1. Improvement of the Immunochromatographic NG-Test Carba 5 Assay for the Detection of IMP Variants Previously Undetected.
- Author
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Volland H, Girlich D, Laguide M, Gonzalez C, Paris V, Laroche M, Oueslati S, Dortet L, Simon S, and Naas T
- Subjects
- Acinetobacter pathogenicity, Bacterial Proteins genetics, Microbial Sensitivity Tests, Pseudomonas aeruginosa drug effects, Pseudomonas aeruginosa metabolism, beta-Lactamases genetics, Bacterial Proteins metabolism, Immunoassay methods, beta-Lactamases metabolism
- Abstract
Here, we evaluated the immunochromatographic assay NG-Test Carba 5v2 (NG-Biotech), with improved IMP variant detection on 31 IMP producers, representing the different branches of the IMP phylogeny, including 32 OXA-48, 19 KPC, 12 VIM, 14 NDM, and 13 multiple carbapenemase producers (CPs), 13 CPs that were not targeted, and 13 carbapenemase-negative isolates. All tested IMP variants were accurately detected without impairing detection of the other carbapenemases. Thus, NG-Test Carba 5v2 is now well adapted to countries with high IMP prevalence and to the epidemiology of CP- Pseudomonas aeruginosa , where IMPs are most frequently detected., (Copyright © 2019 American Society for Microbiology.)
- Published
- 2019
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