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Table of contents A1 Characterization of the immunoallergic profile towards the proteins of the wheat flour in Cuban population Raúl Lázaro Castro Almarales, Mary Carmen Reyes Zamora, Beatriz Tamargo, Damaris Torralba Averoff, Raysa Cruz, Yunia Oliva Diaz, Mirta Alvarez Castello, Alexander Ciria, Alexis Labrada, Maytee Mateo A2 Are peanuts causing food allergy in Cuba? Maytee Mateo, Damaris Torralba Averoff, Raysa Cruz, Yunia Oliva Diaz, Mirta Alvarez Castello, Alexander Ciria, Mary Carmen Reyes Zamora, Beatriz Tamargo, Alexis Labrada A3 Prick test and immunoallergic profile to soy allergens in Cuban population Omar Herrera, Maytee Mateo, Raysa Cruz, Mirta Alvarez Castello, Alexander Ciria, Raúl Lázaro Castro Almarales, Mary Carmen Reyes Zamora, Alexis Labrada A4 Skin sensitization and immunoallergic profile to hen's egg in Cuban population José Severino Rodríguez Canosa, Raysa Cruz, Maytee Mateo, Mirta Alvarez Castello, Alexander Ciria, Raúl Lázaro Castro Almarales, Mary Carmen Reyes Zamora, Alexis Labrada A5 Sensitization to three domestic mites in patients with adverse food events to shellfish Mirta Alvarez Castello, Raúl Lázaro Castro Almarales, Alexis Labrada, Biocen A6 Diagnostic efficacy by skin prick test with allergenic extracts of legumes in Cuban patients Yamilet Ibizate Novales, Ilonka Estruch Fajardo, Alexis Labrada, Maytee Mateo, Armando Ginard A7 Baked egg goods without wheat flour carry an increased risk of reaction Bruce Lanser, Anna Faino, Erwin Gelfand, Pia Hauk A8 Prevalence, incidence and associated risk factors of adverse reaction to food in Cuban infants - a population-based prospective study Silvia Venero Fernández, Julia Urbina, Mirta Alvarez Castello, Raúl Lázaro Castro Almarales, Ramón Suárez Medina, Hermes Fundora Hernández, John Britton, Andrew William Fogarty A9 Microbiome in ice machines and assessing the plasma nanotechnology in breaking the biofilm and improving air quality Nabarun Ghosh, Clinton Ross Bell, Chandini Revanna, Constantine Saadeh, Jeff Bennert, Danius Bouyi, Mitsy Veloz, Nelofar Sherali A10 Characteristics of patients with food allergy in health public service Magna Coelho A11 Allergic rhinitis and asthma index increased in Texas panhandle and AHPCO and plasma nanotechnology as solutions Nabarun Ghosh, Jeff Bennert, Danius Bouyi, Constantine Saadeh, Clinton Ross Bell, Mitsy Veloz, Chandini Revanna, Nelofar Sherali A12 Antigen-specific T follicular helper cells mediate peanut allergy in mice Joseph J. Dolence, Takao Kobayashi, Koji Iijima, Hirohito Kita, Hirohito Kita, Ashli Moore, James Krempski A13 Production of recombinant Mal d 3, a major apple allergen, in Pichia Pastoris, to investigate the impact of the food matrix and post-translational modifications on Mal d 3 immuno-reactivity Roberta Aina, Riccardo Asero, Sabine Pfeifer, Pawel Dubiela, Merima Bublin, Christian Radauer, Piotr Humeniuk, Karin Hoffmann-Sommergruber A14 Reaction to sports drink: no whey! Whey allergy in absence of clinical cow’s milk allergy Frank Eidelman, Ves Dimov, Charl Khalil A15 Food allergy on Tumblr: focus on teenage audience may increase educational impact Ves Dimov, Frank Eidelman, Charl Khalil A16 Changes in IgE levels following one-year immunizations in two children with food allergy Alice E. W. Hoyt, Peter Heymann, Alexander Schuyler, Scott Commins, Thomas Platts-Mills A17 IgE and IgG4 antibodies to cow's milk components in children with eosinophilic esophagitis: higher specific IgG4 antibodies and IgG4:IgE ratios compared with subjects with IgE-mediated food allergy Alexander Schuyler, Patrice Kruszewski, John Russo, Lisa Workman, Thomas Platts-Mills, Elizabeth Erwin, Anubha Tripathi A18 Frequency of Sensitization to Food Allergens in Patients with Rhinitis and Asthma in the National Medical Center La Raza “Dr. Antonio Fraga Mouret”, Mexico City Gabriela Yvette Castellanos, Elizabeth Mendieta, Martín Becerril-Angeles

