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• A simple way to upcycle wasted wool fabrics to WK/IL/PAN nanofibrous membrane. • Inhibiting 89.21% strains of E. coli , and 60.70% of S. aureus. • Performance of high moisture permeability. • IL can be recycled through distilled water, making the process sustainable. Wasted wool fabrics are a kind of textile waste source and the upcycle of them can not only benefit the environmental protection, but also turn waste into treasure by developing other potential applications. In this work, ionic liquid (IL) 1-butyl-3-methylimidazolium chloride ([Bmim]Cl) was used as a green solvent to upcycle wasted wool fabrics into a wool keratin (WK)/IL/polyacrylonitrile (PAN) composite nanofibrous membrane with good antibacterial and high moisture permeability through electrospinning. The morphology and structure of the regenerated nanofibrous membrane were characterized by Scanning Electronic Microscopy (SEM), Energy Dispersive Spectrometer (EDS), Fourier Transfer Infrared Spectroscopy (FTIR). The antibacterial test demonstrates that it has 89.21% inhibition rate against E. coli , and 60.70% against S. aureus. Furthermore, the keratin containing in the membrane can effectively improve the hydrophilic property of it, as Moisture Management Test (MMT) indicates that it performs an excellent wetting performance and water transport property. In addition, IL is supposed to be recycled from the composite membrane through immersing in distilled water, which makes the fabrication process green and sustainable. [ABSTRACT FROM AUTHOR]