Search

Bothriechis schlegelii is a venomous snake found in Central and South America, mainly sighted in regions devoted to agriculture. However, in Colombia, little is known about its contribution to the total envenoming cases. Furthermore, there are no reports of the biochemical and functional activities of venoms from the southwest populations, and the differences respecting other populations are unknown. This study analyzed the protein profiles of venom samples obtained from three specimens originating from this region of Colombia using electrophoresis and chromatography. The lethality, edema-induction, hemorrhagic, defibrinating, coagulant, and indirect hemolytic activities were also evaluated. As a result, venoms were composed of proteins with a wide range of molecular weights, most of them below37 kDa, with differences between male and female electrophoretic and chromatographic profiles. These variations were also observed in the evaluation of venom functional activities such as pro-coagulant, indirect hemolytic, and edema-inducing activities, whereas neither hemorrhagic nor defibrinating activities were detected. These results are also different considering reports with venom samples from other geographical locations, restating the existence of high intraspecific variability in B. schlegelii venoms, which could have relevant pathophysiological and therapeutic implications.

The systemic effects generated by Porthidium lansbergii lansbergii envenoming, a species found in the northern region of Colombia, is poorly known. The present study aimed to analyze for the first time the mice's behavior, the histological alterations, and changes in biochemical markers levels resulting from the intraperitoneal injection of an LD50 of P. lansbergii lansbergii snake venom on mice. The envenoming mice displayed hypodynamic condition, clonic head movements, accompanied by bradypnea and thoracoabdominal imbalance. After 7 h of envenoming, the mice showed an ecchymotic region at the injection site, including bleeding in the pleural, liver, and kidney capsules. The effect on the brain revealed a micro-hemorrhage in the sensorimotor cortex with substantial loss of neurons. The venom caused dilated blood vessels in lung tissue, with endothelial necrosis associated with alveolar rupture. The liver showed parenchyma alteration with many extravasated erythrocytes. The kidneys exhibited renal tubules necrosis and a statistically significant increase in creatinine concentration. ALP and ALT's enzymatic activities remained constant at 7 h after envenoming but increased at 12 h. AST and LDH were significantly increased at 7 h but decreased to the near baseline 12 h after venom administration. Massive hemorrhages could trigger a hypovolemic shock, which could lead to death after several h without treatment. Knowledge of P. lansbergii lansbergii snake bites' injuries is essential to make the appropriate diagnostic in human envenoming cases by this snake.