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Start Over Author "Boccuni P" Topic immunophenotyping
4 results on '"Boccuni P"'

1. Coexistence of two distinct cell populations (CD56(+)TcRgammadelta(+) and CD56(+)TcRgammadelta(-)) in a case of aggressive CD56(+) lymphoma/leukemia.

Author

Camera A, Pezzullo L, Villa MR, Luciano L, Pane F, Izzo B, Boccuni P, Di Noto R, Del Vecchio L, Salvatore F, and Rotoli B

Subjects
Adult, CD3 Complex blood, Clone Cells, Cytogenetic Analysis, Disease Progression, Humans, Killer Cells, Natural cytology, Killer Cells, Natural immunology, Leukemia blood, Liver Neoplasms blood, Liver Neoplasms immunology, Lymphoma blood, Male, Receptors, Antigen, T-Cell, gamma-delta blood, Receptors, Antigen, T-Cell, gamma-delta genetics, Splenic Neoplasms blood, Splenic Neoplasms immunology, T-Lymphocytes cytology, T-Lymphocytes immunology, CD56 Antigen blood, Immunophenotyping, Leukemia immunology, Lymphoma immunology
Abstract

Background and Objective: Large granular lymphocytes derive from two major lineages: one expressing the CD3 surface antigen (T-lymphocytes), and the other lacking this marker (NK-cells). Although developmental overlaps between natural killer cells and T-cells have been described, malignancies derived from these two cell types are considered as distinct lymphoid disorders., Design and Methods: We report the case of a 30-year old man affected by a lymphoma/leukemia syndrome presenting with hepatosplenic lymphoma which rapidly transformed into aggressive NK-leukemia. Extensive flow cytometry studies and molecular analysis were repeated during the course of the disease, and showed an unexpected changing pattern., Results: At diagnosis, flow cytometry analysis showed the co-existence of two cell populations, one CD56(+), CD3(+), TcRgd(+), and the other CD56(+), CD3(-) and TcRgd(-). Molecular analysis showed that the TcR genes had the same clonally rearranged pattern involving b, g and d genes in both populations. At disease relapse and during the terminal refractory phase, only CD3(-) cells were present., Interpretation and Conclusions: This is an unusual case of CD56(+) aggressive lymphoma/leukemia characterized by the clonal expansion of two phenotypically different cell populations, variably balanced during the course of the disease. The presence of the same TcR genomic rearrangement suggests the origin from a common progenitor able to differentiate along both T- and NK-pathways.

Published
2000

2. Immunophenotypic analysis enables the correct prediction of t(8;21) in acute myeloid leukaemia.

Author

Ferrara F, Di Noto R, Annunziata M, Copia C, Lo Pardo C, Boccuni P, Sebastio L, and Del Vecchio L

Subjects
Acute Disease, Adolescent, Adult, Aged, Female, Humans, Male, Middle Aged, Antigens, CD immunology, Chromosomes, Human, Pair 21 genetics, Chromosomes, Human, Pair 8 genetics, Immunophenotyping methods, Leukemia, Myeloid genetics, Leukemia, Myeloid immunology, Translocation, Genetic
Abstract

Immunophenotypic findings from 14 patients affected by acute myeloid leukaemia (AML) with t(8;21) were compared to those obtained from 79 AML patients with normal or other aberrant karyotypes. Classic lineage markers, adhesion molecules, surface enzymes, stem-cell-related antigens and HLA-DR were investigated. Following evaluation by the Mann-Whitney test, we found that t(8;21) AMLs showed a significantly higher expression of CD19, CD34, CD56, CD45RA and CD54. Conversely, blasts from patients in the control group significantly expressed higher levels of CD45RO, CD33, CD36, CD11b and CD14. In order to split the data at the best cut-off point to achieve the most homogeneous subset with regard to cytogenetic pattern, i.e. t(8;21) or not, the CART (Classification and Regression Trees) method was applied. In the univariate analysis by CART, statistically significant differences were found when CD19 was dichotomized at 10%, CD34 at 37%, CD45RA at 84%, CD54 at 21%, CD56 at 12%, CD36 at 14%, CD45RO at 25%, CD11b at 18% and CD14 at 12%. Once cut-off points were established by CART, we applied the logistic regression model to establish which combination of two or more antigens was most predictive for t(8;21). The combination CD19-CD34 at the cut-off points indicated above correctly classified 92/93 cases (98.9%). The addition of any other antigen combination to the CD19/CD34 model failed to improve the level of prediction. We conclude that AML with t(8;21) displays an exclusive immunophenotype that is highly predictive of the cytogenetic pattern.

Published
1998
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3. Blood cell flow cytometry in paroxysmal nocturnal hemoglobinuria: a tool for measuring the extent of the PNH clone

Author

Alfinito, F., Del Vecchio, L., Rocco, S., Boccuni, P., Pellegrino Musto, Rotoli, B., Alfinito, Fiorella, DEL VECCHIO, L, Rocco, S, Boccuni, P, Musto, P, and Rotoli, B.

