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2 results on '"Marina Martín-Dacal"'

1. Differential Expression of Fungal Genes Determines the Lifestyle of Plectosphaerella Strains During Arabidopsis thaliana Colonization

Author

Antonio Muñoz-Barrios, Sara Sopeña-Torres, Brisa Ramos, Gemma López, Irene del Hierro, Sandra Díaz-González, Pablo González-Melendi, Hugo Mélida, Vanessa Fernández-Calleja, Verónica Mixão, Marina Martín-Dacal, Marina Marcet-Houben, Toni Gabaldón, Soledad Sacristán, and Antonio Molina

Subjects
Arabidopsis, CAZyme, epiphytic fungus, genome, immunity, pathogenicity, Microbiology, QR1-502, Botany, QK1-989
Abstract

The fungal genus Plectosphaerella comprises species and strains with different lifestyles on plants, such as P. cucumerina, which has served as model for the characterization of Arabidopsis thaliana basal and nonhost resistance to necrotrophic fungi. We have sequenced, annotated, and compared the genomes and transcriptomes of three Plectosphaerella strains with different lifestyles on A. thaliana, namely, PcBMM, a natural pathogen of wild-type plants (Col-0), Pc2127, a nonpathogenic strain on Col-0 but pathogenic on the immunocompromised cyp79B2 cyp79B3 mutant, and P0831, which was isolated from a natural population of A. thaliana and is shown here to be nonpathogenic and to grow epiphytically on Col-0 and cyp79B2 cyp79B3 plants. The genomes of these Plectosphaerella strains are very similar and do not differ in the number of genes with pathogenesis-related functions, with the exception of secreted carbohydrate-active enzymes (CAZymes), which are up to five times more abundant in the pathogenic strain PcBMM. Analysis of the fungal transcriptomes in inoculated Col-0 and cyp79B2 cyp79B3 plants at initial colonization stages confirm the key role of secreted CAZymes in the necrotrophic interaction, since PcBMM expresses more genes encoding secreted CAZymes than Pc2127 and P0831. We also show that P0831 epiphytic growth on A. thaliana involves the transcription of specific repertoires of fungal genes, which might be necessary for epiphytic growth adaptation. Overall, these results suggest that in-planta expression of specific sets of fungal genes at early stages of colonization determine the diverse lifestyles and pathogenicity of Plectosphaerella strains.

Published
2020
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2. Arabidopsis immune responses triggered by cellulose- and mixed-linked glucan-derived oligosaccharides require a group of leucine-rich repeat malectin receptor kinases

Author

Marina Martín‐Dacal, Patricia Fernández‐Calvo, Pedro Jiménez‐Sandoval, Gemma López, María Garrido‐Arandía, Diego Rebaque, Irene del Hierro, Diego José Berlanga, Miguel Ángel Torres, Varun Kumar, Hugo Mélida, Luis F. Pacios, Julia Santiago, Antonio Molina, Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Comunidad de Madrid, Swiss National Science Foundation, Martín-Dacal, Marina, Fernández-Calvo, Patricia, Jiménez-Sandoval, Pedro, Garrido-Arandía, María, Rebaque, Diego, Del Hierro, Irene, Mélida, Hugo, Pacios, Luis F., Santiago, Julia, and Molina, Antonio

Subjects
Mixed-linked glucans (MLGs), Pattern recognition receptors (PRRs), Arabidopsis thaliana, Arabidopsis/metabolism, Protein Serine-Threonine Kinases/metabolism, Arabidopsis Proteins/genetics, Arabidopsis Proteins/metabolism, Leucine/metabolism, Glucans/metabolism, Cellulose/metabolism, Plant Immunity/genetics, Plants/metabolism, Oligosaccharides/metabolism, cellulose, immunity, leucine-reach repeat/Malectin receptor kinase (LRR-MAL RK), mixed-linked glucans (MLGs), oligosaccharides, pattern recognition receptors (PRRs), Genetics, Immunity, Oligosaccharides, Leucine-reach repeat/Malectin receptor kinase (LRR-MAL RK), Cell Biology, Plant Science, Cellulose
Abstract

18 Päg., The plant immune system perceives a diversity of carbohydrate ligands from plant and microbial cell walls through the extracellular ectodomains (ECDs) of pattern recognition receptors (PRRs), which activate pattern-triggered immunity (PTI). Among these ligands are oligosaccharides derived from mixed-linked β-1,3/β-1,4-glucans (MLGs; e.g. β-1,4-D-(Glc)2 -β-1,3-D-Glc, MLG43) and cellulose (e.g. β-1,4-D-(Glc)3 , CEL3). The mechanisms behind carbohydrate perception in plants are poorly characterized except for fungal chitin oligosaccharides (e.g. β-1,4-d-(GlcNAc)6 , CHI6), which involve several receptor kinase proteins (RKs) with LysM-ECDs. Here, we describe the isolation and characterization of Arabidopsis thaliana mutants impaired in glycan perception (igp) that are defective in PTI activation mediated by MLG43 and CEL3, but not by CHI6. igp1-igp4 are altered in three RKs - AT1G56145 (IGP1), AT1G56130 (IGP2/IGP3) and AT1G56140 (IGP4) - with leucine-rich-repeat (LRR) and malectin (MAL) domains in their ECDs. igp1 harbors point mutation E906K and igp2 and igp3 harbor point mutation G773E in their kinase domains, whereas igp4 is a T-DNA insertional loss-of-function mutant. Notably, isothermal titration calorimetry (ITC) assays with purified ECD-RKs of IGP1 and IGP3 showed that IGP1 binds with high affinity to CEL3 (with dissociation constant KD = 1.19 ± 0.03 μm) and cellopentaose (KD = 1.40 ± 0.01 μM), but not to MLG43, supporting its function as a plant PRR for cellulose-derived oligosaccharides. Our data suggest that these LRR-MAL RKs are components of a recognition mechanism for both cellulose- and MLG-derived oligosaccharide perception and downstream PTI activation in Arabidopsis., This work was supported by grant RTI2018-096975-B-I00 from the Spanish Ministry of Science, Innovation and Universities to AM and grant PID-2021-126006OB-100 from the Spanish Ministry of Science and Innovation to AM. This work has also been financially supported by the ‘Severo Ochoa (SO) Programme for Centres of Excellence in R&D’ from the Agencia Estatal de Investigación (AEI) of Spain (grants SEV-2016-0672 (2017-2021) and CEX2020-000999-S (2022-2025) to the CBGP). In the frame of the SO program, HM and PF-C were supported with postdoctoral fellowships. MM-D, DJB and DR were recipients of PhD Fellows PRE2019-088120 and PRE2019-091276 (SEV-2016-0672) from AEI, and IND2017/BIO-7800 from Madrid Regional Government, respectively. Research in the lab of JS was financially supported by the University of Lausanne, the European Research Council (ERC) (grant agreement no. 716358) and the Swiss National Science Foundation (grant no. 310030_204526)., With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2020‐000999‐S)

Published
2023

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