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Your search keyword '"Mocchi, Roberto"' showing total 4 results
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4 results on '"Mocchi, Roberto"'

1. Antimicrobial Efficacy Assessment and Rheological Investigation of Two Different Hand Sanitizers Compared with the Standard Reference WHO Formulation 1.

Author

Sommatis, Sabrina, Capillo, Maria Chiara, Maccario, Cristina, Rauso, Raffaele, D'Este, Edoardo, Herrera, Martha, Castiglioni, Mauro, Mocchi, Roberto, and Zerbinati, Nicola

Subjects
ANTI-infective agents, RHEOLOGY, HAND sanitizers, QUALITY control, DISC diffusion tests (Microbiology)
Abstract

(1) Background: recently, the use of alcohol-based hand sanitizers (ABHSs) has become very frequent, and an evaluation of the stability and effectiveness of their formulations is a critical topic which should be carefully considered. (2) Methods: starting from the characterization of the hand sanitizers object of the study, our interest was focused on their rheological behavior in order to confirm their intrinsic features, but also the stability of each formulation in different conditions of shear and temperature; the second aspect concerns the antimicrobial assessment through a panel of in vitro and in vivo experimental trials. (3) Results: rheological investigation confirmed good stability for the two hand sanitizers in gel formula with respect to the reference in liquid formula; the antimicrobial activity evaluation showed good efficacy of each formulation both in vitro and in vivo. (4) Conclusions: altogether, our overview presents a valid quality control assessment to ensure the stability and efficacy of an alcohol-based hand sanitizer. [ABSTRACT FROM AUTHOR]

Published
2023
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2. A Practical Approach for the In Vitro Safety and Efficacy Assessment of an Anti-Ageing Cosmetic Cream Enriched with Functional Compounds.

Author

Zerbinati, Nicola, Sommatis, Sabrina, Maccario, Cristina, Di Francesco, Serena, Capillo, Maria Chiara, Grimaldi, Giulia, Rauso, Raffaele, Herrera, Martha, Bencini, Pier Luca, and Mocchi, Roberto

Subjects
FACIAL creams (Cosmetics), OINTMENTS, ELASTIN, EXTRACELLULAR matrix, MATRIX metalloproteinases, SKIN aging
Abstract

(1) Background: Cosmeceuticals are topical products applied to human skin to prevent skin ageing and maintain a healthy skin appearance. Their effectiveness is closely linked to the compounds present in a final formulation. In this article, we propose a panel of in vitro tests to support the efficacy assessment of an anti-ageing cream enriched with functional compounds. (2) Methods: biocompatibility and the irritant effect were evaluated on reconstructed human epidermis (RHE) and corneal epithelium (HCE) 3D models. After a preliminary MTT assay, normal human dermal fibroblasts (NHDF) and keratinocytes (HaCaT) were used to evaluate the extracellular matrix (ECM) protein synthesis, and interleukin-6 (IL-6) and metalloproteinase-1 (MMP-1) production. (3) Results: data collected showed good biocompatibility and demonstrated the absence of the irritant effect in both 3D models. Therefore, we demonstrated a statistical increase in collagen and elastin productions in NHDF cells. In HaCaT cells, we highlighted an anti-inflammatory effect through a reduction in IL-6 levels in inflammatory stimulated conditions. Moreover, the reduction of MMP-1 production after UV-B radiation was demonstrated, showing significant photo-protection. (4) Conclusion: a multiple in vitro assays approach is proposed for the valid and practical assessment of the anti-ageing protection, anti-inflammatory and biocompatible claims that can be assigned to a cosmetic product containing functional compounds. [ABSTRACT FROM AUTHOR]

Published
2021
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3. In Vitro Evaluation of the Effect of a Not Cross-Linked Hyaluronic Acid Hydrogel on Human Keratinocytes for Mesotherapy

Author

Raffaele Rauso, Roberto Mocchi, Maria Chiara Capillo, Edoardo D'Este, Cristina Maccario, Serena Di Francesco, Nicola Zerbinati, Sabrina Sommatis, Marina Protasoni, Daniela Dalla Gasperina, Zerbinati, Nicola, Sommatis, Sabrina, Maccario, Cristina, Capillo, Maria Chiara, Di Francesco, Serena, Rauso, Raffaele, Protasoni, Marina, D'Este, Edoardo, Dalla Gasperina, Daniela, and Mocchi, Roberto

Subjects
skin, Polymers and Plastics, Angiogenesis, Bioengineering, Inflammation, Pharmacology, medicine.disease_cause, Article, lcsh:Chemistry, Biomaterials, 030207 dermatology & venereal diseases, 03 medical and health sciences, chemistry.chemical_compound, Hyaluronic acid (HA), 0302 clinical medicine, lcsh:General. Including alchemy, Hyaluronic acid, lcsh:Inorganic chemistry, medicine, biorevitalization, lcsh:Science, 030304 developmental biology, 0303 health sciences, integumentary system, Organic Chemistry, aging, in vitro, Hair follicle, lcsh:QD146-197, Mesotherapy, Vascular endothelial growth factor, Aging, Biorevitalization, In vitro, Skin, medicine.anatomical_structure, lcsh:QD1-999, chemistry, mesotherapy, lcsh:Q, Tumor necrosis factor alpha, medicine.symptom, lcsh:QD1-65, Oxidative stress
Abstract

