1. Suppression of IL-17F, but not of IL-17A, provides protection against colitis by inducing T reg cells through modification of the intestinal microbiota.
- Author
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Tang C, Kakuta S, Shimizu K, Kadoki M, Kamiya T, Shimazu T, Kubo S, Saijo S, Ishigame H, Nakae S, and Iwakura Y
- Subjects
- Animals, Cells, Cultured, Clostridium growth & development, Clostridium isolation & purification, Colitis drug therapy, Interleukin-17 genetics, Interleukin-17 physiology, Intestines immunology, Mice, Mice, Inbred C57BL, Mice, Knockout, Phospholipases A2 biosynthesis, Phospholipases A2 genetics, Prevotella isolation & purification, Ribonuclease, Pancreatic biosynthesis, Ribonuclease, Pancreatic genetics, beta-Defensins biosynthesis, Colitis immunology, Gastrointestinal Microbiome, Interleukin-17 antagonists & inhibitors, T-Lymphocytes, Regulatory immunology
- Abstract
The cytokines IL-17A and IL-17F have 50% amino-acid identity and bind the same receptor; however, their functional differences have remained obscure. Here we found that Il17f
-/- mice resisted chemically induced colitis, but Il17a-/- mice did not, and that Il17f-/- CD45RBhi CD4+ T cells induced milder colitis in lymphocyte-deficient Rag2-/- mice, accompanied by an increase in intestinal regulatory T cells (Treg cells). Clostridium cluster XIVa in colonic microbiota capable of inducing Treg cells was increased in both Il17f-/- mice and mice given transfer Il17f-/- T cells, due to decreased expression of a group of antimicrobial proteins. There was substantial production of IL-17F, but not of IL-17A, not only by naive T cells but also by various colon-resident cells under physiological conditions. Furthermore, antibody to IL-17F suppressed the development of colitis, but antibody to IL-17A did not. These observations suggest that IL-17F is an effective target for the treatment of colitis.- Published
- 2018
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