1. Solution-mediated phase transition of protein crystals.
- Author
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Suzuki, Yoshihisa, Ikeuchi, Akiho, Taoka, Koichiro, and Sazaki, Gen
- Subjects
- *
PHASE transitions , *CRYSTALLOIDS (Botany) , *ISOMERASES , *PARAFFIN wax , *AQUEOUS solutions - Abstract
A snapshot of the evidence of solution-mediated phase transition of glucose isomerase (GI) crystals more than 7 months after the start of an experiment was successfully captured. The transition to P 2 1 2 1 2 crystals started about 2 months after the first I222 crystals nucleated. To conduct such long-term experiments, we developed novel observation cells with liquid paraffin layers to prevent aqueous GI solutions from evaporation of water. Changes in the weights of cells were measured, and much less evaporation was successfully achieved by continuously adding liquid paraffin as a liquid sealant. [ABSTRACT FROM AUTHOR]
- Published
- 2025
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