1. Broad immunophenotyping of the murine brain tumor microenvironment.
- Author
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Tomaszewski WH, Waibl-Polania J, Miggelbrink AM, Chakraborty MA, Fecci PE, Sampson JH, and Gunn MD
- Subjects
- Animals, Brain Neoplasms diagnostic imaging, Mice, Mice, Inbred C57BL, Tumor Microenvironment immunology, Brain Neoplasms immunology, Immunophenotyping
- Abstract
Here we present a 14-color flow cytometry panel for the evaluation of 13 myeloid and lymphoid populations within murine glioblastoma samples. Reagents, processing protocols, and downstream analyses were thoroughly validated and optimized to resolve the following populations: T cells (CD4, CD8, CD3), B cells (B220), NK cells (NK1.1), neutrophils (Ly6G), classical and non-classical monocytes (Ly6c, CD43), macrophages (F4/80, CD11b), microglia (CD45-lo, CD11b), and dendritic cells (DCs) (CD11c, MHC class II). In addition, this panel leaves Alexa Fluor 488/FITC open for the inclusion of fluorescent reporters or congenic marker staining., (Copyright © 2021. Published by Elsevier B.V.)
- Published
- 2021
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