25 results on '"Steele, Chad"'
Search Results
2. CD36, but not G2A, modulates efferocytosis, inflammation, and fibrosis following bleomycin-induced lung injury.
- Author
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Parks BW, Black LL, Zimmerman KA, Metz AE, Steele C, Murphy-Ullrich JE, and Kabarowski JH
- Subjects
- Animals, Apoptosis drug effects, Apoptosis genetics, CD36 Antigens genetics, Cell Cycle Proteins genetics, Fluorescent Antibody Technique, Immunohistochemistry, In Situ Nick-End Labeling, Inflammation genetics, Lung Injury immunology, Lung Injury metabolism, Mice, Mice, Knockout, Receptors, G-Protein-Coupled genetics, Spectrometry, Mass, Electrospray Ionization, Tandem Mass Spectrometry, Bleomycin toxicity, CD36 Antigens metabolism, Cell Cycle Proteins metabolism, Inflammation metabolism, Lung Injury chemically induced, Receptors, G-Protein-Coupled metabolism
- Abstract
Macrophage G2A and CD36 lipid receptors are thought to mediate efferocytosis following tissue injury and thereby prevent excessive inflammation that could compromise tissue repair. To test this, we subjected mice lacking G2A or CD36 receptor to bleomycin-induced lung injury and measured efferocytosis, inflammation, and fibrosis. Loss of CD36 (but not G2A) delayed clearance of apoptotic alveolar cells (mean 78% increase in apoptotic cells 7 days postinjury), potentiated inflammation (mean 56% increase in lung neutrophils and 75% increase in lung KC levels 7 days postinjury, 51% increase in lung macrophages 14 days postinjury), and reduced lung fibrosis (mean 41% and 29% reduction 14 and 21 days postinjury, respectively). Reduced fibrosis in CD36(-/-) mice was associated with lower levels of profibrotic TH2 cytokines (IL-9, IL-13, IL-4), decreased expression of the M2 macrophage marker Arginase-1, and reduced interstitial myofibroblasts. G2A, on the other hand, was required for optimal clearance of apoptotic neutrophils during zymosan-induced peritoneal inflammation (50.3% increase in apoptotic neutrophils and 30.6% increase in total neutrophils 24 h following zymosan administration in G2A(-/-) mice). Thus, CD36 is required for timely removal of apoptotic cells in the context of lung injury and modulates subsequent inflammatory and fibrotic processes relevant to fibrotic lung disease.
- Published
- 2013
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3. Osteopontin modulates inflammation, mucin production, and gene expression signatures after inhalation of asbestos in a murine model of fibrosis.
- Author
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Sabo-Attwood T, Ramos-Nino ME, Eugenia-Ariza M, Macpherson MB, Butnor KJ, Vacek PC, McGee SP, Clark JC, Steele C, and Mossman BT
- Subjects
- Animals, Asbestos, Serpentine adverse effects, Asbestosis genetics, Asbestosis pathology, Bronchioles immunology, Bronchioles metabolism, Bronchioles pathology, Bronchoalveolar Lavage, Disease Models, Animal, Inflammation genetics, Inflammation pathology, Lasers, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Microdissection, Osteopontin genetics, Respiratory Mucosa immunology, Respiratory Mucosa metabolism, Respiratory Mucosa pathology, Up-Regulation, Asbestosis metabolism, Gene Expression Profiling, Inflammation metabolism, Mucins biosynthesis, Osteopontin metabolism
- Abstract
Inflammation and lung remodeling are hallmarks of asbestos-induced fibrosis, but the molecular mechanisms that control these events are unclear. Using laser capture microdissection (LCM) of distal bronchioles in a murine asbestos inhalation model, we show that osteopontin (OPN) is up-regulated by bronchiolar epithelial cells after chrysotile asbestos exposures. In contrast to OPN wild-type mice (OPN(+/+)) inhaling asbestos, OPN null mice (OPN(-/-)) exposed to asbestos showed less eosinophilia in bronchoalveolar lavage fluids, diminished lung inflammation, and decreased mucin production. Bronchoalveolar lavage fluid concentrations of inflammatory cytokines (IL-1β, IL-4, IL-6, IL-12 subunit p40, MIP1α, MIP1β, and eotaxin) also were significantly less in asbestos-exposed OPN(-/-) mice. Microarrays performed on lung tissues from asbestos-exposed OPN(+/+) and OPN(-/-) mice showed that OPN modulated the expression of a number of genes (Col1a2, Timp1, Tnc, Eln, and Col3a1) linked to fibrosis via initiation and cross talk between IL-1β and epidermal growth factor receptor-related signaling pathways. Novel targets of OPN identified include genes involved in cell signaling, immune system/defense, extracellular matrix remodeling, and cell cycle regulation. Although it is unclear whether the present findings are specific to chrysotile asbestos or would be observed after inhalation of other fibers in general, these results highlight new potential mechanisms and therapeutic targets for asbestosis and other diseases (asthma, smoking-related interstitial lung diseases) linked to OPN overexpression., (Copyright © 2011 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.)
- Published
- 2011
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4. Aspergillus terreus accessory conidia are multinucleated, hyperpolarizing structures that display differential dectin staining and can induce heightened inflammatory responses in a pulmonary model of aspergillosis.
