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The classification of insecticide resistance in sand flies populations is based on concepts and methodologies used to characterize the susceptibility profile in mosquitoes. This can generate erroneous and subjective interpretations since they are biologically different organisms. In this context, the goal of this review is to analyze the works and/or articles that aimed at characterizing the susceptibility of sand flies and describing the methodological parameters, in order to improve future works to estimate more accurately the resistance of sand flies to insecticides. Using keywords that refer to the purpose of this review, scientific studies in English, Spanish and Portuguese published until December 2019 were analyzed. A total of 3481 articles were found in searches in four databases (Pubmed, Scopus, BVS and ScienceDirect) and 61 were selected. The panorama of sand-fly resistance revealed 47 populations of sand flies, of species Phlebotomus papatasi, Ph. argentipes e Sergentomyia shorttii, with confirmed resistance, and 28 populations of species Ph. papatasi, Ph. argentipes, Ph. sergenti e Lutzomyia longipalpis. Of the 61 selected studies, only three studies performed comparisons between field and colony phlebotomines, and all colony populations were less susceptible than field populations to at least one tested insecticide. The lethal doses and lethal times of sand flies are very varied, revealing that there is no specific protocol for assessing the susceptibility of sand flies to insecticides. For a quick and early detection of sand flies' resistance to insecticides, we suggest the use of CDC bottle tests with an SRL to estimate the local Dose and Diagnostic Time. Males and females can be used in the same proportion, but with only female sand flies in the control group. Females with engorged abdomen or pregnant should be avoided in the experiment and, if possible, use the F1 generation of field sand flies, up to 5 days old, or at least 100 sand flies to reduce the influence of age on the susceptibility of the population., (Copyright © 2020. Published by Elsevier B.V.)

Background: Chemical mosquito control using malathion has been applied in Brazil since 1985. To obtain chemical control effectiveness, vector susceptibility insecticide monitoring is required. This study aimed to describe bioassay standardizations and determine the susceptibility profile of Ae. aegypti populations to malathion and pyriproxyfen, used on a national scale in Brazil between 2017 and 2018, and discuss the observed impacts in arbovirus control., Methods: The diagnostic-doses (DD) of pyriproxyfen and malathion were determined as the double of adult emergence inhibition (EI) and lethal doses for 99% of the Rockefeller reference strain, respectively. To monitor natural populations, sampling was performed in 132 Brazilian cities, using egg traps. Colonies were raised in the laboratory for one or two generations (F1 or F2) and submitted to susceptibility tests, where larvae were exposed to the pyriproxyfen DD (0.03 µg/l) and adults, to the malathion DD determined in the present study (20 µg), in addition to the one established by the World Health Organization (WHO) DD (50 µg) in a bottle assay. Dose-response (DR) bioassays with pyriproxyfen were performed on populations that did not achieve 98% EI in the DD assays., Results: Susceptibility alterations to pyriproxyfen were recorded in six (4.5%) Ae. aegypti populations from the states of Bahia and Ceará, with Resistance Ratios (RR 95 ) ranging from 1.51 to 3.58. Concerning malathion, 73 (55.3%) populations distributed throughout the country were resistant when exposed to the local DD 20 µg/bottle. On the other hand, no population was resistant, and only 10 (7.6%) populations in eight states were considered as exhibiting decreased susceptibility (mortality ratios between 90 and 98%) when exposed to the WHO DD (50 µg/bottle)., Conclusions: The feasibility of conducting an insecticide resistance monitoring action on a nation-wide scale was confirmed herein, employing standardized and strongly coordinated sampling methods and laboratory bioassays. Brazilian Ae. aegypti populations exhibiting decreased susceptibility to pyriproxyfen were identified. The local DD for malathion was more sensitive than the WHO DD for early decreased susceptibility detection.

Introduction: In Brasilia, pyriproxyfen (PPF; 0.01 mg/L) has been used for the larval control of Aedes aegypti mosquitoes since 2016. Information on the susceptibility of Ae. aegypti to PPF, and the development of resistance in populations from the Federal District of Brazil (FD) is limited. It is essential to monitor the susceptibility of Ae. aegypti to insecticides in order to improve vector control strategies. This study aimed to evaluate the susceptibility of Ae. aegypti populations from five areas of Brasilia to PPF., Methods: We performed dose-response tests to estimate the emergence inhibition and resistance ratio of each field population, including the Rockefeller reference population. We also analyzed egg positivity, and the density and mortality of larvae and pupae., Results: Populations from Vila Planalto (RR50=1.7), Regiment Guards Cavalry (RR50=2.5), and Sub-secretary of Justice Complex (RR50=3.7) presented high susceptibility to PPF, while the RR values of populations from Lago Norte (RR50=7.7) and Varjão (RR50=5.9) were moderately high, suggesting the emergence of insipient resistance to PPF in Brasilia. At 30 ng/mL, the highest larvae mortality rate was 2.7% for the population from Lago Norte, while that of pupae was 92.1% for Varjão and Vila Planalto., Conclusions: The five populations of Ae. aegypti from the FD are susceptible to PPF and there is a need to monitor the susceptibility of Ae. aegypti in new areas of the FD.

Introduction: Triatoma sordida Stål, 1859 populations were investigated for susceptibility to deltamethrin., Methods: This study aimed to analyze the resistance of 11 populations of insects from the States of Goiás, Mato Grosso and Mato Grosso do Sul by topical application bioassays., Results: The estimated LD50 and RR50 showed high levels of susceptibility (LD50 < 1 and RR50 < 2). However, analysis of the slope of the dose response curve showed that the populations of insects in the towns of Firminópolis/GO, Posse/GO, Poxoréu/MT, Douradina/MS and Aparecida do Taboado/MS present great probability of evolving resistance and, thus, are more likely to tolerate treatment with deltamethrin., Conclusions: Small changes in susceptibility and low levels of resistance were detected, but the temporal changes of susceptibility should be continuously monitored to adequately guide the actions of vector-control of DC.