1. Impact of liver damage on blood-borne variables and pulmonary hemodynamic responses to hypoxia and hyperoxia in anesthetized rats.
- Author
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Sepehrinezhad A, Dehghanian A, Rafati A, and Ketabchi F
- Subjects
- Anesthesia, General, Animals, Biomarkers blood, Blood Pressure, Common Bile Duct surgery, Disease Models, Animal, Estradiol blood, Female, Hepatopulmonary Syndrome blood, Hepatopulmonary Syndrome pathology, Hyperoxia blood, Hypoxia blood, Ligation, Liver pathology, Liver Diseases blood, Liver Diseases pathology, Malondialdehyde blood, Nitric Oxide blood, Portal Vein surgery, Rats, Sprague-Dawley, Respiration, Artificial, Severity of Illness Index, Ventricular Function, Right, Ventricular Pressure, Hemodynamics, Hepatopulmonary Syndrome physiopathology, Hyperoxia physiopathology, Hypoxia physiopathology, Liver Diseases physiopathology, Pulmonary Circulation
- Abstract
Background: Liver disorders may be associated with normal pulmonary hemodynamic, hepatopulmonary syndrome (HPS), or portopulmonary hypertension (POPH). In this study, we aimed to investigate the effect of the severity of liver dysfunctions on blood-borne variables, and pulmonary hemodynamic during repeated ventilation with hyperoxic and hypoxic gases., Methods: Female Sprague Dawley rats were assigned into four groups of Sham (n = 7), portal vein ligation (PPVL, n = 7), common bile duct ligation (CBDL, n = 7), and combination of them (CBDL+ PPVL, n = 7). Twenty-eight days later, right ventricular systolic pressure (RVSP) and systemic blood pressure were recorded in anesthetized animals subjected to repeated maneuvers of hyperoxia (O
2 50%) and hypoxia (O2 10%). Besides, we assessed blood parameters and liver histology., Results: Liver histology score, liver enzymes, WBC and plasma malondialdehyde in the CBDL+PPVL group were higher than those in the CBDL group. Also, the plasma platelet level in the CBDL+PPVL group was lower than those in the other groups. On the other hand, the serum estradiol in the CBDL group was higher than that in the CBDL+PPVL group. All the above parameters in the PPVL group were similar to those in the Sham group. During ventilation with hyperoxia gas, RVSP in the CBDL+PPVL group was higher than the ones in the other groups, and in the CBDL group, it was more than those in the PPVL and Sham groups. Hypoxic pulmonary vasoconstriction (HPV) was not detected in both CBDL+PPVL and CBDL groups, whereas, it retained in the PPVL group., Conclusion: Severe liver damage increases RVSP in the CBDL+PPVL group linked to the high level of ROS, low levels of serum estradiol and platelets or a combination of them. Furthermore, the high RVSP at the noted group could present a reliable animal model for POPH in female rats.- Published
- 2020
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