1. International Laboratory Comparison of Influenza Microneutralization Assays for A(H1N1)pdm09, A(H3N2), and A(H5N1) Influenza Viruses by CONSISE
- Author
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Laurie, Karen L., Engelhardt, Othmar G., Wood, John, Heath, Alan, Katz, Jacqueline M., Peiris, Malik, Hoschler, Katja, Hungnes, Olav, Zhang, Wenqing, Van Kerkhove, Maria D., Katz, Jaccqueline, Xiuhua, Lu, Levine, Min, Veguilla, Vic, Liu, Feng, Bai, Yaohui, Puthavathana, Pilaipan, Lerdsamran, Hatairat, Pooruk, Phisanu, Nateerom, Knnika, Castrucci, Maria Rita, Donatelli, Isabella, Facchini, Marzia, Kishida, Noriko, Tashiro, Masato, Odagiri, Takato, Pawar, Shailesh D., Kode, Sadhana S., Hawksworth, Anthony, Ortiguerra, Ryan, Brice, Gary, Martin, Nicholas, Kochel, Tad, Sanchez, Jose, Cooper, Michael, Cummings, James, Wagner, Ralf, Goepfert, Constanze, Alex, Nina, Hammann, Joanna, Neumann, Britta, Perera, Mahendra, Montomoli, Emanuele, Lapini, Guilia, Sbragi, Sara, Bai, Tian, Zaijiang, Yu, Zhou, Jianfang, Carolan, Louise, and Laurie, Karen
- Subjects
Hemagglutination ,International Cooperation ,Clinical Biochemistry ,medicine.disease_cause ,Antibodies, Viral ,Serology ,Influenza A Virus, H1N1 Subtype ,REPRODUCIBILITY ,INFECTION ,Influenza A Virus ,Immunology and Allergy ,Viral ,SEROLOGIC ASSAYS ,biology ,H5N1 ,Titer ,Infectious Diseases ,1107 Immunology ,H5N1 Subtype ,Antibody ,Life Sciences & Biomedicine ,Human ,Microbiology (medical) ,Immunology ,Enzyme-Linked Immunosorbent Assay ,Microbiology ,Sensitivity and Specificity ,Virus ,Antibodies ,Orthomyxoviridae Infections ,Neutralization Tests ,Diagnostic Laboratory Immunology ,Influenza, Human ,medicine ,Microneutralization Assay ,Animals ,Humans ,H1N1 Subtype ,Protocol (science) ,Science & Technology ,Influenza A Virus, H5N1 Subtype ,Ferrets ,Reproducibility of Results ,Virology ,Influenza A virus subtype H5N1 ,Influenza ,ANTIBODY ,biology.protein ,CONSISE Laboratory Working Group participants - Abstract
The microneutralization assay is commonly used to detect antibodies to influenza virus, and multiple protocols are used worldwide. These protocols differ in the incubation time of the assay as well as in the order of specific steps, and even within protocols there are often further adjustments in individual laboratories. The impact these protocol variations have on influenza serology data is unclear. Thus, a laboratory comparison of the 2-day enzyme-linked immunosorbent assay (ELISA) and 3-day hemagglutination (HA) microneutralization (MN) protocols, using A(H1N1)pdm09, A(H3N2), and A(H5N1) viruses, was performed by the CONSISE Laboratory Working Group. Individual laboratories performed both assay protocols, on multiple occasions, using different serum panels. Thirteen laboratories from around the world participated. Within each laboratory, serum sample titers for the different assay protocols were compared between assays to determine the sensitivity of each assay and were compared between replicates to assess the reproducibility of each protocol for each laboratory. There was good correlation of the results obtained using the two assay protocols in most laboratories, indicating that these assays may be interchangeable for detecting antibodies to the influenza A viruses included in this study. Importantly, participating laboratories have aligned their methodologies to the CONSISE consensus 2-day ELISA and 3-day HA MN assay protocols to enable better correlation of these assays in the future.
- Published
- 2015