1. Salutary effect of kallistatin in salt-induced renal injury, inflammation, and fibrosis via antioxidative stress.
- Author
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Shen B, Hagiwara M, Yao YY, Chao L, and Chao J
- Subjects
- Animals, Cells, Cultured, Collagen metabolism, Disease Models, Animal, Fibrosis, Inflammation chemically induced, Inflammation pathology, Kidney Diseases chemically induced, Kidney Diseases pathology, Kidney Tubules, Proximal metabolism, Male, Mesangial Cells metabolism, Nitric Oxide Synthase Type III metabolism, Nitrogen Oxides metabolism, Oxidative Stress drug effects, Rats, Rats, Inbred Dahl, Reactive Oxygen Species metabolism, Sodium Chloride, Transforming Growth Factor beta1 metabolism, p38 Mitogen-Activated Protein Kinases metabolism, Antioxidants pharmacology, Inflammation prevention & control, Kidney Diseases prevention & control, Kidney Tubules, Proximal pathology, Mesangial Cells pathology, Serpins pharmacology
- Abstract
An inverse relationship exists between kallistatin levels and salt-induced oxidative stress in Dahl-salt sensitive rats. We further investigated the role of kallistatin in inhibiting inflammation and fibrosis through antioxidative stress in Dahl-salt sensitive rats and cultured renal cells. High-salt intake in Dahl-salt sensitive rats induced elevation of thiobarbituric acid reactive substances (an indicator of lipid peroxidation), malondialdehyde levels, reduced nicotinamide-adenine dinucleotide phosphate oxidase activity, and superoxide formation, whereas kallistatin gene delivery significantly reduced these oxidative stress parameters. Kallistatin treatment improved renal function and reduced kidney damage as evidenced by diminished proteinuria and serum urea nitrogen levels, glomerular sclerosis, tubular damage, and protein cast formation. Kallistatin significantly decreased interstitial monocyte-macrophage infiltration and the expression of tumor necrosis factor-alpha, intercellular adhesion molecule-1, and vascular cell adhesion molecule-1. Kallistain also reduced collagen fraction volume and the deposition and expression of collagen types I and III. Renal protection by kallistatin was associated with increased NO levels and endothelial NO synthase expression and decreased p38 mitogen-activated protein kinase, extracellular signal-regulated kinase phosphorylation, and transforming growth factor-beta1 expression. Moreover, kallistatin attenuated tumor necrosis factor-alpha-induced intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 expression via inhibition of reactive oxygen species formation and p38 mitogen-activated protein kinase and nuclear factor-kappaB activation in cultured proximal tubular cells. Kallistatin inhibited fibronectin and collagen expression by suppressing angiotensin II-induced reactive oxygen species generation and transforming growth factor-beta1 expression in cultured mesangial cells. These combined findings reveal that kallistatin is a novel antioxidant, which prevents salt-induced kidney injury, inflammation, and fibrosis by inhibiting reactive oxygen species-induced proinflammatory cytokine and transforming growth factor-beta1 expression.
- Published
- 2008
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