- Author
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Deak E, Nelson M, Hernández-Rodríguez Y, Gade L, Baddley J, Momany M, Steele C, and Balajee SA
- Subjects
- Animals, Aspergillus chemistry, Cytokines metabolism, Disease Models, Animal, Histocytochemistry, Inflammation microbiology, Lectins, C-Type, Lung pathology, Macrophages, Alveolar immunology, Macrophages, Alveolar microbiology, Male, Membrane Proteins metabolism, Mice, Mice, Inbred C57BL, Microscopy, Nerve Tissue Proteins metabolism, Rodent Diseases microbiology, Rodent Diseases pathology, Spores, Fungal chemistry, Staining and Labeling, Aspergillus cytology, Cell Nucleus, Inflammation pathology, Pulmonary Aspergillosis pathology, Spores, Fungal cytology, beta-Glucans analysis
- Abstract
In addition to phialidic conidia (PC), A. terreus produces accessory conidia (AC) both in vitro and in vivo. AC are distinct from PC in cell surface architecture, with the AC surfaces displaying more β-glucan, a molecule that can be a trigger for the induction of inflammatory responses. The present study follows β-glucan cell surface presentation throughout the course of germination of both types of conidia, and analyzes the differential capacity of AC and PC to elicit immune responses. Results show that AC display early, increased dectin-1 labeling on their cell surfaces compared to PC, and this differential dectin-1 labeling is sustained on the cell surface from the time of breaking dormancy through early germ tube emergence. Mouse alveolar macrophages showed a stronger inflammatory cytokine/chemokine response when challenged with AC than with PC in both ex vivo and in vivo experiments, correlating with the greater exposure of β-glucan exhibited by AC. Further, histopathologic staining of the lungs from mice challenged with AC demonstrated heightened cell recruitment and increased inflammatory response compared to the lungs of mice challenged with PC. Our study also demonstrates that AC are multinucleate structures with the ability to germinate rapidly, polarizing in multiple directions and producing several hyphal extensions. We present evidence that A. terreus AC are phenotypically distinct from PC and can be potent activators of the innate immune mechanism thus possibly playing a role in this organism's pathogenesis.
- Published
- 2011
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5. Innate immune activation through Nalp3 inflammasome sensing of asbestos and silica.
- Author
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Dostert C, Pétrilli V, Van Bruggen R, Steele C, Mossman BT, and Tschopp J
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- Animals, Humans, Immunity, Interleukin-1beta metabolism, Macrophages immunology, Macrophages metabolism, Mice, NLR Family, Pyrin Domain-Containing 3 Protein, Asbestos immunology, Carrier Proteins physiology, Inflammation immunology, Inflammation Mediators physiology, Silicon Dioxide immunology
- Abstract
The inhalation of airborne pollutants, such as asbestos or silica, is linked to inflammation of the lung, fibrosis, and lung cancer. How the presence of pathogenic dust is recognized and how chronic inflammatory diseases are triggered are poorly understood. Here, we show that asbestos and silica are sensed by the Nalp3 inflammasome, whose subsequent activation leads to interleukin-1beta secretion. Inflammasome activation is triggered by reactive oxygen species, which are generated by a NADPH oxidase upon particle phagocytosis. (NADPH is the reduced form of nicotinamide adenine dinucleotide phosphate.) In a model of asbestos inhalation, Nalp3-/- mice showed diminished recruitment of inflammatory cells to the lungs, paralleled by lower cytokine production. Our findings implicate the Nalp3 inflammasome in particulate matter-related pulmonary diseases and support its role as a major proinflammatory "danger" receptor.
- Published
- 2008
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6. Airway epithelial NF-kappaB activation modulates asbestos-induced inflammation and mucin production in vivo.
- Author
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Haegens A, Barrett TF, Gell J, Shukla A, Macpherson M, Vacek P, Poynter ME, Butnor KJ, Janssen-Heininger YM, Steele C, and Mossman BT
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- Animals, Bronchoalveolar Lavage Fluid chemistry, Bronchoalveolar Lavage Fluid cytology, Cell Differentiation, Cell Proliferation, Cytokines analysis, Cytokines biosynthesis, Disease Models, Animal, Epithelial Cells pathology, I-kappa B Proteins genetics, Mice, Mice, Transgenic, Mutation, NF-KappaB Inhibitor alpha, Asbestos adverse effects, Bronchi cytology, Epithelial Cells metabolism, Inflammation chemically induced, Mucins biosynthesis, NF-kappa B metabolism
- Abstract
To investigate the role of bronchiolar epithelial NF-kappaB activity in the development of inflammation and fibrogenesis in a murine model of asbestos inhalation, we used transgenic (Tg) mice expressing an IkappaBalpha mutant (IkappaBalphasr) resistant to phosphorylation-induced degradation and targeted to bronchial epithelium using the CC10 promoter. Sham and chrysotile asbestos-exposed CC10-IkappaBalphasr Tg(+) and Tg(-) mice were examined for altered epithelial cell proliferation and differentiation, cytokine profiles, lung inflammation, and fibrogenesis at 3, 9, and 40 days. KC, IL-6 and IL-1beta were increased (p < or = 0.05) in bronchoalveolar lavage fluid (BALF) from asbestos-exposed mice, but to a lesser extent (p < or = 0.05) in Tg(+) vs Tg(-) mice. Asbestos also caused increases in IL-4, MIP-1beta, and MCP-1 in BALF that were more elevated (p < or = 0.05) in Tg(+) mice at 9 days. Differential cell counts revealed eosinophils in BALF that increased (p < or = 0.05) in Tg(+) mice at 9 days, a time point corresponding with significantly increased numbers of bronchiolar epithelial cells staining positively for mucus production. At all time points, asbestos caused increased numbers of distal bronchiolar epithelial cells and peribronchiolar cells incorporating the proliferation marker, Ki-67. However, bronchiolar epithelial cell and interstitial cell labeling was diminished at 40 days (p < or = 0.05) in Tg(+) vs Tg(-) mice. Our findings demonstrate that airway epithelial NF-kappaB activity plays a role in orchestrating the inflammatory response as well as cell proliferation in response to asbestos.
