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Parallel detection of fluorescence confocal microscopy (PDFCM) system based on Digital Micromirror Device (DMD) is reported in this paper in order to realize simultaneous multi-channel imaging and improve detection speed. DMD is added into PDFCM system, working to take replace of the single traditional pinhole in the confocal system, which divides the laser source into multiple excitation beams. The PDFCM imaging system based on DMD is experimentally set up. The multi-channel image of fluorescence signal of potato cells sample is detected by parallel lateral scanning in order to verify the feasibility of introducing the DMD into fluorescence confocal microscope. In addition, for the purpose of characterizing the microscope, the depth response curve is also acquired. The experimental result shows that in contrast to conventional microscopy, the DMD-based PDFCM system has higher axial resolution and faster detection speed, which may bring some potential benefits in the biology and medicine analysis. SCANNING 38:234-239, 2016. © 2015 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR]

Abstract: A new type grating, tilted holographic planar structured dichromated gelatin (DCG) grating with large refractive index modulation, is presented in this paper. The grating is inscribed by superposing two coherent laser beams from the front and the thin side surfaces, respectively. The relationship between the tilted angle and the three types of coupling modes is analyzed when coupling with fiber. Besides, the couple mode theory and experimental investigation are applied on major characteristics analysis of the radiation mode DCG grating of tilted angle 45°. Due to the tilted planar structured grating has large refractive index modulation, it not only offers convenience for connecting (or integrating) with other fiber or waveguide-based devices, but also provide some special characteristics such as low wavelength selectivity (which is required for some applications such as multiple spectrum biomedical imaging). Therefore, this unique grating device has a wide application in optical fields such as for the waveguide-based, parallel, multiple spectrum imaging system. [Copyright &y& Elsevier]

Abstract: Vectorial properties of light have significant effects on the focusing properties, especially for the case of tightly focusing, where the requirements on the ratio of the longitudinal field to the traverse fields (RLT) are different for various applications, such as particle acceleration, micronano-fabrication, fluorescent imaging, determination of three dimensional orientation of individual molecules and so on. Here, utilizing inhomogeneous polarization modulation (IPM) method, we demonstrate how to adjust the numbers of focal spots, the RLT, and the depth of focus (DOF) in terms of requirements and create successfully single or multiple focal spots with dominant transverse or longitudinal fields and small size. In terms of this method, not only the asymmetry of the focal spot for linearly polarized illumination with IPM can be obviously decreased, but also its size is the same as that of the short axis of the elliptical focusing spot for the case without IPM. Moreover, it can not only produce the focal spot with dominant transverse field, low sidelobe, and size slightly smaller than that of the radial polarization, but also can realize the identical resolutions in the transverse direction. In addition, for a focal spot with dominant transverse or longitudinal field, both the subdiffraction sizes (0.4λ or 0.5λ) and the DOF of 6.92λ are also achieved. [Copyright &y& Elsevier]