1. Histidine tracts in human transcription factors: insight into metal ion coordination ability
- Author
-
Joanna Wątły, Henryk Kozlowski, Jolanta Świątek-Kozłowska, Magdalena Rowińska-Żyrek, and Aleksandra Hecel
- Subjects
0301 basic medicine ,inorganic chemicals ,Maf Transcription Factors, Large ,Stereochemistry ,Metal ions in aqueous solution ,Peptide ,Nerve Tissue Proteins ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Inorganic Chemistry ,Metal ,03 medical and health sciences ,chemistry.chemical_compound ,Coordination Complexes ,Amide ,Imidazole ,Homeostasis ,Humans ,Histidine ,Amino Acid Sequence ,Transcription factor ,Ligand binding ,chemistry.chemical_classification ,Original Paper ,Mass spectrometry ,Forkhead Transcription Factors ,Hydrogen-Ion Concentration ,Peptide Fragments ,0104 chemical sciences ,Zinc ,030104 developmental biology ,Binding affinity ,chemistry ,visual_art ,visual_art.visual_art_medium ,Thermodynamics ,Human genome ,Copper ,Protein Binding - Abstract
Consecutive histidine repeats are chosen both by nature and by molecular biologists due to their high affinity towards metal ions. Screening of the human genome showed that transcription factors are extremely rich in His tracts. In this work, we examine two of such His-rich regions from forkhead box and MAFA proteins—MB3 (contains 18 His) and MB6 (with 21 His residues), focusing on the affinity and binding modes of Cu2+ and Zn2+ towards the two His-rich regions. In the case of Zn2+ species, the availability of imidazole nitrogen donors enhances metal complex stability. Interestingly, an opposite tendency is observed for Cu2+ complexes at above physiological pH, in which amide nitrogens participate in binding. Electronic supplementary material The online version of this article (10.1007/s00775-017-1512-x) contains supplementary material, which is available to authorized users.
- Published
- 2017