Subjects
Adult, Male, Erythrocytes, Glycosylphosphatidylinositols, Neutrophils, T-Lymphocytes, Hemoglobinuria, Paroxysmal, Lipopolysaccharide Receptors, CD59 Antigens, GPI-Linked Proteins, Monocytes, Immunophenotyping, Hemoglobins, Antigens, CD, Antigens, Neoplasm, Reference Values, Humans, B-Lymphocytes, Membrane Glycoproteins, Antibodies, Monoclonal, CD24 Antigen, Middle Aged, Flow Cytometry, Female, Cell Adhesion Molecules, Granulocytes
Abstract

The membrane expression of nine glycosyl phosphatidyl inositol (GPI)-linked molecules was analyzed by flow cytometry on circulating cells from 18 patients affected by paroxysmal nocturnal hemoglobinuria (PNH). The results allowed us to select CD66b, CD14, CD59, CD24 and CD59 monoclonal antibodies as the most suitable reagents for discriminating between normal and PNH cells in PMN, monocytes, RBC and B or T lymphocytes, respectively. In order to assess whether the analysis of distinct cell populations could provide differential information on the extent of the disease, we compared the proportion of residual normal cells in RBC, monocyte and PMN populations. The mean percentage of unaffected cells was higher in RBC as compared to PMN (50.5 +/- 18.7 vs 17.7 +/- 19.7, P0.0001). The proportion of normal PMN was, in turn, significantly greater than that of normal monocytes (17.7 +/- 19.7 vs 8.7 +/- 11.0; P0.05). The percentage of CD14+ monocytes was directly related to Hb concentration and platelet (Plt) count, and inversely to percent lysis at the Ham's test. The percentage of CD66b+ PMN was directly related to Plt count and Hb level, while the percentage of CD59+ RBC was associated, in an inverse fashion, only to the Ham's test. No significant correlation was found between cell marker expression and PMN count, reticulocytosis, bilirubin and serum LDH. By dividing the patients into two groups, according to high (10 percent) or low (10 percent) percentage of CD14+ monocytes, a statistical analysis showed that the main hematological parameters were significantly different.

Published
1996

4. CD66c antigen expression is myeloid restricted in normal bone marrow but is a common feature of CD10+ early-B-cell malignancies

Author

P Boccuni, Bruno Rotoli, L Del Vecchio, M.R. Villa, Felicetto Ferrara, R Di Noto, C. Lo Pardo, Boccuni, P., DI NOTO, Rosa, Lo Pardo, C., Villa, M. R., Ferrara, F., Rotoli, Bruno, and DEL VECCHIO, Luigi

Subjects
Acute promyelocytic leukemia, Pathology, medicine.medical_specialty, Myeloid, Leukemia, T-Cell, Immunology, Antigens, Differentiation, Myelomonocytic, Bone Marrow Cells, Cell Separation, GPI-Linked Proteins, Biochemistry, Immunophenotyping, Antigen, Antigens, CD, Antigens, Neoplasm, hemic and lymphatic diseases, Genetics, medicine, Leukemia, B-Cell, Immunology and Allergy, Humans, B cell, Acute leukemia, Membrane Glycoproteins, business.industry, General Medicine, medicine.disease, Flow Cytometry, Leukemia, Haematopoiesis, medicine.anatomical_structure, Leukopoiesis, Neprilysin, Bone marrow, business, Cell Adhesion Molecules
Abstract

CD66c is a surface (and intracellular) molecule bound to the membrane by a glycosyl-phosphatidylinositol anchor. While its expression on peripheral granulocytes is well recognized, less is known about its distribution in early steps of normal and neoplastic hematopoiesis. We analyzed by flow cytometry cell surface expression of CD66c on bone marrow cells from 4 healthy subjects and on bone marrow or peripheral blood cells from 127 patients with newly diagnosed hematologic malignancies: 70 de novo acute myeloid leukemias (AML), 6 refractory anemias with excess of blasts in transformation, 3 myeloid and 3 lymphoid blastic phases of chronic mye-logenous leukemia, 33 B-lineage and 6 T-lineage acute lymphoblastic leukemias (B- and T-ALL), and 3 B-cell and 3 T-cell non-Hodgkin's lymphomas in the leukemic phase. We found that in normal bone marrow CD66c expression was myeloid restricted, reaching its highest level on promyelocytes. As for de novo AML, slight expression of CD66c was found on 6/25 (24%) AML-M4 and only occasionally in other subgroups. In 9 out of 10 cases of acute promyelocytic leukemia, CD66c was totally absent, but antigen expression was easily detectable following in vitro exposure to all-trans retinoic add. Among lymphoid malignancies, CD10+ early-B-ALL consistently expressed the molecule (20/23 cases, or 87%) whereas both CD10- early-B ALL and Smlg+ B-ALL completely lacked it. Finally, dual staining with CD66c and CD10 proved to be a suitable tool for distinguishing even low percentages of residual leukemic cells (CD10+/CD66c+) from normal regenerating early-B cells (CD10+/CD66c-) in CD10+ early-B-ALL induced into remission.

Published
1998

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