1. Sulfated Cyclocarya paliurus polysaccharides markedly attenuates inflammation and oxidative damage in lipopolysaccharide-treated macrophage cells and mice.
- Author
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Wang Z, Xie J, Yang Y, Zhang F, Wang S, Wu T, Shen M, and Xie M
- Subjects
- Animals, Antioxidants metabolism, Cell Survival drug effects, Cytokines metabolism, Lipopolysaccharides, Liver drug effects, Liver physiopathology, Macrophages drug effects, Macrophages metabolism, Malondialdehyde metabolism, Mice, Models, Biological, Molecular Weight, Nitric Oxide metabolism, Phagocytosis drug effects, Polysaccharides chemistry, Polysaccharides pharmacology, RAW 264.7 Cells, Spectroscopy, Fourier Transform Infrared, Sulfates chemistry, Sulfates pharmacology, Superoxide Dismutase metabolism, Viscera drug effects, Viscera pathology, Inflammation drug therapy, Inflammation pathology, Juglandaceae chemistry, Macrophages pathology, Oxidative Stress drug effects, Polysaccharides therapeutic use, Sulfates therapeutic use
- Abstract
Natural polysaccharides and their modified derivatives are crucial supplements to the prevention of inflammation. This study aimed to evaluate the effect of sulfated modification on the anti-inflammatory and anti-oxidative activities of Cyclocarya paliurus polysaccharides (CP). A sulfated CP, S-CP
1-4 was obtained using chlorosulfonic acid-pyridine method. The chemical components and FT-IR spectrum confirmed that sulfated group was synthesized to the polysaccharide chains successfully. S-CP1-4 was found to inhibit nitric oxide production, phagocytic activity and the release of interleukin (IL)-6 and IL-1β in lipopolysaccharide-treated macrophage cells, RAW 264.7. S-CP1-4 significantly decreased the secretion of IL-6 and TNF-α and the thymus and spleen indexes, and increased the production of IL-10 in lipopolysaccharide-treated mice. S-CP1-4 could better protect the liver by inhibiting the activities of alanine aminotransferase and aspartate aminotransferase, and malondialdehyde level while increasing the superoxide dismutase activity and total anti-oxidative capacity. These results suggested that S-CP1-4 may be an effective anti-inflammatory agent, and sulfated modification may be a reliable method for the development of food supplements.- Published
- 2017
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