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Many studies have tested the insulin absorption rate and mechanical reliability of jet injectors. However, no published papers have dealt exclusively with patient preference for this method of administering insulin over a period of years. This paper reports the results of a survey done to determine if use of a jet injector for delivering insulin has an effect on acceptance of and adherence to a regimen of multiple doses of insulin. Over the past 15 years, the authors have instructed approximately 70 patients to use various jet injectors. A questionnaire was developed and sent to 75 patients. Of these, 42 completed and returned the questionnaire. Survey results indicate that even though some problems with the injectors were identified, 70% of those responding still preferred to take insulin by jet injector.

Primary monolayer cultures of human B cells established using collagenase digestion, assume a characteristic epithelial morphology which is ideal for indirect immunofluorescence studies. Using double label indirect immunofluorescence, it was possible to identify an extensive cytoplasmic microtubule complex and numerous insulin antigen positive granules within the human B cells. The microtubules are discrete rod-like structures which terminate at, or near, the plasma membrane and are observed sometimes in close association with insulin antigen positive granules. This culture system is well-suited for studying the role of the microtubules in human B cell function.