1. A feasible in vitro method to evaluate bacterial infiltration in three implant-abutment connection systems
- Author
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Alexandre Cavalcante de Queiroz, Ivana Barbosa Suffredini, José Dimas de Oliveira, Jefferson de Souza Silva, and Eduardo Fernandes Bondan
- Subjects
biofilm ,bacteria ,infiltration ,streptococcus mutans ,dental implants ,in vitro techniques ,Dentistry ,RK1-715 - Abstract
Introduction: Microorganism infiltration through the im-plant-abutment interface causes oral health problems such as periimplantitis, leading to implant loss. Materials and Methods: A feasible new method to quantify the Streptococcus mutans (S. mutans) infiltration through the implant-abutment interface gap is introduced in the present work. Internal hexagon (IH; n = 10), external hexagon (EH; n = 10), Morse taper (MT; n = 10), and a control for each group (n = 1) were tested. Bacteria suspension was prepared at 1.5x108 CFU/mL (CFU: colony forming units), and the implants were individually submerged up to the connection level, allowing the bacteria to contact it. The abutment was removed, and bacteria count was performed. Results: The implant sets were tested under normal bacterial growth and early and late biofilm growth conditions. Colony-forming units per mL were obtained, and the results were compared among groups. Differences in bacterial count between the MT and EH (p
- Published
- 2024
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