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- 2007
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7. Hematopoietic 12/15-lipoxygenase activity negatively contributes to fungal-associated allergic asthma.
- Author
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Makullah, Mgayya, Ellis, Diandra A., Jones, MaryJane, and Steele, Chad
- Subjects
ASTHMA ,NON-communicable diseases ,ANTIASTHMATIC agents ,PNEUMONIA ,ARACHIDONIC acid ,NEUTROPHILS ,CHEMOKINE receptors ,BONE marrow - Abstract
Asthma is one of the most common noncommunicable diseases in the world. Approximately 30% of severe cases are associated with fungal sensitization, often associated with allergy to the opportunistic mold Aspergillus fumigatus. Leukotrienes, immunopathogenic mediators derived from the metabolism of arachidonic acid (AA) by 5-lipoxygenase (5-LOX), are often elevated in severe asthma. As such, these mediators are Food and Drug Administration-approved therapeutic targets of the antiasthmatic drugs Zileuton/Zyflo and Singulair/Montelukast. A second enzyme involved in AA metabolism is 12/15-lipoxygenase (12/15-LOX; Alox15). Here, C57BL/6 wild-type (WT) mice subjected to experimental fungal asthma had increased expression of Alox15 mRNA and increased levels of 12-HETE, a product of 12/15-LOX activity, in the lung when compared with naïve and vehicle-treated mice. Mice deficient in 12/15-LOX (Alox15
-/- ) demonstrated better lung function, as measured by airway hyperresponsiveness (AHR), during fungal asthma. Histological assessment revealed reduced inflammation in the lungs of Alox15-/- mice compared with WT mice, which was corroborated by flow cytometric analysis of multiple myeloid (eosinophils and neutrophils) and lymphoid (CD4 + T and γδ T) cell populations. This was further supported by decreased levels of specific chemokines that promote the recruitment of these cells. Likewise, type 1 and 2, but not type 17 cytokines, were significantly lower in the lungs of Alox15-/- mice. Bone marrow chimera studies revealed that the presence of 12/15-LOX in hematopoietic cells contributed to AHR during fungal asthma. Taken together, our data support the hypothesis that hematopoietic-associated 12/15-LOX contributes to type 1 and 2 responses and exacerbation of allergic fungal asthma. [ABSTRACT FROM AUTHOR]- Published
- 2023
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8. Inhaled Asbestos Exacerbates Atherosclerosis in Apolipoprotein E-Deficient Mice via CD4⁺ T Cells doi:10.1289/ehp.11172
- Author
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Fukagawa, Naomi K., Li, Muyao, Sabo-Attwood, Tara, Timblin, Cynthia R., Butnor, Kelly J., Gagne, Jessica, Steele, Chad, Taatjes, Douglas J., Huber, Sally, and Mossman, Brooke T.
- Published
- 2008
9. Effects of alpha 1-antitrypsin on endotoxin-induced lung inflammation in vivo
- Author
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Subramaniyam, Devipriya, Steele, Chad, Köhnlein, Thomas, Welte, Tobias, Grip, Olof, Matalon, Sadis, and Janciauskiene, Sabina
- Published
- 2010
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10. The chemokine CX3CL1/fractalkine regulates immunopathogenesis during fungal-associated allergic airway inflammation.
- Author
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Godwin, Matthew S., Jones, MaryJane, Blackburn, Jonathan P., Yu, Zhihong, Matalon, Sadis, Hastie, Annette T., Meyers, Deborah A., and Steele, Chad
- Abstract
Individuals that present with difficult-to-control asthma and sensitivity to one or more fungal species are categorized as a subset of severe asthma patients belonging to a group herein referred to as severe asthma with fungal sensitization (SAFS). We have previously reported the identification of numerous cytokines and chemokines that were elevated in human asthmatics that were sensitized to fungi vs. nonfungal sensitized asthmatics. Here, we show that the unique chemokine CX3CL1 (fractalkine) is elevated in both bronchoalveolar lavage fluid and sputum from human asthmatics sensitized to fungi, implicating an association with CX3CL1 in fungal asthma severity. In an experimental model of fungal-associated allergic airway inflammation, we demonstrate that the absence of CX3CR1 signaling unexpectedly resulted in a profound impairment in lung function. Histological assessment of lung tissue revealed an unrestricted inflammatory response that was subsequently characterized by enhanced levels of neutrophils, eosinophils, and inflammatory monocytes. Neutrophilic inflammation correlated with elevated IL-17A, proinflammatory cytokines (TNF-α, IL-1α, and IL-1β), neutrophil survival factors (granulocyte colony-stimulating factor), and neutrophil-targeting chemokines (CCL3 and CCL4). Eosinophilia correlated with elevated type 2 responses (IL-5 and IL-13) whereas inflammatory monocyte levels correlated with elevated type 1 responses (IFN-γ and CXCL9) and survival factors (macrophage colony-stimulating factor). Despite enhanced inflammatory responses, the immunoregulatory cytokine IL-10 and the natural inhibitor of IL-1 signaling, IL-1RA, were significantly elevated rather than impaired. Regulatory T-cell levels were unchanged, as were levels of the anti-inflammatory cytokines IL-35 and IL-38. Taken together, the CX3CL1/CX3CR1 axis preserves lung function during fungal-associated allergic airway inflammation through a nonclassical immunoregulatory mechanism. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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11. Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) and receptors in type 1, type 2 and type 17 inflammation in cross-sectional asthma study.
- Author
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Marks, Michelle, Steele, Chad, Moore, Wendy C., Meyers, Deborah A., Rector, Brian, Ampleford, Elizabeth, Bleecker, Eugene R., and Hastie, Annette T.
