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Your search keyword '"Keramati F"' showing total 3 results
Start Over Author "Keramati F" Topic immunity, innate
3 results on '"Keramati F"'

1. Chronic HIV infection induces transcriptional and functional reprogramming of innate immune cells.

Author

van der Heijden WA, Van de Wijer L, Keramati F, Trypsteen W, Rutsaert S, Horst RT, Jaeger M, Koenen HJ, Stunnenberg HG, Joosten I, Verweij PE, van Lunzen J, Dinarello CA, Joosten LA, Vandekerckhove L, Netea MG, van der Ven AJ, and de Mast Q

Subjects
Adult, Anti-HIV Agents therapeutic use, Case-Control Studies, Chronic Disease, Cytokines genetics, Cytokines immunology, Female, Gene Expression, HIV Infections drug therapy, HIV Infections genetics, Humans, Inflammation blood, Inflammation immunology, Inflammation virology, Interleukin-1beta genetics, Lipopolysaccharides pharmacology, Male, Middle Aged, Monocytes immunology, Monocytes virology, Neutrophils drug effects, Neutrophils immunology, beta-Glucans metabolism, HIV Infections immunology, Immunity, Innate genetics, Interleukin-1beta blood
Abstract

Chronic inflammation and immune dysfunction play a key role in the development of non-AIDS-related comorbidities. The aim of our study was to characterize the functional phenotype of immune cells in people living with HIV (PLHIV). We enrolled a cross-sectional cohort study of PLHIV on stable antiretroviral therapy and healthy controls. We assessed ex vivo cytokine production capacity and transcriptomics of monocytes and T cells upon bacterial, fungal, and viral stimulation. PLHIV exhibited an exacerbated proinflammatory profile in monocyte-derived cytokines, but not in lymphocyte-derived cytokines. Particularly, the production of the IL-1β to imiquimod, E. coli LPS, and Mycobacterium tuberculosis was increased, and this production correlated with plasma concentrations of high-sensitivity C-reactive protein and soluble CD14. This increase in monocyte responsiveness remained stable over time in subsequent blood sampling after more than 1 year. Transcriptome analyses confirmed priming of the monocyte IL-1β pathway, consistent with a monocyte-trained immunity phenotype. Increased plasma concentrations of β-glucan, a well-known inducer of trained immunity, were associated with increased innate cytokine responses. Monocytes of PLHIV exhibited a sustained proinflammatory immune phenotype with priming of the IL-1β pathway. Training of the innate immune system in PLHIV likely plays a role in long-term HIV complications and provides a promising therapeutic target for inflammation-related comorbidities.

Published
2021
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2. Outcomes of controlled human malaria infection after BCG vaccination.

Author

Walk J, de Bree LCJ, Graumans W, Stoter R, van Gemert GJ, van de Vegte-Bolmer M, Teelen K, Hermsen CC, Arts RJW, Behet MC, Keramati F, Moorlag SJCFM, Yang ASP, van Crevel R, Aaby P, de Mast Q, van der Ven AJAM, Stabell Benn C, Netea MG, and Sauerwein RW

Subjects
Adolescent, Adult, Animals, Anopheles parasitology, B7-2 Antigen metabolism, BCG Vaccine administration & dosage, C-Reactive Protein metabolism, Cytokines blood, Female, GPI-Linked Proteins metabolism, Granzymes blood, HLA-DR Antigens metabolism, Humans, Interferon-gamma blood, Lymphocyte Activation immunology, Male, Parasitemia prevention & control, Plasmodium falciparum immunology, Receptors, IgG metabolism, Vaccination, Young Adult, BCG Vaccine immunology, Immunity, Innate immunology, Immunologic Memory immunology, Killer Cells, Natural immunology, Malaria, Falciparum immunology, Malaria, Falciparum prevention & control
Abstract

Recent evidence suggests that certain vaccines, including Bacillus-Calmette Guérin (BCG), can induce changes in the innate immune system with non-specific memory characteristics, termed 'trained immunity'. Here we present the results of a randomised, controlled phase 1 clinical trial in 20 healthy male and female volunteers to evaluate the induction of immunity and protective efficacy of the anti-tuberculosis BCG vaccine against a controlled human malaria infection. After malaria challenge infection, BCG vaccinated volunteers present with earlier and more severe clinical adverse events, and have significantly earlier expression of NK cell activation markers and a trend towards earlier phenotypic monocyte activation. Furthermore, parasitemia in BCG vaccinated volunteers is inversely correlated with increased phenotypic NK cell and monocyte activation. The combined data demonstrate that BCG vaccination alters the clinical and immunological response to malaria, and form an impetus to further explore its potential in strategies for clinical malaria vaccine development.