FEBRUARY 2015 AB70 Abstracts S A T U R D A Y 225 The Role of AHPCO Technology in Reducing Allergic Rhinitis Cases As Air Purifier, Surface Sterilizer and Ice Maker Sterilizer Nabarun K. Ghosh, PhD, Constantine K. Saadeh, MD, FAAAAI, Jeff Bennert, PhD, CTN, Griselda Estrada, MS; West Texas A&M University, Canyon, TX, Allergy ARTS ACCR, Amarillo, TX, AIR OASIS, Amarillo, TX. RATIONALE: Fifteen year’s aeroallergen data that we collected using a Burkard Spore Trap showed a steady increase in aeroallergen concentration in the Texas Panhandle area. Data showed a strong correlation with the allergy and asthma cases that have doubled since 2007. We developed a novel AHPCO or Advanced Hydrated Photocatalytic Oxidation technology to produce filter less air purifiers, surface sterilizer for cell phones and in meat processing facilities and in ice makers to reduce contamination. METHODS: We analyzed the daily aeroallergen by using the coated Melinex tape from the Burkard Volumetric Spore Trap. Exposed, stained Melinex tape was observed under a BX-40 Olympus microscope. We developed and assessed the AHPCO Technology for potential uses as air purification unit, surface sterilizer and net reduction of bacteria, fungi during food processing. Petri dish culture, meat blocks, fruits were placed in the chamber to assess the capacity of sterilization. Images were captured with FITC, TRITC Filters with a BX40 and SZ-CTV Olympus Microscopes and SEM. RESULTS: A fluctuation and gradual shift in aeroallergen index with the warmer climate and a shift in flowering seasons were noticed that contributed the increased allergy cases. AHPCO Technology produces negative ions that can be successfully applied in devices to reduce indoor aeroallergen and bacterial contamination in food processing and ice makers. CONCLUSIONS: Environmental factors contribute to a high concentration of aeroallergen that led to increased allergy cases among the residents of Texas Panhandle. AHPCO Technology can be successfully used in air purifiers, food processing and in ice makers in reducing contamination.

S A T U R D A Y 238 Aerobiology Of Texas Panhandle and Efficiency Of AHPCO Technology As Air Purifier, Surface Sterilizer In Food Processing Dr. Nabarun K. Ghosh, PhD, Dr. Constantine K. Saadeh, MD, FAAAAI, Dr. Jeff Bennert, PhD, CTN, Ms. Griselda Estrada, BS; West Texas A&MUniversity, Canyon, TX, Allergy ARTS ACCR, Amarillo, TX, AIR OASIS, Amarillo, TX. RATIONALE: Allergy and Asthma cases have doubled in Texas Panhandle area since 2007. We have collected aeroallergen samples and characterized for 14 years. We used a novel AHPCO or Advanced Hydrated Photocatalytic Oxidation technology to produce filter less air purifier, surface sterilizer for cell phones and meat processing facilities. METHODS: We have been analyzing the daily aeroallergen by using the coated Melinex tape from the Burkard Volumetric Spore Trap. Exposed, stained Melinex tape was observed under a BX-40 Olympus microscope. We assessed the AHPCO Technology for potential uses as air purification unit, surface sterilizer and net reduction of bacteria, fungi during food processing. A fiberglass chamber was built to evaluate the performance and safety of the air purifiers. Blood, Human cell culture and plant cells were exposed to the AO chamber and The UV chambers to compare the exposures. Petriplates, meat, vegetables were placed in the chamber to assess the capacity of sterilization. Images were captured with FITC, TRITC Filters with a BX40 and SZ-CTVOlympusMicroscopes and SEM. RESULTS: 14 years’ aeroallergen data of Texas Panhandle revealed a gradual shift in aeroallergen index with the warmer climate and a shift in flowering seasons. CONCLUSIONS: Strongwind current, maximum number of feedlots and dry-land agriculture and a gradual shift in flowering season– all contribute to a high concentration of allergic cases among the residents of Texas Panhandle. AHPCO technology proved to be an efficient way to reduce aeroallergen and prevent contamination during food processing.

