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The present investigation observed the sealing ability of low shrinkage composite resins in large and deep cavities, placed and photocured in one increment. Large, deep cavities (5.0 mm diameter and 2.5 mm deep) surrounded by enamel were prepared in bovine teeth, which were then divided into five groups. Groups 1, 2, 3 and 4: acid conditioning + Adper Single Bond (3M/ESPE, St Paul, MN, USA) and restoration with Aelite LS Posterior (BISCO Inc. Schaumburg, IL, USA) (G1); Filtek Z-350 (3M/ESPE,St Paul, MN, USA) (G2); Filtek Z-350 Flow (3M/ESPE, St Paul, MN, USA) (G3); Premisa (KERR Corporation, Orange, CA, USA) (G4). Group 5: Silorane Adhesive system (3M/ESPE, St Paul, MN, USA) + restoration with Filtek Low Shrinkage Posterior P90 (3M/ESPE, St Paul, MN, USA). After polymerization, the teeth were immersed in 0.5% basic fuchsine solution and immediately washed. Using the Imagetool Software, the extent of dye along the margins was calculated as a percentage of total perimeter. The restorations were then transversally sectioned and the depth of dye penetration was calculated in mm, using the same software. Kruskal-Wallis analysis for all groups showed no statistical differences for extent (p = 0.54) or depth (p = 0.8364) of dye penetration. According to this methodology, the socalled low shrinkage composite resins had the same sealing ability compared to regular and flowable nanocomposite materials. [ABSTRACT FROM AUTHOR]

This study evaluated the influence of storage solution on bovine enamel. Forty freshly extracted bovine incisor teeth were used. The teeth were divided into four groups (n=10) following conditions, for 90 days: G1 = distilled water (control group); G2 = artificial saliva; G3 = 0,1% thymol; G4 = saline solution. After storage, the specimens were observed under a scanning electron microscope. The comparative analysis of the specimens revealed a great variation in the structure pattern of the normal enamel. The immersion in artificial saliva caused a larger precipitation of salts in the enamel surface. Increase of superficial porosity, characterized by larger amount of depressions was observed in the group of the saline solution. The specimens submerged in 0,1% thymol, revealed a flat, polished superficial aspect. The authors conclude that the storage solutions altered the dental enamel substrate. [ABSTRACT FROM AUTHOR]