may provide a basis for the development of novel therapeutic strategies for treatment of HPV‐related diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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3. Effects of carbon monoxide-releasing molecules on pulmonary vasoreactivity in isolated perfused lungs.
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Pak, Oleg, Bakr, Adel G., Gierhardt, Mareike, Albus, Julia, Strielkov, Ievgen, Kroschel, Florian, Hoeres, Timm, Hecker, Matthias, Ghofrani, Hossein A., Seeger, Werner, Weissmann, Norbert, and Sommer, Natascha
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PHYSIOLOGICAL effects of carbon monoxide ,PULMONARY blood vessels ,HYPOXEMIA ,CYTOCHROME P-450 ,VASOCONSTRICTION - Abstract
In addition to its renowned poisonous effects, carbon monoxide (CO) is being recognized for its beneficial actions on inflammatory and vasoregulatory pathways, particularly when applied at low concentrations via CO-releasing molecules (CO-RMs). In the lung, CO gas and CO-RMs are suggested to decrease pulmonary vascular tone and hypoxic pulmonary vasoconstriction (HPV). However, the direct effect of CO-RMs on the pulmonary vasoreactivity in isolated lungs has not yet been investigated. We assessed the effect of CORM-2 and CORM-3 on the pulmonary vasculature during normoxia and acute hypoxia (1% oxygen for 10 min) in isolated ventilated and perfused mouse lungs. The effects were compared with those of inhaled CO gas (10%). The interaction of CORM-2 or CO with cytochrome P-450 (CYP) was measured simultaneously by tissue spectrophotometry. Inhaled CO decreased HPV and vasoconstriction induced by the thromboxane mimetic U-46619 but did not alter KCl-induced vasoconstriction. In contrast, concentrations of CORM-2 and CORM-3 used to elicit beneficial effects on the systemic circulation did not affect pulmonary vascular tone. High concentration of CO-RMs or long-term application induced a continuous increase in normoxic pressure. Inhaled CO showed spectral alterations correlating with the inhibition of CYP. In contrast, during application of CORM-2 spectrophotometric signs of interaction with CYP could not be detected. Application of CO-RMs in therapeutic doses in isolated lungs neither decreases pulmonary vascular tone and HPV nor does it induce spectral alterations that are characteristic of CO-inhibited CYP. High doses, however, may cause pulmonary vasoconstriction. [ABSTRACT FROM AUTHOR]
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- 2016
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4. NADPH oxidases-do they play a role in TRPC regulation under hypoxia?
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Malczyk, Monika, Veith, Christine, Schermuly, Ralph, Gudermann, Thomas, Dietrich, Alexander, Sommer, Natascha, Weissmann, Norbert, and Pak, Oleg
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NADPH oxidase ,TRP channels ,HYPOXEMIA ,VASOCONSTRICTION ,VENTILATION ,PULMONARY hypertension - Abstract
In the lung, acute alveolar hypoxia causes hypoxic pulmonary vasoconstriction (HPV) to maintain ventilation perfusion matching and thus optimal oxygenation of blood. In contrast, global chronic hypoxia triggers a pathological thickening of pulmonary arterial walls, called pulmonary vascular remodelling, leading to persistence of pulmonary hypertension (PH). Moreover, ischaemia or hypoxia can lead to a damage of pulmonary endothelial cells with subsequent oedema formation. Alterations in reactive oxygen species (ROS) have been suggested as a crucial mediator of such responses. Among the various sources of cellular ROS production, NADPH oxidases (NOXs) have been found to contribute to these physiological and pathophysiological signalling processes. NOXs are the only known examples that generate ROS as the primary function of the enzyme system. However, the downstream targets of NOX-derived ROS signalling in hypoxia are still not known. Canonical transient receptor potential (TRPC) channels recently have been recognised as directly or indirectly ROS-activated channels and have been shown to be essential for hypoxia-dependent vascular regulatory processes in the lung. Against this background, we here summarise the current knowledge on NOX-mediated TRPC channel signalling during hypoxia in the pulmonary circulation. [ABSTRACT FROM AUTHOR]
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- 2016
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5. Effects of hypercapnia and NO synthase inhibition in sustained hypoxic pulmonary vasoconstriction.