- Subjects
DEATH receptors ,TRAILS ,CROSS-sectional method ,NECROSIS ,METHACHOLINE chloride ,ASTHMA ,BIOPSY ,BODY fluids ,BRONCHI ,CARRIER proteins ,CELL receptors ,COMPARATIVE studies ,CYTOKINES ,GENETIC polymorphisms ,INFLAMMATION ,LEUCOCYTES ,RESEARCH methodology ,MEDICAL cooperation ,RESEARCH ,RESPIRATORY measurements ,RESPIRATORY mucosa ,SPUTUM ,EVALUATION research ,SEVERITY of illness index ,LEUKOCYTE count ,FORCED expiratory volume - Abstract
Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) reportedly promotes, or conversely, resolves inflammation in asthma. In this study of TRAIL and cell receptors in sputum, bronchoalveolar lavage and biopsy from subjects in the Severe Asthma Research Program at Wake Forest, the high TRAIL group had significant increases in all leucocytes, and was associated with increased type 1, type 2 and type 17 cytokines, but not type 9 interleukin 9. Two variants at loci in the TRAIL gene were associated with higher sputum levels of TRAIL. Increased TRAIL decoy receptor R3/DcR1 was observed on sputum leucocytes compared with death receptor R1/DR4, suggesting reduced apoptosis and prolonged cellular inflammation. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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12. Tristetraprolin Down-Regulation Contributes to Persistent TNF-Alpha Expression Induced by Cigarette Smoke Extract through a Post-Transcriptional Mechanism.
- Author
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Zhao, Xue-Ke, Che, Pulin, Cheng, Ming-Liang, Zhang, Quan, Mu, Mao, Li, Hong, Luo, Yuan, Liang, Yue-Dong, Luo, Xin-Hua, Gao, Chang-Qing, Jackson, Patricia L., Wells, J. Michael, Zhou, Yong, Hu, Meng, Cai, Guoqiang, Thannickal, Victor J., Steele, Chad, Blalock, J. Edwin, Han, Xiaosi, and Chen, Ching-Yi
- Subjects
TRISTETRAPROLIN ,DOWNREGULATION ,TUMOR necrosis factors ,GENETIC transcription ,HEALTH ,SMOKING ,PROTEIN expression - Abstract
Rationale: Tumor necrosis factor-alpha (TNF-α) is a potent pro-inflammatory mediator and its expression is up-regulated in chronic obstructive pulmonary disease (COPD). Tristetraprolin (TTP) is implicated in regulation of TNF-α expression; however, whether TTP is involved in cigarette smoke-induced TNF-α expression has not been determined. Methods: TTP expression was examined by western blot analysis in murine alveolar macrophages and alveolar epithelial cells challenged without or with cigarette smoke extract (CSE). TNF-α mRNA stability, and the decay of TNF-α mRNA, were determined by real-time quantitative RT-PCR. TNF-α protein levels were examined at the same time in these cells. To identify the molecular mechanism involved, a construct expressing the human beta-globin reporter mRNA containing the TNF-α 3’-untranslated region was generated to characterize the TTP targeted site within TNF-α mRNA. Results: CSE induced TTP down-regulation in alveolar macrophages and alveolar epithelial cells. Reduced TTP expression resulted in significantly increased TNF-α mRNA stability. Importantly, increased TNF-α mRNA stability due to impaired TTP function resulted in significantly increased TNF-α levels in these cells. Forced TTP expression abrogated the increased TNF-α mRNA stability and expression induced by CSE. By using the globin reporter construct containing TNF-α mRNA 3’-untranslated region, the data indicate that TTP directly targets the adenine- and uridine-rich region (ARE) of TNF-α mRNA and negatively regulates TNF-α expression at the post-transcriptional level. Conclusion: The data demonstrate that cigarette smoke exposure reduces TTP expression and impairs TTP function, resulting in significantly increased TNF-α mRNA stability and excessive TNF-α expression in alveolar macrophages and epithelial cells. The data suggest that TTP is a novel post-transcriptional regulator and limits excessive TNF-α expression and inflammatory response induced by cigarette smoke. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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13. Nitrite therapy improves survival postexposure to chlorine gas.
- Author
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Honavar, Jaideep, Doran, Stephen, Joo-Yeun Oh, Steele, Chad, Matalon, Sadis, and Patel, Rakesh P.
- Subjects
PHYSIOLOGICAL effects of chlorine ,NITRITES ,SURVIVAL analysis (Biometry) ,NEUTROPHILS ,HOMEOSTASIS ,OXIDATIVE stress ,THERAPEUTICS - Abstract
Exposure to relatively high levels of chlorine (Cl
2 ) gas can occur in mass-casualty scenarios associated with accidental or intentional release. Recent studies have shown a significant postexposure injury phase to the airways, pulmonary, and systemic vasculatures mediated in part by oxidative stress, inflammation, and dysfunction in endogenous nitric oxide homeostasis pathways. However, there is a need for therapeutics that are amenable to rapid and easy administration in the field and that display efficacy toward toxicity after chlorine exposure. In this study, we tested whether nitric oxide repletion using nitrite, by intramuscular injection after Cl2 exposure, could prevent Cl2 gas toxicity. C57bl/6 male mice were exposed to 600 parts per million Cl2 gas for 45 min, and 24-h survival was determined with or without postexposure intramuscular nitrite injection. A single injection of nitrite (10 mg/kg) administered either 30 or 60 min postexposure significantly improved 24-h survival (from ~20% to 50%). Survival was associated with decreased neutrophil accumulation in the airways. Rendering mice neutropenic before Cl2 exposure improved survival and resulted in loss of nitrite-dependent survival protection. Interestingly, female mice were more sensitive to Cl2 -induced toxicity compared with males and were also less responsive to postexposure nitrite therapy. These data provide evidence for efficacy and define therapeutic parameters for a single intramuscular injection of nitrite as a therapeutic after Cl2 gas exposure that is amenable to administration in mass-casualty scenarios. [ABSTRACT FROM AUTHOR]- Published
- 2014
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14. Chlorine gas exposure increases susceptibility to invasive lung fungal infection.
