1. Interleukin-4 suppresses granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor levels in stimulated human monocytes.
- Author
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Hamilton, J. A., Whitty, G. A., Royston, A. K. M., Cebon, J., and Layton, J. E.
- Subjects
INTERLEUKIN-4 ,GRANULOCYTES ,MACROPHAGES ,MONOCYTES ,INTERFERONS ,ANTIVIRAL agents - Abstract
Granulocyte colony-stimulating factor (G-CSF) was quantitated in the supernatants of lipopolysaccharide (LPS)-treated human monocytes by ELISA. Unlike previous reports, the lymphokines, interleukin-4 (IL-4) and interferon-γ (IFN-γ), were unable to induce the synthesis of G-CSF. Both IL-4 (⩾ 10 pM) and the glucocorticoid, dexamethasone (10
-7 M), inhibited G-CSF production in the LPS-treated monocytes; in contrast, IFN-γ had a weak potentiating effect on the LPS action. Changes in antigen expression were manifested at the level of messenger RNA (mRNA). Granulocyte-macrophage (GM)-CSF in the LPS-treated monocyte supernatants was also quantitated by ELISA but its levels were somewhat lower than for G-CSF; IL-4, dexamethasone and IFN-γ had similar effects on GM-CSF levels as on G-CSF levels. The suppression of CSF production in the stimulated monocytes by IL-4 and glucocorticoid extends the list of monocyte cytokines whose levels can be down-regulated by these agents and suggests another potential anti-inflammatory and immunosuppressive function for IL-4. [ABSTRACT FROM AUTHOR]- Published
- 1992