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2. Rapid assessment of the antitetanus immune status of a subject using Dot-ELISA

Author

Gazzara D, Mastroeni P, Leonardi Ms, and Bernard Bizzini

Subjects
Pertussis Vaccine, Immune status, Tetanus, Epidemiology, business.industry, Diphtheria Toxoid, Antibody titer, Enzyme-Linked Immunosorbent Assay, medicine.disease, Virology, Antibodies, Bacterial, Rapid assessment, Drug Combinations, Vaccination Campaigns, Immunity, Immunology, Tetanus Toxoid, Medicine, Dot elisa, Pertussis vaccine, Humans, business, Diphtheria-Tetanus-Pertussis Vaccine, medicine.drug
Abstract

We describe a Dot-ELISA system for the rapid, specific and reliable assessment of a subject's antitetanus immune status. The conditions for Dot-ELISA were selected to give positive reactions only for sera with antitetanus antibody titers equal to or higher than 0.06 I.U./ml, considered to represent protection when using "in vitro" methods. This Dot-ELISA method could advantageously be applied to the monitoring of vaccination campaigns, as well as for assessing the antitetanus immune status of the wounded.

Published
1989

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