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Ketabchi, Farzaneh, Ghofrani, Hossein A., Schermuly, Ralph T., Seeger, Werner, Grimminger, Friedrich, Egemnazarov, Bakytbek, Shid-Moosavi, S Mostafa, Dehghani, Gholam A., Weissmann, Norbert, and Sommer, Natascha
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HYPERCAPNIA ,NITRIC-oxide synthases ,VASOCONSTRICTION ,PULMONARY gas exchange ,HYPOXEMIA ,ACIDOSIS - Abstract
Background: Acute respiratory disorders may lead to sustained alveolar hypoxia with hypercapnia resulting in impaired pulmonary gas exchange. Hypoxic pulmonary vasoconstriction (HPV) optimizes gas exchange during local acute (0-30 min), as well as sustained (> 30 min) hypoxia by matching blood perfusion to alveolar ventilation. Hypercapnia with acidosis improves pulmonary gas exchange in repetitive conditions of acute hypoxia by potentiating HPV and preventing pulmonary endothelial dysfunction. This study investigated, if the beneficial effects of hypercapnia with acidosis are preserved during sustained hypoxia as it occurs, e.g in permissive hypercapnic ventilation in intensive care units. Furthermore, the effects of NO synthase inhibitors under such conditions were examined. Method: We employed isolated perfused and ventilated rabbit lungs to determine the influence of hypercapnia with or without acidosis (pH corrected with sodium bicarbonate), and inhibitors of endothelial as well as inducible NO synthase on acute or sustained HPV (180 min) and endothelial permeability. Results: In hypercapnic acidosis, HPV was intensified in sustained hypoxia, in contrast to hypercapnia without acidosis when HPV was amplified during both phases. L-NG-Nitroarginine (L-NNA), a non-selective NO synthase inhibitor, enhanced acute as well as sustained HPV under all conditions, however, the amplification of sustained HPV induced by hypercapnia with or without acidosis compared to normocapnia disappeared. In contrast 1400 W, a selective inhibitor of inducible NO synthase (iNOS), decreased HPV in normocapnia and hypercapnia without acidosis at late time points of sustained HPV and selectively reversed the amplification of sustained HPV during hypercapnia without acidosis. Hypoxic hypercapnia without acidosis increased capillary filtration coefficient (Kfc). This increase disappeared after administration of 1400 W. Conclusion: Hypercapnia with and without acidosis increased HPV during conditions of sustained hypoxia. The increase of sustained HPV and endothelial permeability in hypoxic hypercapnia without acidosis was iNOS dependent. [ABSTRACT FROM AUTHOR]
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- 2012
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6. Redox signaling and reactive oxygen species in hypoxic pulmonary vasoconstriction
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Fuchs, Beate, Sommer, Natascha, Dietrich, Alexander, Schermuly, Ralph Theo, Ghofrani, Hossein Ardeschir, Grimminger, Friedrich, Seeger, Werner, Gudermann, Thomas, and Weissmann, Norbert
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OXIDATIVE stress , *PULMONARY blood vessels , *CELLULAR signal transduction , *REACTIVE oxygen species , *HYPOXEMIA , *VASOCONSTRICTION , *MOLECULAR biology , *SMOOTH muscle , *MITOCHONDRIA - Abstract
Abstract: Hypoxic pulmonary vasoconstriction (HPV) is an essential physiological mechanism of the lung that matches blood perfusion with alveolar ventilation to optimize gas exchange. Perturbations of HPV, as may occur in pneumonia or adult respiratory distress syndrome, can cause life-threatening hypoxemia. Despite intensive research for decades, the molecular mechanisms of HPV have not been fully elucidated. Reactive oxygen species (ROS) and changes in the cellular redox state are proposed to link O2 sensing and pulmonary arterial smooth muscle cell contraction underlying HPV. In this regard, mitochondria and NAD(P)H oxidases are discussed as sources of ROS. However, there is controversy whether ROS levels decrease or increase during hypoxia. With this background we summarize the current knowledge on the role of ROS and redox state in HPV. [ABSTRACT FROM AUTHOR]
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- 2010
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7. Effects of hypercapnia with and without acidosis on hypoxic pulmonary vasoconstriction.
