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Start Over Descriptor "Insulin-Like Growth Factor Binding Protein 6 biosynthesis" Topic insulin-like growth factor binding protein 4
2 results on '"Insulin-Like Growth Factor Binding Protein 6 biosynthesis"'

1. Insulin-like growth factor-binding protein (IGFBP)-1, -2, -3, -4, -5, and -6 and IGFBP-related protein 1 during rainbow trout postvitellogenesis and oocyte maturation: molecular characterization, expression profiles, and hormonal regulation.

Author

Kamangar BB, Gabillard JC, and Bobe J

Subjects
Amino Acid Sequence, Animals, Cloning, Molecular, DNA, Complementary metabolism, Estradiol metabolism, Expressed Sequence Tags, Female, Hormones metabolism, Liver metabolism, Male, Molecular Sequence Data, Oncorhynchus mykiss, Ovary metabolism, Phylogeny, RNA metabolism, RNA, Messenger metabolism, Reverse Transcriptase Polymerase Chain Reaction, Sequence Homology, Amino Acid, Tissue Distribution, Gene Expression Regulation, Developmental, Insulin-Like Growth Factor Binding Protein 1 biosynthesis, Insulin-Like Growth Factor Binding Protein 2 biosynthesis, Insulin-Like Growth Factor Binding Protein 3 biosynthesis, Insulin-Like Growth Factor Binding Protein 4 biosynthesis, Insulin-Like Growth Factor Binding Protein 5 biosynthesis, Insulin-Like Growth Factor Binding Protein 6 biosynthesis, Insulin-Like Growth Factor Binding Proteins biosynthesis, Oocytes metabolism, Vitellogenesis
Abstract

In the present study we report the full coding sequence of rainbow trout IGF-binding protein-1 (IGFBP1), -2, -3, -5, and -6 and IGFBP-related protein-1 (IGFBP-rP1) mRNAs as well as the partial coding sequence of IGFBP-4 mRNA. To our knowledge, this is the first report of IGFBP4, IGFBP6, and IGFBP-rP1 in a nonmammalian species. The tissue distribution of all mRNAs was studied, and the ovarian expression profiles of IGFBP2 to -6 and IGFBP-rP1 between late vitellogenesis and oocyte maturation were characterized. In addition, in vitro hormonal regulation by the maturation-inducing steroid 17,20beta-dihydroxy-4-pregnen-3-one (17,20betaP), gonadotropin, and estradiol were studied. We observed that besides IGFBP1, which was only found in liver, IGFBP2 to -6 and IGFPB-rP1 were expressed in the preovulatory ovary. IGFBP3 was also detected in liver, trunk, kidney, skin, and gills, whereas IGFBP2 to -6 and IGFBP-rP1 exhibited a wider tissue distribution. In the preovulatory ovary, IGFBP3 was strongly down-regulated during the postvitellogenesis period, whereas IGFBP5 exhibited a limited up-regulation. In addition, IGFBP6 and IGFBP-rP1 were up-regulated during oocyte maturation. Hormonal regulation data indicated that all ovarian IGFBPs and IGFBP-rP1 transcripts are regulated under gonadotropic stimulation at a concentration that induced 100% oocyte maturation. In addition, IGFBP2 to -5 transcripts are regulated by 17,20betaP and estradiol. Together, our observations strongly suggest that during final oocyte maturation, a down-regulation of IGFBP3, -4, and -5 occurs in the oocyte in response to gonadotropic and 17,20betaP (IGFBP3 and -5) stimulation, whereas an up-regulation of IGFBP2 and -6 occurs in follicular layers or extrafollicular tissue in response to gonadotropic stimulation.

Published
2006
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2. Inhibition of growth and increased expression of insulin-like growth factor-binding protein-3 (IGFBP-3) and -6 in prostate cancer cells stably transfected with antisense IGFBP-4 complementary deoxyribonucleic acid.

Author

Drivdahl RH, Sprenger C, Trimm K, and Plymate SR

Subjects
Animals, Apoptosis, Cell Division, Humans, Insulin-Like Growth Factor Binding Protein 4 antagonists & inhibitors, Male, Mice, Mice, Nude, Prostatic Neoplasms metabolism, Prostatic Neoplasms pathology, Transfection, Tumor Cells, Cultured, DNA, Antisense therapeutic use, Insulin-Like Growth Factor Binding Protein 3 biosynthesis, Insulin-Like Growth Factor Binding Protein 4 physiology, Insulin-Like Growth Factor Binding Protein 6 biosynthesis, Prostatic Neoplasms therapy
Abstract

Insulin-like growth factor-binding proteins (IGFBPs) both stimulate and inhibit IGF activity, and in the M12 prostate cancer cell line, overexpression of IGFBP-4 was shown to delay tumorigenesis while decreasing the production of IGFBP-2. We have performed the reverse experiment, inhibition of IGFBP-4 expression with antisense complementary DNA, in two prostate tumor cell lines, ALVA-31 and M12. Expression of antisense messenger RNA transcripts was verified by RNase protection assays, and inhibition of mature IGFBP-4 in cell medium was demonstrated by Western blotting. Both transfected lines (ALVA-31asBP4 and M12asBP4) proliferated more slowly in monolayer culture than parental controls. Colony formation in soft agar was strongly inhibited in both cases, and the rate of tumor formation and growth in male athymic nude mice injected with M12asBP4 was markedly reduced relative to that in mice receiving M12 control cells. Apoptosis induced by the topoisomerase inhibitor etoposide was also enhanced in transfected cells. The effects on colony formation in soft agar and tumor formation in mice were maintained for the duration of the experiments, in contrast to the delayed growth observed in the previous study of IGFBP-4 overexpression. A significant difference was found in the patterns of IGFBP expression; production of both messenger RNA and protein for IGFBP-3 and IGFBP-6 was greatly increased in the M12asBP4 and ALVA31asBP4 cell lines. Up-regulation of these binding proteins has been observed in association with actions of 1,25-dihydroxyvitamin D(3) in prostate cancer cells, and the data suggest a role for IGFBP-3 and IGFBP-6 in the suppression of prostate tumor cell growth.

Published
2001
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