- Author
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Gessner, Melissa A., Doran, Stephen F., Zhihong Yu, Dunaway, Chad W., Matalon, Sadis, and Steele, Chad
- Abstract
Chlorine (Cl
2 ) is a highly irritating and reactive gas with potential occupational and environmental hazards. Acute exposure to Cl2 induces severe epithelial damage, airway hyperreactivity, impaired alveolar fluid clearance, and pulmonary edema in the presence of heightened inflammation and significant neutrophil accumulation in the lungs. Herein, we investigated whether Cl2 exposure affected the lung antimicrobial immune response leading to increased susceptibility to opportunistic infections. Mice exposed to Cl2 and challenged intratracheally 24 h thereafter with the opportunistic mold Aspergillus fumigatus demonstrated an >500-fold increase in A. fumigatus lung burden 72 h postchallenge compared with A. fumigatus mice exposed to room air. Cl2 -exposed A. fumigatus challenged mice also demonstrated significantly higher lung resistance following methacholine challenge and increased levels of plasma proteins (albumin and IgG) in the bronchoalveolar lavage fluid. Despite enhanced recruitment of inflammatory cells to the lungs of Cl2 -exposed A. fumigatus challenged mice, these cells (>60% of which were neutrophils) demonstrated a profound impairment in generating superoxide. Significantly higher A. fumigatus burden in the lungs of Cl2 exposed mice correlated with enhanced production of IL-6, TNF-α, CXCL1, CCl2 , and CCL3. Surprisingly, however, Cl2 -exposed A. fumigatus challenged mice had a specific impairment in the production of IL-17A and IL-22 in the lungs compared with mice exposed to room air and challenged with A. fumigatus. In summary, our results indicate that Cl2 exposure markedly impairs the antimicrobial activity and inflammatory reactivity of myeloid cells in the lung leading to increased susceptibility to opportunistic pathogens. [ABSTRACT FROM AUTHOR]- Published
- 2013
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15. Titanium oxide nanoparticle instillation induces inflammation and inhibits lung development in mice.
- Author
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Ambalavanan, Namasivayam, Stanishevsky, Andrei, Bulger, Arlene, Halloran, Brian, Steele, Chad, Vohra, Yogesh, and Matalon, Sadis
- Abstract
Nanoparticles are used in an increasing number of biomedical, industrial, and food applications, but their safety profiles in developing organisms, including the human fetus and infant, have not been evaluated. Titanium oxide (TiO
2 ) nanoparticles, which are commonly used in cosmetics, sunscreens, paints, and food, have been shown to induce emphysema and lung inflammation in adult mice. We hypothesized that exposure of newborn mice to TiO2 would induce lung inflammation and inhibit lung development. C57BL/6 mice were exposed to TiO2 (anatase; 8-10 nm) nanoparticles by intranasal instillation as a single dose on postnatal day 4 (P4) or as three doses on postnatal days 4, 7, and 10 (each dose = 1 μg/g body wt). Measurements of lung function (compliance and resistance), development (morphometry), inflammation (histology; multiplex analysis of bronchoalveolar lavage fluid for cytokines; PCR array and multiplex analysis of lung homogenates for cytokines) was performed on postnatal day 14. It was observed that a single dose of TiO2 nanoparticles led to inflammatory cell influx, and multiple doses led to increased inflammation and inhibition of lung development without significant effects on lung function. Macrophages were noted to take up the TiO2 nanoparticles, followed by polymorphonuclear infiltrate. Multiple cytokines and matrix metalloproteinase-9 were increased in lung homogenates, and VEGF was reduced. These results suggest that exposure of the developing lung to nanoparticles may lead to ineffective clearance by macrophages and persistent inflammation with resulting effects on lung development and may possibly impact the risk of respiratory disorders in later life. [ABSTRACT FROM AUTHOR]- Published
- 2013
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16. Characterisation of Innate Fungal Recognition in the Lung.
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Faro-Trindade, Inês, Willment, Janet A., Kerrigan, Ann M., Redelinghuys, Pierre, Hadebe, Sabelo, Reid, Delyth M., Srinivasan, Naren, Wainwright, Helen, Lang, Dirk M., Steele, Chad, and Brown, Gordon D.
- Subjects
FUNGI ,LEUCOCYTES ,PATTERN perception ,INFLAMMATION ,PULMONARY function tests ,ZYMOSAN - Abstract
The innate recognition of fungi by leukocytes is mediated by pattern recognition receptors (PRR), such as Dectin-1, and is thought to occur at the cell surface triggering intracellular signalling cascades which lead to the induction of protective host responses. In the lung, this recognition is aided by surfactant which also serves to maintain the balance between inflammation and pulmonary function, although the underlying mechanisms are unknown. Here we have explored pulmonary innate recognition of a variety of fungal particles, including zymosan, Candida albicans and Aspergillus fumigatus, and demonstrate that opsonisation with surfactant components can limit inflammation by reducing host-cell fungal interactions. However, we found that this opsonisation does not contribute directly to innate fungal recognition and that this process is mediated through non-opsonic PRRs, including Dectin-1. Moreover, we found that pulmonary inflammatory responses to resting Aspergillus conidia were initiated by these PRRs in acidified phagolysosomes, following the uptake of fungal particles by leukocytes. Our data therefore provides crucial new insights into the mechanisms by which surfactant can maintain pulmonary function in the face of microbial challenge, and defines the phagolysosome as a novel intracellular compartment involved in the innate sensing of extracellular pathogens in the lung. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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17. Inflammation precedes the development of human malignant mesotheliomas in a SCID mouse xenograft model.