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Ketabchi, Farzaneh, Egemnazarov, Bakytbek, Schermuly, Ralph T., Ghofrani, Hossein A., Seeger, Werner, Grimminger, Friedrich, Shid-Moosavi, Mostafa, Dehghani, Gholam A., Weissmann, Norbert, and Sommer, Natascha
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HYPERCAPNIA ,VASOCONSTRICTION ,RESPIRATORY diseases ,LUNG injuries ,HYPOXEMIA ,ACIDOSIS ,LABORATORY rabbits ,PERMEABILITY - Abstract
Acute respiratory disorders and permissive hypercapnic strategy may lead to alveolar hypoxia and hypercapnic acidosis. However, the effects of hypercapnia with or without acidosis on hypoxic pulmonary vasoconstriction (HPV) and oxygen diffusion capacity of the lung are controversial. We investigated the effects of hypercapnic acidosis and hypercapnia with normal pH (pH corrected with sodium bicarbonate) on HPV, capillary permeability, gas exchange, and ventilation-perfusion matching in the isolated ventilated-perfused rabbit lung. No alteration in vascular tone was noted during normoxic hypercapnia with or without acidosis compared with normoxic normocapnia. Hypercapnia with normal pH resulted in a transient increase in HPV during the course of consecutive ventilation maneuvers, whereas hypercapnic acidosis increased HPV over time. Hypercapnic acidosis decreased exhaled NO during hypoxia more than hypercapnia with normal pH and normocapnia, whereas intravascular NO release was unchanged. However, inhibition of NO synthesis by nitro-L-arginine (L-NNA) resulted in a loss of the increased HPV caused by hypercapnic acidosis but not that caused by hypercapnia with normal pH. Furthermore, capillary permeability increased during hypoxic hypercapnia with normal pH but not hypoxic hypercapnic acidosis. This effect was NO-dependent because it disappeared during L-NNA administration. Ventilation-perfusion matching and arterial Po
2 were improved according to the strength of HPV in hypercapnia compared with normocapnia during Tween nebulization-induced lung injury. In conclusion, the increased HPV during hypercapnic acidosis is beneficial to lung gas exchange by improving ventilation-perfusion matching and preserving the capillary barrier function. These effects seem to be linked to NO-mediated pathways. [ABSTRACT FROM AUTHOR]- Published
- 2009
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8. Oxygen sensors in hypoxic pulmonary vasoconstriction
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Weissmann, Norbert, Sommer, Natascha, Schermuly, Ralph Theo, Ghofrani, Hossein Ardeschir, Seeger, Werner, and Grimminger, Friedrich
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HYPOXEMIA , *LIVER diseases , *BLOOD pressure , *LUNG diseases - Abstract
Abstract: Hypoxic pulmonary vasoconstriction (HPV) is an essential mechanism adapting lung perfusion to regional ventilation. Perturbations to HPV, such as those occurring in pneumonia, acute respiratory distress syndrome and liver failure, can result in arterial hypoxemia. Under conditions of general hypoxia, HPV increases pulmonary vascular resistance and thus causes acute pulmonary hypertension. Despite intensive research, the underlying mechanisms of HPV have not been fully elucidated. Deciphering signalling pathways that result in HPV could suggest novel approaches to address a failure of HPV, as well as for the treatment of pulmonary hypertension associated with HPV. Within this context, this review focuses on current concepts in the oxygen sensing mechanisms that underlie HPV. [Copyright &y& Elsevier]
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- 2006
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9. Mitochondrial oxygen sensing of acute hypoxia in specialized cells - Is there a unifying mechanism?
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Pak, Oleg, Nolte, Anika, Knoepp, Fenja, Giordano, Luca, Pecina, Petr, Hüttemann, Maik, Grossman, Lawrence I., Weissmann, Norbert, and Sommer, Natascha
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VASOCONSTRICTION , *CAROTID body , *REACTIVE oxygen species , *CYTOCHROME oxidase , *MITOCHONDRIA , *HYPOXEMIA , *OXYGEN - Abstract
Acclimation to acute hypoxia through cardiorespiratory responses is mediated by specialized cells in the carotid body and pulmonary vasculature to optimize systemic arterial oxygenation and thus oxygen supply to the tissues. Acute oxygen sensing by these cells triggers hyperventilation and hypoxic pulmonary vasoconstriction which limits pulmonary blood flow through areas of low alveolar oxygen content. Oxygen sensing of acute hypoxia by specialized cells thus is a fundamental pre-requisite for aerobic life and maintains systemic oxygen supply. However, the primary oxygen sensing mechanism and the question of a common mechanism in different specialized oxygen sensing cells remains unresolved. Recent studies unraveled basic oxygen sensing mechanisms involving the mitochondrial cytochrome c oxidase subunit 4 isoform 2 that is essential for the hypoxia-induced release of mitochondrial reactive oxygen species and subsequent acute hypoxic responses in both, the carotid body and pulmonary vasculature. This review compares basic mitochondrial oxygen sensing mechanisms in the pulmonary vasculature and the carotid body. • Acute oxygen sensing shares common mechanisms in different cell types. • Mitochondrial reactive oxygen species (ROS) trigger acute hypoxic responses. • Cytochrome c oxidase subunit 4 isoform 2 is essential for acute oxygen sensing. • ROS may originate from complex I or III depending on the cell type. • Further cell-type specific mediators include cellular redox couples. [ABSTRACT FROM AUTHOR]
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- 2022
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10. P-43 - Mitochondria-targeted antioxidant MitoQ intercepts superoxide radical formation under acute hypoxia: Evaluation of the oxidative stress in murine pulmonary arterial smooth muscle cells by electron paramagnetic resonance spectroscopy.
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Scheibe, Susan, Pak, Oleg, Murphy, Michael P., Ghofrani, Hossein A., Seeger, Werner, Grimminger, Friedrich, Schermuly, Ralph T., Weissmann, Norbert, and Sommer, Natascha
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PULMONARY hypertension , *SUPEROXIDE dismutase , *ANTIOXIDANTS , *MITOCHONDRIAL enzymes , *OXIDATIVE stress , *ELECTRON paramagnetic resonance spectroscopy , *HYPOXEMIA , *SMOOTH muscle , *LABORATORY mice - Published
- 2016
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