6 results on '"Zhao, Ge"'
Search Results
2. CLIC1 overexpression is associated with poor prognosis in pancreatic ductal adenocarcinomas.
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Nina Jia, Shengli Dong, Ge Zhao, Hong Gao, Xueqing Li, Huanhu Zhang, Jia, Nina, Dong, Shengli, Zhao, Ge, Gao, Hong, Li, Xueqing, and Zhang, Huanhu
- Subjects
GENETIC overexpression ,PANCREATIC duct ,ADENOCARCINOMA ,IMMUNOSTAINING ,METASTASIS ,GENE expression ,IMMUNOHISTOCHEMISTRY ,MEMBRANE proteins ,PANCREATIC tumors ,PROGNOSIS ,TUMOR classification ,DUCTAL carcinoma ,KAPLAN-Meier estimator ,TUMOR grading ,DIAGNOSIS - Abstract
Background: Clinical significance of chloride intracellular channel 1 (CLIC1) in pancreatic ductal adenocarcinomas (PDAC) remains largely unknown. This study was performed to assess the expression of CLIC1 in benign and malignant pancreatic lesions, and to assess its clinicopathological significance.Materials and Methods: Tissue samples from resected PDAC (n = 70) and their matched normal pairs were evaluated for CLIC1 expression by immunohistochemical staining. Their expression was correlated with different clinicopathological parameters.Results: CLIC1 expression was significantly higher (67.1%) in PDAC than in adjacent control tissues (25.7%, P < 0.001). High CLIC1 levels were associated with the histological grade (P < 0.001) and tumor size (P < 0.001); but not with sex, age, tumor-node-metastasis (TNM) stage, tumor location, or lymph node metastasis (P < 0.05). Univariate Kaplan-Meier analysis showed that a positive CLIC1 expression was associated with a decreased overall survival (P < 0.01). Multivariate cox regression analysis showed that CLIC1 expression and lymph node metastasis were independent risk factors for disease-free survival.Conclusion: The expression of CLIC1 might be closely related to the carcinogenesis, clinical biological behaviors, and prognosis of pancreatic ductal adenocarcinomas. [ABSTRACT FROM AUTHOR]- Published
- 2016
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3. Time course study of delayed wound healing in a biofilm-challenged diabetic mouse model.
- Author
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Zhao, Ge, Usui, Marcia L., Underwood, Robert A., Singh, Pradeep K., James, Garth A., Stewart, Philip S., Fleckman, Philip, and Olerud, John E.
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ANALYSIS of variance , *ANIMAL experimentation , *BIOFILMS , *BIOLOGICAL models , *DIABETES , *IMMUNOHISTOCHEMISTRY , *INFLAMMATION , *MICE , *PSEUDOMONAS diseases , *REGRESSION analysis , *RESEARCH funding , *T-test (Statistics) , *WOUND healing , *DESCRIPTIVE statistics ,LEG ulcers - Abstract
Bacterial biofilm has been shown to play a role in delaying wound healing of chronic wounds, a major medical problem that results in significant health care burden. A reproducible animal model could be very valuable for studying the mechanism and management of chronic wounds. Our previous work showed that Pseudomonas aeruginosa ( PAO1) biofilm challenge on wounds in diabetic (db/db) mice significantly delayed wound healing. In this wound time course study, we further characterize the bacterial burden, delayed wound healing, and certain aspects of the host inflammatory response in the PAO1 biofilm-challenged db/db mouse model. PAO1 biofilms were transferred onto 2-day-old wounds created on the dorsal surface of db/db mice. Control wounds without biofilm challenge healed by 4 weeks, consistent with previous studies; none of the biofilm-challenged wounds healed by 4 weeks. Of the biofilm-challenged wounds, 64% healed by 6 weeks, and all of the biofilm-challenged wounds healed by 8 weeks. During the wound-healing process, P. aeruginosa was gradually cleared from the wounds while the presence of Staphylococcus aureus (part of the normal mouse skin flora) increased. Scabs from all unhealed wounds contained 107 P. aeruginosa, which was 100-fold higher than the counts isolated from wound beds (i.e., 99% of the P. aeruginosa was in the scab). Histology and genetic analysis showed proliferative epidermis, deficient vascularization, and increased inflammatory cytokines. Hypoxia inducible factor expression increased threefold in 4-week wounds. In summary, our study shows that biofilm-challenged wounds typically heal in approximately 6 weeks, at least 2 weeks longer than nonbiofilm-challenged normal wounds. These data suggest that this delayed wound healing model enables the in vivo study of bacterial biofilm responses to host defenses and the effects of biofilms on host wound healing pathways. It may also be used to test antibiofilm strategies for treating chronic wounds. [ABSTRACT FROM AUTHOR]
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- 2012
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4. Delayed wound healing in diabetic (db/db) mice with Pseudomonas aeruginosa biofilm challenge: a model for the study of chronic wounds.
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Zhao, Ge, Hochwalt, Phillip C., Usui, Marcia L., Underwood, Robert A., Singh, Pradeep K., James, Garth A., Stewart, Philip S., Fleckman, Philip, and Olerud, John E.
