1. Increased Circulating Angiopoietin-Like Protein 8 Levels Are Associated with Thoracic Aortic Dissection and Higher Inflammatory Conditions.
- Author
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Yang Y, Jiao X, Li L, Hu C, Zhang X, Pan L, Yu H, Li J, Chen D, Du J, and Qin Y
- Subjects
- Adult, Aged, Aortic Dissection diagnostic imaging, Angiopoietin-Like Protein 8, Angiopoietin-like Proteins genetics, Animals, Aortic Aneurysm, Thoracic diagnostic imaging, Apoptosis, Biomarkers blood, C-Reactive Protein analysis, Case-Control Studies, Cytokines metabolism, Female, Fibrin Fibrinogen Degradation Products analysis, Humans, Inflammation diagnosis, Macrophages metabolism, Male, Mice, Middle Aged, Muscle, Smooth, Vascular metabolism, Muscle, Smooth, Vascular pathology, Myocytes, Smooth Muscle metabolism, Myocytes, Smooth Muscle pathology, Peptide Hormones genetics, RAW 264.7 Cells, Up-Regulation, Aortic Dissection blood, Angiopoietin-like Proteins blood, Aortic Aneurysm, Thoracic blood, Inflammation blood, Inflammation Mediators blood, Peptide Hormones blood
- Abstract
Purpose: Thoracic aortic dissection (TAD) is characterized by an inflammatory response. Angiopoietin-like protein 8 (ANGPTL8) is a hormone involved in the regulation of lipid metabolism and inflammation. However, the relationship between ANGPTL8 and TAD remains unknown., Methods: This case-control study included 78 TAD patients and 72 controls. The aortic diameter was evaluated by computed tomography and used to assess TAD severity. Circulating ANGPTL8 levels were measured by enzyme-linked immunosorbent assay. Associations of ANGPTL8 with TAD were determined by multivariate logistic regression., Results: Serum ANGPTL8 levels were significantly higher in TAD patients compared with controls (562.50 ± 20.84 vs. 419.70 ± 22.65 pg/mL, respectively; P < 0.001). After adjusting for confounding factors, circulating ANGPTL8 levels were an independent risk factor for TAD (odds ratio = 1.587/100 pg ANGPTL8, 95% confidence interval [CI] = 1.121-2.247, P < 0.001) and positively associated with diameter (β = 1.081/100 pg ANGPTL8, 95% CI = 0.075-2.086, P = 0.035) and high-sensitivity C-reactive protein (hs-CRP) (β = 0.845/100 pg ANGPTL8, 95% CI = 0.020-1.480, P = 0.009). The area under the curve (AUC) on receiver operating characteristic (ROC) analysis of the combination of ANGPTL8, hs-CRP, and D-dimer was 0.927, and the specificity and sensitivity were 98.46% and 79.49%, respectively. ANGPTL8 was significantly increased in TAD tissue compared with controls. In vitro, ANGPTL8 was increased in angiotensin II (AngII)-treated macrophages and vascular smooth muscle cells (VSMCs), while ANGPTL8 siRNA-mediated knockdown decreased inflammatory factors in AngII-treated macrophages and decreased apoptosis in AngII-treated VSMCs., Conclusion: ANGPTL8 is associated with TAD occurrence and development, which may involve pro-inflammatory effects on macrophages. ANGPTL8 combined with D-dimer and hs-CRP might be a useful clinical predictor of TAD., Trial Registration: ChiCTR-COC-17010792 http://www.chictr.org.cn/showproj.aspx?proj=18288.
- Published
- 2020
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