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Your search keyword '"Grose, Randall H."' showing total 2 results
Start Over Author "Grose, Randall H." Topic immunoglobulin e
2 results on '"Grose, Randall H."'

1. Maternal Milk, but Not Formula, Regulates the Immune Response to β-Lactoglobulin in Allergy-Prone Rat Pups.

Author

Tooley, Katie L., El-Merhibi, Adaweyah, Cummins, Adrian G., Grose, Randall H., Lymn, Kerry A., Mark DeNichilo, and Penttila, Irmeli A.

Subjects
BREAST milk, LACTATION, FOOD allergy, CYTOKINES, MESSENGER RNA, MAST cells, IMMUNOGLOBULIN E, DIET, LABORATORY rats
Abstract

Controversy exists regarding the timing of the introduction of allergic foods into the diet. We investigated the immune response of rat pups exposed to β-Iactoglobulin (BLG), one of the main allergenic proteins in cow milk. Brown Norway allergy-prone rats were allocated into groups: dam-reared and unchallenged (DR), DR challenged with BLG via gavage (11 mg/d), or rats fed via gastric cannula a formula containing BLG (11 mg/d). BLG was given from d 4 of life. Rats were killed at d 10, 14, or 21. Sera were assayed for total gE, BLG-specific lgGl, and rat mucosal mast cell protease II (RMCPI I; indicator of mucosal mast cell degranulation). Ileum was assessed for cytokine mRNA. Mesenteric lymph nodes (MLN) were assessed for forkhead boxP3 (Foxp3) and chemokine (C-C motif) receptor 7 )CCR7I expression by real-time PCR and immunostained for Foxp3+ CD4+ regulatory cells. Formula feeding compared with dam-rearing with or without oral BLG challenge resulted in significantly greater serum IgE, BLG-specific IgGl, RMCPII, and intestinal mast cells but reduced MLN Foxp3+ cells, Foxp3, and CCR7 expression and ileal cytokines, interleukin (lL)-4, IL-b, and interferon-γ(P< 0.05). Importantly, giving BLG in the presence of maternal milk resulted in an immune response profile similar to that of unchallenged DR rats but with greater Foxp3 and CCR7 mRNA expression and CD4+ Foxp3+ cells (P < 0.05). We conclude that introducing an allergenic food with breast milk reduces immunological indicators of an allergic response, whereas introduction during formula feeding generates an allergic response. [ABSTRACT FROM AUTHOR]

Published
2009
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2. Maternal milk, but not formula, regulates the immune response to beta-lactoglobulin in allergy-prone rat pups

Author

Irmeli A. Penttila, Adaweyah El-Merhibi, Mark DeNichilo, Randall H. Grose, Kerry A. Lymn, Katie Tooley, Adrian G. Cummins, Tooley, Katie L, El-Merhibi, Adaweyah, Cummins, Adrian G, Grose, Randall H, Lymn, Kerry A, DeNichilo, Mark, and Penttila, Irmeli A

Subjects
Receptors, CCR7, Chemokine, medicine.medical_specialty, medicine.medical_treatment, Medicine (miscellaneous), Lactoglobulins, formula feeding, Immunoglobulin E, medicine.disease_cause, T-Lymphocytes, Regulatory, immune response, Immune system, Ileum, Rats, Inbred BN, Internal medicine, Hypersensitivity, medicine, Animals, Mast Cells, RNA, Messenger, Nutrition and Dietetics, biology, Nutrition & Dietetics, Degranulation, Mast cell, allergic foods, Infant Formula, Rats, Interleukin 10, Cytokine, Endocrinology, medicine.anatomical_structure, Immunoglobulin G, Allergic response, biology.protein, Cytokines, breast milk, Female, Milk Hypersensitivity
Abstract

Controversy exists regarding the timing of the introduction of allergic foods into the diet. We investigated the immune response of rat pups exposed to p-lactoglobulin (BLG), one of the main allergenic proteins in cow milk, Brown Norway allergy-prone rats were allocated into groups: dam-reared and unchallenged (DR, DR challenged with BLG via gavage (11 mg/d), or rats fed via gastric cannula a formula containing BLG (11 mg/d). BLG was given from d 4 of life. Rats were killed at d 10, 14, or 21. Sera were assayed for total IgE, BLG-specific IgG1, and rat mucosal mast cell protease II (RMCPII; indicator of mucosal mast cell degranulation). Ileum was assessed for cytokine mRNA. Mesenteric lymph nodes (MLN) were assessed for forkhead boxP3 (Foxp3) and chemokine (C-C motif) receptor 7 (CCR7) expression by real-time PCR and immunostained for Foxp3(+) CD4(+) regulatory cells. Formula feeding compared with dam-rearing with or without oral BLG challenge resulted in significantly greater serum IgE, BLG-specific IgG1, RMCPII and intestinal mast cells but reduced MLN Foxp3(+) cells, Foxp3, and CCR7 expression and ileal cytokines, interleukin (IL)-4, IL-10, and interferon-gamma (P < 0.05). Importantly, giving BLG in the presence of maternal milk resulted in an immune response profile similar to that of unchallenged DR rats but with greater Foxp3 and CCR7 mRNA expression and CD4(+)Foxp3(+) cells (P< 0.05). We conclude that introducing an allergenic food with breast milk reduces immunological indicators of an allergic response, whereas introduction during formula feeding generates an allergic response. Refereed/Peer-reviewed

Published
2009

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