- Author
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Hillegass, Jedd M., Shukla, Arti, Lathrop, Sherrill A., MacPherson, Maximilian B., Beuschel, Stacie L., Butnor, Kelly J., Testa, Joseph R., Pass, Harvey I., Carbone, Michele, Steele, Chad, and Mossman, Brooke T.
- Subjects
MESOTHELIOMA ,INFLAMMATION ,ASBESTOS fibers ,XENOGRAFTS ,INTRAPERITONEAL injections ,TUMORS in animals ,LABORATORY mice ,DIAGNOSIS - Abstract
Asbestos fibers cause chronic inflammation that may be critical to the development of malignant mesothelioma (MM). Two human MM cell lines (Hmeso, PPM Mill) were used in a SCID mouse xenograft model to assess time-dependent patterns of inflammation and tumor formation. After intraperitoneal (IP) injection of MM cells, mice were euthanized at 7, 14, and 30 days, and peritoneal lavage fluid (PLF) was examined for immune cell profiles and human and mouse cytokines. Increases in human MM-derived IL-6, IL-8, bFGF, and VEGF were observed in mice at 7 days postinjection of either MM line, and a striking neutrophilia was observed at all time points. Free-floating tumor spheroids developed in mice at 14 days, and both spheroids and adherent MM tumor masses occurred in all mice at 30 days. Results suggest that inflammation and cytokine production precede and may be critical to the development of MMs. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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18. Inhaled Asbestos Exacerbates Atherosclerosis in Apolipoprotein E--Deficient Mice via CD4+ T Cells.
- Author
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Fukagawa, Naomi K., Li, Muyao, Sabo-Attwood, Tara, Timblin, Cynthia R., Butnor, Kelly J., Gagne, Jessica, Steele, Chad, Taatjes, Douglas J., Huber, Sally, and Mossman, Brooke T.
- Subjects
CARDIOVASCULAR diseases ,EPIDEMIOLOGICAL research ,INFLAMMATION ,ATHEROSCLEROSIS risk factors ,ASBESTOS ,CHRYSOTILE ,GENETICS - Abstract
BACKGROUND: Associations between air pollution and morbidity/mortality from cardiovascular disease are recognized in epidemiologic and clinical studies, but the mechanisms by which inhaled fibers or particles mediate the exacerbation of atherosclerosis are unclear. OBJECTIVE AND METHODS: To determine whether lung inflammation after inhalation of a well-characterized pathogenic particulate, chrysotile asbestos, is directly linked to exacerbation of atherosclerosis and the mechanisms involved, we exposed apolipoprotein E--deficient (ApoE
--/-- ) mice and ApoE--/-- mice crossed with CD4--/-- mice to ambient air, NIEHS (National Institute of Environmental Health Sciences) reference sample of chrysotile asbestos, or fine titanium dioxide (TiO2 ), a nonpathogenic control particle, for 3, 9, or 30 days. RESULTS: ApoE--/-- mice exposed to inhaled asbestos fibers had approximately 3-fold larger atherosclerotic lesions than did TiO2 -exposed ApoE--/-- mice or asbestos-exposed ApoE--/-- /CD4--/-- doubleknockout (DKO) mice. Lung inflammation and the magnitude of lung fibrosis assessed histologically were similar in asbestos-exposed ApoE--/-- and DKO mice. Monocyte chemoattractant protein-1 (MCP-1) levels were increased in bronchoalveolar lavage fluid and plasma, and plasma concentrations correlated with lesion size (p < 0.04) in asbestos-exposed ApoE--/-- mice. At 9 days, activator protein-1 (AP-1) and nuclear factor-κB (NF-κB), transcription factors linked to inflammation and found in the promoter region of the MCP-1 gene, were increased in aortas of asbestos-exposed ApoE--/-- but not DKO mice. CONCLUSION: Our findings show that the degree of lung inflammation and fibrosis does not correlate directly with cardiovascular effects of inhaled asbestos fibers and support a critical role of CD4+ T cells in linking fiber-induced pulmonary signaling to consequent activation of AP-1-- and NF-κB--regulated genes in atherogenesis. [ABSTRACT FROM AUTHOR]- Published
- 2008
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19. Decreased Asbestos-Induced Lung Inflammation and Fibrosis after Radiation and Bone Marrow Transplant.
- Author
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Levis, Jamie, Loi, Roberto, Butnor, Kelly J., Vacek, Pamela, Steele, Chad, Mossman, Brooke T., and Weiss, Daniel J.
- Published
- 2008
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20. Elevated cardiac hemoglobin expression is associated with a pro-oxidative and inflammatory environment in primary mitral regurgitation.
- Author
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Manzoor, Shajer, Kane, Mariame Selma, Grenett, Maximiliano, Oh, Joo-Yeun, Pat, Betty, Lewis, Clifton, Davies, James E., Steele, Chad, Patel, Rakesh P., and Dell'Italia, Louis J.
