1. Upregulation of CD72 expression on CD19+CD27+ memory B cells by CD40L in primary immune thrombocytopenia.
- Author
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Lyu, Mingen, Hao, Yating, Li, Yang, Lyu, Cuicui, Liu, Wenjie, Li, Huiyuan, Xue, Feng, Liu, Xiaofan, and Yang, Renchi
- Subjects
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CD antigens , *B cells , *IDIOPATHIC thrombocytopenic purpura , *DISEASE remission , *INTERLEUKINS , *INTERLEUKIN-10 - Abstract
CD72 is a co-receptor of B cells and regulates B cell activation. Although aberrant expression of CD72 has been reported in primary immune thrombocytopenia ( ITP), it is uncertain whether this aberrant expression is restricted to specific B cell subsets. Furthermore, the mechanisms that regulate CD72 expression are unknown. In this study, we found higher frequency of CD19+ B cells, CD19+CD27+ memory B cells and lower frequency of CD19+CD27− naive B cells in active ITP patients compared with controls and patients in remission. CD72 expression on CD19+CD27+ cells was upregulated in active ITP patients and correlated with platelet count and anti-platelet autoantibodies. In vitro, CD40L could specifically induce CD72 upregulation on CD19+CD27+ B cells. In combination with CD40L, interleukin ( IL) 10 and BAFF (also termed TNFSF13B) further enhanced CD72 expression on CD19+CD27+ B cells, whereas IL21 reduced CD72 upregulation. CD72 mRNA expression after CD40L stimulation was increased in ITP patients and controls. Significant increase of CD40L on CD4+ T cells was correlated with CD72 expression on CD19+CD27+ B cells in ITP patients. In conclusion, upregulation of CD72 expression on CD27+memory B cells might take part in the pathogenesis of ITP. Elevated CD40L on CD4+ cells combined with cytokines might contribute to the upregulation of CD72 expression on CD27+memory B cells. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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