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• Association of bovine MHC class I (BoLA-A) exons 2–3 and 4–5 allelic variants with immune response to B. abortus S19 is reported in the present study. • The association analysis of various BoLA-A alleles with the immune response revealed that calves with certain defined genotypes induced significantly higher cell-mediated immune responses in terms of lymphocyte proliferation, lymphocyte mediated cytotoxicity and nitrite production. • It is assumed that allelic variants of BoLA-A (exons 2–3 and 4–5) were associated with the differential immune response of calves to B. abortus S19 vaccination. In the present study, we analyzed the immune response of calves to Brucella abortus strain 19 vaccine (S19) and its association with MHC class I (BoLA-A) alleles (exons 2–3 and 4–5). Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used for typing of BoLA-A exon 2–3 with Dde I and Taq I restriction enzymes; and exon 4–5 with Hinf I in 45 crossbred calves. The PCR-RFLP analysis revealed five BoLA-A alleles each for exon 2–3 (A10/A19, A19, A18/19, A18 and A31) and exon 4–5 (A, B, C, D and E). Immune response against B. abortus S19 was assessed at the 4th week post vaccination; antibody response by standard tube agglutination test (STAT) and cell-mediated immunity by lymphocyte proliferation and lymphocyte-mediated cytotoxicity assays. Further, the macrophage function in terms of nitrite production was also analyzed. The association analysis of various BoLA-A alleles with the elicitation of immune response revealed that calves with certain defined genotypes induced significantly higher cell-mediated immune response in terms of lymphocyte proliferation with higher stimulation indices (S.I.) of 1.59 (BoLA-A19), 1.49 (A18/19) and 1.52 (Hinf I-D); lymphocyte mediated cytotoxicity (55.52 % in A19) and nitrite production (43.40 μM in A31). It is assumed that allelic variants of BoLA-A (exons 2–3 and 4–5) were associated with the differential immune response of calves to B. abortus S19 vaccination. Therefore, further studies on association analysis of MHC class-I genes in large number of cattle may generate more information and might be useful for adapting the alternative approach of exploring genetic resistance in the cattle herd against bovine brucellosis. [ABSTRACT FROM AUTHOR]

The present study was undertaken to characterize the distinct immune response in indigenous Ghurrah and exotic Landrace pigs by challenging monocyte-derived macrophages (MDMs) with CSF virus under in-vitro conditions and assessing the variations in the transcriptome profile at 48 h post-infection (hpi). RNA-sequencing was carried out in infected and non-infected MDMs of Ghurrah (n = 3) and Landrace (n = 3) piglets prior- as well as post-stimulation. MDMs of Ghurrah showed greater immune regulation in response to CSF infection with 518 significantly differentially expressed genes (DEG) in infected versus non-infected MDMs, as compared to only 31 DEGs in Landrace MDMs. In Landrace, the principal regulators of inflammation (IL1α , IL1β and TNF) were upregulated in infected cells while in Ghurrah, these were downregulated. Overall, macrophages from indigenous Ghurrah showed more immunological dysregulation in response to virulent CSF virus infection as compared to the exotic Landrace pigs. • The present study aimed to characterize the distinct immune response in indigenous Ghurrah and exotic Landrace pigs against CSF vaccination. • Monocyte-derived macrophages (MDMs) were stimulated with CSF virus under in-vitro conditions. • The variations in the transcriptome profile via RNA-Seq approach were assessed in stimulated cells at 48 h post-infection. • MDMs of Ghurrah showed greater immune regulation in response to CSF infection with 518 significantly differentially expressed genes in infected versus non-infected MDMs, as compared to only 31 DEGs in Landrace MDMs. • In Landrace, the principal regulators of inflammation (IL1α, IL1β and TNF) were upregulated in infected cells while in Ghurrah, these were downregulated. • Macrophages from indigenous Ghurrah showed more immunological dysregulation in response to virulent CSF virus infection as compared to the exotic Landrace pigs. [ABSTRACT FROM AUTHOR]