- Subjects
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MITRAL valve insufficiency , *GENE expression , *INTERSTITIAL cells , *IN situ hybridization , *HEMOGLOBINS , *HAPTOGLOBINS , *GLOBIN genes - Abstract
Primary mitral regurgitation (PMR) is associated with oxidative and inflammatory myocardial damage. We reported greater exosome hemoglobin (Hb) in pericardial fluid (PCF) versus plasma, suggesting a cardiac source of Hb. Test the hypothesis that Hb is produced in the PMR heart and is associated with increased inflammation. Hb gene expression for subunits alpha (HBA) and beta (HBB) was assessed in right atria (RA), left atria (LA) and left ventricular (LV) tissue from donor hearts (n = 10) and PMR patient biopsies at surgery (n = 11). PMR patients (n = 22) had PCF and blood collected for macrophage markers, pro-inflammatory cytokines, and matrix metalloproteinases (MMPs). In-situ hybridization for HBA mRNA and immunohistochemistry for Hb-alpha (Hbα) and Hb-beta (Hbβ) protein was performed on PMR tissue. HBA and HBB genes are significantly increased (>4-fold) in RA, LA, and LV in PMR vs. normal hearts. In PMR tissue, HBA mRNA is expressed in both LV cardiomyocytes and interstitial cells by in-situ hybridization; however, Hbα and Hbβ protein is only expressed in interstitial cells by immunohistochemistry. PCF oxyHb is significantly increased over plasma along with low ratios (<1.0) of haptoglobin:oxyHb and hemopexin:heme supporting a highly oxidative environment. Macrophage chemotactic protein-1, tumor necrosis factor-α, interleukin-6, and MMPs are significantly higher in PCF vs. plasma. There is increased Hb production in the PMR heart coupled with the inflammatory state of the heart, suggests a myocardial vulnerability of further Hb delivery and/or production during cardiac surgery that could adversely affect LV functional recovery. [Display omitted] • Hemoglobin is produced by cardiomyocytes and interstitial cells in the PMR heart. • Cell-free hemoglobin is elevated and haptoglobin levels are depleted in PCF vs. plasma. • PCF has a greater oxidative environment vs. plasma. • A gradient of pro-inflammatory cytokines, chemoattractants, and MMPs are higher in PCF vs. plasma supporting a myocardial source. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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21. Pneumocystis: Immune recognition and evasion
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Pop, Shannon M., Kolls, Jay K., and Steele, Chad
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PNEUMOCYSTIS pneumonia , *ANTIVIRAL agents , *IMMUNE response , *LUNG infections - Abstract
Abstract: Pulmonary infection caused by the opportunistic fungal organism Pneumocystis continues to be a leading AIDS defining illness. The initiation of highly active antiretroviral therapy (HAART) in the HIV-infected population has led to a significant reduction in the incidence of Pneumocystis pneumonia (PCP), although recent trends suggest the incidence has plateaued rather than decreased. Host defense against Pneumocystis involves a delicate, concerted balance between the inflammatory response and immune-mediated clearance. Innate cellular immunity is a cornerstone in this response as it provides the initial recognition event that precipitates an immune response, ultimately leading to clearance of the organism from the host. This review will focus on carbohydrate moieties found in the Pneumocystis cell wall and the immune events that occur following their recognition. [Copyright &y& Elsevier]
- Published
- 2006
- Full Text
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22. Abstract 12319: Pericardial Fluid Chymase Activity Four Hours Post Cardiac Surgery is Related to Intensive Care Unit and Total Hospital Length of Stay.
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Butts, Brittany, Goeddel, Lee, Steele, Chad, Davies, James, Ferrario, Carlos, Gaggar, Amit, Collawn, James, and Dell'Italia, Louis J
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LENGTH of stay in hospitals , *INTENSIVE care units , *CARDIAC surgery , *CARDIAC intensive care , *HOSPITAL utilization - Abstract
Introduction: The robust cardiac inflammatory response associated with operative trauma, reperfusion injury, and other cardiac surgery insults has a significant impact on post-surgical outcomes and hospital utilization. Chymase activity and inflammatory cytokines are elevated in pericardial fluid (PCF) as early as 4 hours post-surgery at levels several-fold higher than in blood. We hypothesized that the post-surgical pericardial environment may elucidate key biomarkers which associate with risk of adverse postoperative outcomes. Objective: To examine associations between key PCF biomarkers of cardiac injury and inflammation 4 hours after cardiac surgery with intensive care unit (ICU) and total hospital length of stay. Methods: PCF was collected from adult patients (N=30) 4 hours post cardiac surgery. Radiolabeled (125I) angiotensin-(1-12) was used as a substrate for the determination of chymase activity. PCF biomarkers were measured using a Luminex bead based multiplex assay. ICU and total length of stay was determined from the medical record. Zero-truncated Poisson regression models were used to analyze association with length of stay. Results: Mean ICU stay was 2.17 ± 3.8 days and mean total length of stay was 6.41 ± 1.3 days. Univariate regression analysis demonstrated that the Society of Thoracic Surgeons Predicted Risk of Morbidity and Mortality Score (STS-PROM), PCF chymase activity, and chemokine ligand 6 (CXCL6) were independent predictors of both ICU and total hospital length of stay. Multivariable models including STS-PROM as a predictor demonstrated that 4-hour PCF chymase activity had the best fit model for both ICU and total length of stay. Conclusions: This exploratory study found that inflammatory markers in PCF 4 hours after cardiac surgery were related to both ICU and total hospital length of stay. Chymase activity had the best fit relationship with length of stay, suggesting 4-hour chymase activity may improve prediction of patient ICU utilization after cardiac surgery. Monitoring post-operative pericardial drainage for inflammatory biomarkers may be a potential tool for predicting outcomes after cardiac surgery and would benefit individual patients for improved management of care and optimization of resource utilization. [ABSTRACT FROM AUTHOR]
- Published
- 2018
23. Increased Inflammation in Pericardial Fluid Persists 48 Hours After Cardiac Surgery.
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Butts, Brittany, Goeddel, Lee A., George, David J., Steele, Chad, Davies, James E., Chih-Chang Wei, Varagic, Jasmina, George, James F., Ferrario, Carlos M., Melby, Spencer J., Dell'Italia, Louis J., and Wei, Chih-Chang
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PERICARDIUM , *ATRIAL fibrillation , *INFLAMMATION , *BLOOD - Abstract
The article focuses on the complications surrounding cardiac surgery, and discusess the ways for preventing the same. Topics discussed include monitoring of blood proinflammatory factors in pericardial fluid after cardiac surgery over time; its adverse effects on atrial fibrillation; and increased neutrophil infiltration after the post cardiac surgery.