Background The quality of the environment within buildings is a topic of major importance for public health. Breathing pure and clean air allows us to think more clearly, sleep more soundly, and stay healthier. Studies show that we receive 56% of our energy from the air we breathe, more than from water and food combined. On average we breathe 37 pounds of air a day. It has been established that the use of negative ions in a purification system is an effective means of eradicating aeroallergens in room air. Methods The wall-mount, Inducts and Mobile Sanifier air purification units of Luna are designed to sanitize air, to kill surface mold, bacteria, and viruses in areas up to 26,000 cubic feet. Samples were collected from the clinic rooms to analyze the VOC concentrations using the Luna air purifiers to detect the efficiency in reducing the VOCs in the indoor air. We analyzed the aroallergens and VOCs prevalent in the indoor air in the clinics at the Coulter Animal Hospital, Amarillo, Texas. The data were correlated to the inhalant allergy index before and after running air purifiers that use PCO technology. Results Samples from the clinic rooms to analyze the VOC concentrations using the Luna air purifiers to detect the efficiency in reducing the VOCs in the indoor air. The SKC Pocket Pump and thermal desorption tubes were used to obtain the samples from the indoor air. Pumps were set on 200 mL/min as air flow to estimate the concentration of Acetic acid, Isobutyric acid, Butyric acid, Isovaleric acid, Valeric acid, Hexanoic acid, Phenol, p-cresol, 4-ethyl, 2-amino, Indole, and Skatole. All desorption tube samples were analyzed using a Markes UNITY and Markes Ultra automated thermal desorber (ATD) and a Varian 3800/Saturn 2000 GC with a MS. Slides with double sticky tape were exposed to room air stained with 2% safranin and were observed using a BX-40 Olympus microscope with DP-70 and Image Pro Plus software. Conclusions The data were correlated with the aeroallergen index and the frequency of inhalant allergy cases that showed reduction in allergic rhinitis index on using air purifiers.

Rationale This investigation was aimed at analyzing the relationship between the meteorological conditions, diurnal variations and the fungal aeroallergen data of the North West Texas Panhandle and to find the correlation between the aeroallergen index and frequency of allergic rhinitis recorded in the allergy clinic. Methods Daily mold spore count was determined by analyzing the Melinex tape from the Burkard Volumetric Spore Trap. Samples were analyzed in every 24 hours and mold spore concentration per cubic meter was determined. The data were correlated with maximum and minimum temperature, daily precipitation and peak wind speed. The daily clinical data were collected from the Allergy A.R.T.S. and were correlated with the daily fungal aeroallergen index. Results The most frequent fungal aeroallergens present during Sept. 2002 to Sept. 2003 were fungal spores like Alternaria , ascospores from Pezizales, Dreschlera , Cladosporium , and Curvularia . Mold spore count had an inverse relationship with the daily temperature. Alternaria conidia were the most abundant component of the dry air spora. We found an association between Alternaria concentration and the cases of severe asthma. Fungal spore concentrations did show more susceptibility to meteorological conditions on a daily basis than did pollen concentrations. Ascomycetes concentrations significantly increased in the hours just following a rain shower. Conclusions We found a very significant correlation between the mold spore concentration and the humidity. A little rain increased the spore count significantly. There was a positive correlation between the number of patients' visits with the increase spore index.