The multicomponent preparations for mesotherapy are based on the principle that skin and hair aging can be prevented by supplying the fundamental substrates for correct cellular functioning, such as nucleotides, vitamins, amino acids, and biomolecules including hyaluronic acid (HA) that promote skin hydration and several biological activities. The study provides evidence for the application of HYDRO DELUXE BIO (Matex Lab S.p.A), a biocompatible hydrogel containing not cross-linked HA, for the treatment of the scalp’s skin by mesotherapy. Using an in vitro model of immortalized human keratinocytes, we studied markers involved in hair aging prevention and growth, such as inflammatory markers, angiogenesis, and oxidative damage. HYDRO DELUXE BIO showed high biocompatibility and the ability to significantly reduce the expression of the inflammation marker interleukin (IL)-8 in Tumor Necrosis Factor (TNF)-stimulated cells. Then, we evaluated angiogenesis, a pivotal event during hair growth, measuring the Vascular Endothelial Growth Factor (VEGF) expression that resulted to be significantly increased in treated cells, suggesting a pro-angiogenetic capability. A protective activity against the oxidative stress agent was showed, increasing the survival rate in treated cells. Concluding, HYDRO DELUXE BIO is suitable for treatment by mesotherapy of the scalp’s skin as it modulates the expression levels of markers involved in the biorevitalization of the hair follicle.

Published
2020

4. In Vitro Evaluation of the Biosafety of Hyaluronic Acid PEG Cross-Linked with Micromolecules of Calcium Hydroxyapatite in Low Concentration

Author

Edoardo D'Este, Cristina Maccario, Alberto Calligaro, Georgi Tchernev, Sabrina Sommatis, Pablo González-Isaza, Damiano Monticelli, Torello Lotti, Raffaele Rauso, Nicola Zerbinati, Uwe Wollina, Katlein França, Roberto Mocchi, Jacopo Lotti, Zerbinati, Nicola, Lotti, Torello, Monticelli, Damiano, Rauso, Raffaele, González-Isaza, Pablo, D'Este, Edoardo, Calligaro, Alberto, Sommatis, Sabrina, Maccario, Cristina, Mocchi, Roberto, Lotti, Jacopo, Wollina, Uwe, Tchernev, Georgi, and França, Katlein

Subjects
0301 basic medicine, Human keratinocyte, chemistry.chemical_element, lcsh:Medicine, Dermatology, Calcium, hyaluronic acid, biosafety, calcium hydroxyapatite, human keratinocytes, Neauvia Stimulate, 030207 dermatology & venereal diseases, 03 medical and health sciences, Biosafety, chemistry.chemical_compound, 0302 clinical medicine, Basic Science, In vivo, PEG ratio, Hyaluronic acid, Medicine, Cytotoxic T cell, Chromatography, business.industry, lcsh:R, General Medicine, In vitro, HaCaT, 030104 developmental biology, chemistry, business
Abstract

OBJECTIVE: Neauvia Stimulate is biocompatible, injectable hyaluronic acid (HA) filler (26 mg/ml) PEG crosslinked with 1% of calcium hydroxyapatite (CaHA) for facial soft-tissue augmentation that provides volume to tissues, followed by process of neocollagenesis for improving skin quality. AIM: The aim of the present study is to evaluate the biosafety of the product (Lot. 160517-26-1/2 PEG) on human keratinocytes cultured in vitro. MATERIAL AND METHODS: The experimental model proposed, despite being an in vitro system, allows the derivation of useful information to predict the possible activity of the product in further in vivo application. Human keratinocytes (HaCaT cells) were treated with the product for 24h at increasing concentrations of product respect to control (untreated cells). RESULTS: The biosafety of the product to be tested has been evaluated performing different methods: MTT test, NRU test, Kenacid Blue assay. Moreover, any possible effect on the structure, morphology, and viability of cells has been evaluated. CONCLUSION: In conclusion, the results obtained by the different methods show that the product Neauvia Stimulate® does not cause any cytotoxic effect and does not affect the correct structure and morphology of cells cultures. OBJECTIVE: Neauvia Stimulate is biocompatible, injectable hyaluronic acid (HA) filler (26 mg/ml) PEG crosslinked with 1% of calcium hydroxyapatite (CaHA) for facial soft-tissue augmentation that provides volume to tissues, followed by process of neocollagenesis for improving skin quality. AIM: The aim of the present study is to evaluate the biosafety of the product (Lot. 160517-26-1/2 PEG) on human keratinocytes cultured in vitro. MATERIAL AND METHODS: The experimental model proposed, despite being an in vitro system, allows the derivation of useful information to predict the possible activity of the product in further in vivo application. Human keratinocytes (HaCaT cells) were treated with the product for 24h at increasing concentrations of product respect to control (untreated cells). RESULTS: The biosafety of the product to be tested has been evaluated performing different methods: MTT test, NRU test, Kenacid Blue assay. Moreover, any possible effect on the structure, morphology, and viability of cells has been evaluated. CONCLUSION: In conclusion, the results obtained by the different methods show that the product Neauvia Stimulate® does not cause any cytotoxic effect and does not affect the correct structure and morphology of cells cultures.

Published
2018

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