1. Immunohistochemical analysis of PDGFR-α for wound age determination.
- Author
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Zhang W, Ishida Y, Nosaka M, Kuninaka Y, Ishigami A, Yasuda H, Kofuna A, Kamata T, Goto U, and Kondo T
- Subjects
- Humans, Male, Female, Middle Aged, Adult, Forensic Pathology, Time Factors, Aged, Aged, 80 and over, Receptor, Platelet-Derived Growth Factor alpha metabolism, Immunohistochemistry, Skin injuries, Skin pathology, Skin metabolism, Skin chemistry
- Abstract
Immunohistochemical analysis of platelet-derived growth factor receptor-α (PDGFR-α) was performed on human skin wounds obtained from forensic autopsy cases. Thirty human skin wounds were collected at different post-infliction intervals as follows: Group I, 4 h to 3 days (n = 16); Group II, 4 to 7 days (n = 7); Group III, 9 to 10 days (n = 3); and Group IV, 14 to 20 days (n = 4). Immunopositive reactions for PDGFR-α were not observed in the uninjured human skin specimens. In a semi-quantitative morphometrical analysis, the number of PDGFR-α-positive cells was observed increased in Group II, with the average number of PDGFR-α-positive cells being the highest in Group II. Additionally, in Group II, all specimens showed PDGFR-α-positive cells, with an average number of > 200 cells in five fields of view, suggesting a wound age of 4 to 7 days. Taken together, the immunohistochemical detection of PDGFR-α in human skin wounds can be a useful tool for wound age determination., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
- Published
- 2024
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