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DIABETES complications , *PSEUDOMONAS diseases , *ANALYSIS of variance , *ANIMAL experimentation , *BIOFILMS , *BIOLOGICAL models , *BLOOD sugar , *CHRONIC diseases , *COMPUTER software , *STATISTICAL correlation , *IMMUNOHISTOCHEMISTRY , *MATHEMATICAL models , *MICE , *REGRESSION analysis , *RESEARCH funding , *SERUM albumin , *T-test (Statistics) , *WOUND healing , *WOUNDS & injuries , *THEORY , *DATA analysis , *PHYSIOLOGY , *PATHOLOGICAL physiology - Abstract
Chronic wounds are a major clinical problem that lead to considerable morbidity and mortality. We hypothesized that an important factor in the failure of chronic wounds to heal was the presence of microbial biofilm resistant to antibiotics and protected from host defenses. A major difficulty in studying chronic wounds is the absence of suitable animal models. The goal of this study was to create a reproducible chronic wound model in diabetic mice by the application of bacterial biofilm. Six-millimeter punch biopsy wounds were created on the dorsal surface of diabetic (db/db) mice, subsequently challenged with Pseudomonas aeruginosa (PAO1) biofilms 2 days postwounding, and covered with semiocclusive dressings for 2 weeks. Most of the control wounds were epithelialized by 28 days postwounding. In contrast, none of biofilm-challenged wounds were closed. Histological analysis showed extensive inflammatory cell infiltration, tissue necrosis, and epidermal hyperplasia adjacent to challenged wounds-all indicators of an inflammatory nonhealing wound. Quantitative cultures and transmission electron microscopy demonstrated that the majority of bacteria were in the scab above the wound bed rather than in the wound tissue. The model was reproducible, allowed localized cutaneous wound infections without high mortality, and demonstrated delayed wound healing following a biofilm challenge. This model may provide an approach to study the role of microbial biofilms in chronic wounds as well as the effect of specific biofilm therapy on wound healing. [ABSTRACT FROM AUTHOR]
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- 2010
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5. Potential of chemically modified hydrophilic surface characteristics to support tissue integration of titanium dental implants.
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Schwarz, Frank, Wieland, Marco, Schwartz, Zvi, Zhao, Ge, Rupp, Frank, Geis‐Gerstorfer, Jürgen, Schedle, Andreas, Broggini, Nina, Bornstein, Michael M., Buser, Daniel, Ferguson, Stephen J., Becker, Jürgen, Boyan, Barbara D., and Cochran, David L.
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HYDROPHILIC surfaces ,DENTAL implants ,GROWTH differentiation factors ,TITANIUM ,SURFACE charges - Abstract
In the past, several modifications of specific surface properties such as topography, structure, chemistry, surface charge, and wettability have been investigated to predictably improve the osseointegration of titanium implants. The aim of the present review was to evaluate, based on the currently available evidence, the impact of hydrophilic surface modifications of titanium for dental implants. A surface treatment was performed to produce hydroxylated/hydrated titanium surfaces with identical microstructure to either acid‐etched, or sand‐blasted, large grit and acid‐etched substrates, but with hydrophilic character. Preliminary in vitro studies have indicated that the specific properties noted for hydrophilic titanium surfaces have a significant influence on cell differentiation and growth factor production. Animal experiments have pointed out that hydrophilic surfaces improve early stages of soft tissue and hard tissue integration of either nonsubmerged or submerged titanium implants. This data was also corroborated by the results from preliminary clinical studies. In conclusion, the present review has pointed to a potential of hydrophilic surface modifications to support tissue integration of titanium dental implants. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009 [ABSTRACT FROM AUTHOR]
- Published
- 2009
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6. Elevated expression of PEG10 in human placentas from preeclamptic pregnancies
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Chen, Haiying, Sun, Manni, Zhao, Ge, Liu, Jing, Gao, Wenyan, Si, Si, and Meng, Tao
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PREECLAMPSIA , *GENE expression , *PLACENTA , *PREGNANCY , *IMMUNOHISTOCHEMISTRY , *WESTERN immunoblotting , *POLYMERASE chain reaction , *QUANTITATIVE research - Abstract
Abstract: The aim of this study was to determine qualitative and quantitative changes in paternally expressed gene 10 (PEG10) expression in preeclamptic placentas. Placental tissues were obtained immediately after delivery from women with normal pregnancies (n =20) and patients with preeclampsia (n =20). Quantitative real-time RT-PCR, Western blot analysis and immunohistochemistry were used to determine PEG10 gene expression and localization in placental tissues. Compared with the normal group, PEG10 was highly expressed at both mRNA and protein levels in preeclampsia (P <0.05). In immunohistochemical staining, PEG10 was present in the syncytiotrophoblast, cytotrophoblast, endothelial cell and stroma of all placentas. Notably, the intensity of PEG10 expression in the syncytiotrophoblast, cytotrophoblast and stem villi was much higher in preeclampsia than in normal. In conclusion, elevated expression of PEG10 is likely to be involved in the pathophysiology of preeclampsia. Further studies are needed to elucidate the precise role of PEG10 in preeclampsia. [Copyright &y& Elsevier]
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- 2012
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