1. Mesenchymal stem cells modulate albumin-induced renal tubular inflammation and fibrosis
- Author
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Rui Xi Li, Sydney C.W. Tang, Wai Han Yiu, Haojia Wu, Loretta Y.Y. Chan, Yuelin Zhang, Dickson W. L. Wong, Hung-Fat Tse, Qizhou Lian, Joseph Leung, Miao Lin, and Kar Neng Lai
- Subjects
Pathology ,Immunofluorescence ,lcsh:Medicine ,Kidney Tubules, Proximal ,Fibrosis ,Chronic Kidney Disease ,Medicine and Health Sciences ,Blood and Lymphatic System Procedures ,lcsh:Science ,Chemokine CCL5 ,Immune Response ,Chemokine CCL2 ,Multidisciplinary ,Chemistry ,Hepatocyte Growth Factor ,NF-kappa B ,Hematology ,Nephrology ,Cytokines ,Hepatocyte growth factor ,Tumor necrosis factor alpha ,medicine.symptom ,medicine.drug ,Signal Transduction ,Research Article ,Collagen Type IV ,medicine.medical_specialty ,Epithelial-Mesenchymal Transition ,Primary Cell Culture ,Immunology ,Inflammation ,Bone Marrow Cells ,Surgical and Invasive Medical Procedures ,Research and Analysis Methods ,Paracrine signalling ,Albumins ,medicine ,Humans ,Epithelial–mesenchymal transition ,Interleukin 8 ,Immunoassays ,Interleukin-6 ,Tumor Necrosis Factor-alpha ,Mesenchymal stem cell ,Interleukin-8 ,lcsh:R ,Immunity ,Biology and Life Sciences ,Epithelial Cells ,Mesenchymal Stem Cells ,Molecular Development ,medicine.disease ,Actins ,Coculture Techniques ,Gene Expression Regulation ,Tubulointerstitial Disease ,Immune System ,Cancer research ,Immunologic Techniques ,lcsh:Q ,Cell Adhesion Molecules ,Developmental Biology ,Stem Cell Transplantation - Abstract
Bone marrow-derived mesenchymal stem cells (BM-MSCs) have recently shown promise as a therapeutic tool in various types of chronic kidney disease (CKD) models. However, the mechanism of action is incompletely understood. As renal prognosis in CKD is largely determined by the degree of renal tubular injury that correlates with residual proteinuria, we hypothesized that BM-MSCs may exert modulatory effects on renal tubular inflammation and epithelial-to-mesenchymal transition (EMT) under a protein-overloaded milieu. Using a co-culture model of human proximal tubular epithelial cells (PTECs) and BM-MSCs, we showed that concomitant stimulation of BM-MSCs by albumin excess was a prerequisite for them to attenuate albumin-induced IL-6, IL-8, TNF-alpha, CCL-2, CCL-5 overexpression in PTECs, which was partly mediated via deactivation of tubular NF-kappaB signaling. In addition, albumin induced tubular EMT, as shown by E-cadherin loss and alpha-SMA, FN and collagen IV overexpression, was also prevented by BM-MSC co-culture. Albumin-overloaded BM-MSCs per se retained their tri-lineage differentiation capacity and overexpressed hepatocyte growth factor (HGF) and TNFalpha-stimulating gene (TSG)-6 via P38 and NF-kappaB signaling. Albumin-induced tubular CCL-2, CCL-5 and TNF-alpha overexpression were suppressed by recombinant HGF treatment, while the upregulation of alpha-SMA, FN and collagen IV was attenuated by recombinant TSG-6. Neutralizing HGF and TSG-6 abolished the anti-inflammatory and anti-EMT effects of BM-MSC co-culture in albumin-induced PTECs, respectively. In vivo, albumin-overloaded mice treated with mouse BM-MSCs had markedly reduced BUN, tubular CCL-2 and CCL-5 expression, alpha-SMA and collagen IV accumulation independent of changes in proteinuria. These data suggest anti-inflammatory and anti-fibrotic roles of BM-MSCs on renal tubular cells under a protein overloaded condition, probably mediated via the paracrine action of HGF and TSG-6., published_or_final_version
- Published
- 2014