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- 2017
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24. Serum (1→3)-β-D-Glucan Levels in HIV-Infected Individuals Are Associated With Immunosuppression, Inflammation, and Cardiopulmonary Function.
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Morris, Alison, Hillenbrand, Maria, Finkelman, Malcolm, George, M. Patricia, Singh, Vikas, Kessinger, Cathy, Lucht, Lorrie, Busch, Michelle, McMahon, Deborah, Weinman, Renee, Steele, Chad, Norris, Karen A., and Gingo, Matthew R.
- Abstract
Translocation of gastrointestinal bacteria in HIV-infected individuals is associated with systemic inflammation, HIV progression, mortality, and comorbidities. HIV-infected individuals are also susceptible to fungal infection and colonization, but whether fungal translocation occurs and influences HIV progression or comorbidities is unknown.Serum (1→3)-β-D-glucan (BG) was measured by a Limulus Amebocyte Lysate assay (Fungitell) in 132 HIV-infected outpatients. Selected plasma cytokines and markers of peripheral T-cell activation were measured. Pulmonary function testing and Doppler echocardiography were performed. Relationship of high (≥40 pg/mL) and low (<40 pg/mL) levels of BG with HIV-associated variables, inflammation markers, and pulmonary function and pulmonary hypertension measures were determined.Forty-eight percent of patients had detectable BG, and 16.7% had high levels. Individuals with high BG were more likely to have CD4 counts less than 200 cells/μL (31.8% vs. 8.4%, P = 0.002), had higher log10 HIV viral levels (2.85 vs. 2.13 log copies/mL, P = 0.004), and were less likely to use antiretroviral therapy (68.2% vs. 90.0%, P = 0.006). Plasma IL-8 (P = 0.033), TNF-α (P = 0.029), and CD8+CD38+ (P = 0.046) and CD8+HLA-DR+ (P = 0.029) were also increased with high levels. Abnormalities in diffusing capacity (P = 0.041) and in pulmonary artery pressures (P = 0.006 for pulmonary artery systolic pressure and 0.013 for tricuspid regurgitant velocity) were more common in those with high BG.We found evidence of peripheral fungal cell wall polysaccharides in an HIV-infected cohort. We also demonstrated an association between high serum BG, HIV-associated immunosuppression, inflammation, and cardiopulmonary comorbidity. These results implicate a new class of pathogen in HIV-associated microbial translocation and suggest a role in HIV progression and comorbidities. [ABSTRACT FROM AUTHOR]
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- 2012
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25. Administration of nitrite after chlorine gas exposure prevents lung injury: Effect of administration modality
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Samal, Andrey A., Honavar, Jaideep, Brandon, Angela, Bradley, Kelley M., Doran, Stephen, Liu, Yanping, Dunaway, Chad, Steele, Chad, Postlethwait, Edward M., Squadrito, Giuseppe L., Fanucchi, Michelle V., Matalon, Sadis, and Patel, Rakesh P.
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NITRITES , *CHLORINE , *LUNG injury prevention , *LABORATORY rats , *INTRAPERITONEAL injections , *HISTOLOGY , *LEUCOCYTES - Abstract
Abstract: Cl2 gas toxicity is complex and occurs during and after exposure, leading to acute lung injury (ALI) and reactive airway syndrome (RAS). Moreover, Cl2 exposure can occur in diverse situations encompassing mass casualty scenarios, highlighting the need for postexposure therapies that are efficacious and amenable to rapid and easy administration. In this study, we assessed the efficacy of a single dose of nitrite (1mg/kg) to decrease ALI when administered to rats via intraperitoneal (ip) or intramuscular (im) injection 30min after Cl2 exposure. Exposure of rats to Cl2 gas (400ppm, 30min) significantly increased ALI and caused RAS 6–24h postexposure as indexed by BAL sampling of lung surface protein and polymorphonucleocytes (PMNs) and increased airway resistance and elastance before and after methacholine challenge. Intraperitoneal nitrite decreased Cl2-dependent increases in BAL protein but not PMNs. In contrast im nitrite decreased BAL PMN levels without decreasing BAL protein in a xanthine oxidoreductase-dependent manner. Histological evaluation of airways 6h postexposure showed significant bronchial epithelium exfoliation and inflammatory injury in Cl2-exposed rats. Both ip and im nitrite improved airway histology compared to Cl2 gas alone, but more coverage of the airway by cuboidal or columnar epithelium was observed with im compared to ip nitrite. Airways were rendered more sensitive to methacholine-induced resistance and elastance after Cl2 gas exposure. Interestingly, im nitrite, but not ip nitrite, significantly decreased airway sensitivity to methacholine challenge. Further evaluation and comparison of im and ip therapy showed a twofold increase in circulating nitrite levels with the former, which was associated with reversal of post-Cl2 exposure-dependent increases in circulating leukocytes. Halving the im nitrite dose resulted in no effect in PMN accumulation but significant reduction of BAL protein levels, indicating a distinct nitrite dose dependence for inhibition of Cl2-dependent lung permeability and inflammation. These data highlight the potential for nitrite as a postexposure therapeutic for Cl2 gas-induced lung injury and also suggest that administration modality is a key consideration in nitrite therapeutics. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
- View/download PDF
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