Published
2019
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3. beta-Glucan Reverses the Epigenetic State of LPS-Induced Immunological Tolerance

Author

Mihai G. Netea, Cheng Wang, Filomena Matarese, Hendrik G. Stunnenberg, Joost H.A. Martens, Ivo Gut, Paul Flicek, Laura Clarke, Colin Logie, Jelle Zwaag, Ehsan Habibi, Robab Davar, Eva M. Janssen-Megens, Nilofar Sharifi, Vivien Schoonenberg, Wout Megchelenbrink, Matthijs Kox, Rob J.W. Arts, Tatyana Kuznetsova, Peter Pickkers, Simon J. van Heeringen, Farid Keramati, Boris Novakovic, Simon Heath, Shuang-Yin Wang, Bowon Kim, Novakovic, B., Habibi, E., Wang, S. -Y., Arts, R. J. W., Davar, R., Megchelenbrink, W., Kim, B., Kuznetsova, T., Kox, M., Zwaag, J., Matarese, F., van Heeringen, S. J., Janssen-Megens, E. M., Sharifi, N., Wang, C., Keramati, F., Schoonenberg, V., Flicek, P., Clarke, L., Pickkers, P., Heath, S., Gut, I., Netea, M. G., Martens, J. H. A., Logie, C., and Stunnenberg, H. G.

Subjects
0301 basic medicine, Epigenomics, Lipopolysaccharides, beta-Glucans, Lipopolysaccharide, Transcription, Genetic, Macrophage, medicine.medical_treatment, epigenome, lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4], human monocytes, Monocyte, Monocytes, human monocyte, chemistry.chemical_compound, histone modification, Gene Regulatory Networks, GeneralLiterature_REFERENCE(e.g.,dictionaries,encyclopedias,glossaries), Gene Regulatory Network, biology, histone modifications, BLUEPRINT, lipopolysaccharide, Cell Differentiation, Cell biology, macrophages, Histone Code, Histone, Cytokine, Reprogramming, Human, Epigenomic, Sepsi, β-glucan, beta-Glucan, General Biochemistry, Genetics and Molecular Biology, Article, 03 medical and health sciences, trained innate immunity, endotoxin tolerance, Sepsis, medicine, Immune Tolerance, Humans, Epigenetics, Molecular Biology, Innate immune system, Biochemistry, Genetics and Molecular Biology(all), Epigenome, DNA Methylation, Immunity, Innate, 030104 developmental biology, chemistry, Immunology, biology.protein, Immunologic Memory, transcriptome
Abstract

Contains fulltext : 165879.pdf (Publisher’s version ) (Open Access) Innate immune memory is the phenomenon whereby innate immune cells such as monocytes or macrophages undergo functional reprogramming after exposure to microbial components such as lipopolysaccharide (LPS). We apply an integrated epigenomic approach to characterize the molecular events involved in LPS-induced tolerance in a time-dependent manner. Mechanistically, LPS-treated monocytes fail to accumulate active histone marks at promoter and enhancers of genes in the lipid metabolism and phagocytic pathways. Transcriptional inactivity in response to a second LPS exposure in tolerized macrophages is accompanied by failure to deposit active histone marks at promoters of tolerized genes. In contrast, beta-glucan partially reverses the LPS-induced tolerance in vitro. Importantly, ex vivo beta-glucan treatment of monocytes from volunteers with experimental endotoxemia re-instates their capacity for cytokine production. Tolerance is reversed at the level of distal element histone modification and transcriptional reactivation of otherwise unresponsive genes. VIDEO ABSTRACT.

Published
2016

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