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Lipids are a major component of the cell membrane, essential for the growth and division of both the normal and malignant cell. This study aimed to study the effect of different chemotherapy regimens in the induction of dyslipidemia in Iraqi women with breast cancer. This cross-sectional study was conducted at the Department of Biochemistry, Medical College, University of Baghdad and at the Oncology Hospital, Medical City Hospital, Baghdad, Iraq, during the period from May 2018 to October 2018. It involved 56 regularly menstruated women (25-45 years) categorized into: group 1 [G1] included 29 women with primary breast carcinoma, group 2 [G2] consisted of the same 29 women of G1 but after finishing the first course of treatment [4 cycles of anthracycline chemotherapy], and group 3 [G3] which involved 27 women who completed full regimen of chemotherapy treatment [course 1 and 4 cycles of taxanes; the course 2]. Serum cholesterol, LDL-cholesterol (LDL-C), HDL-cholesterol (HDL-C) and TG were measured using colorimetric methods. The mean (± SEM) of serum cholesterol was significantly increased in G1 (p< 0.05) and G2 (p

Heart-type fatty acid binding protein (H-FABP) also referred to as mammary­ derived growth inhibitor is a polypeptide structure that in humans transcribed by FABP3 gene. It is preferred to be investigated in combination with troponin to the diagnosis of myocardial infarction in patients suffering from chest pain. This study aims to evaluate the role of H­ FABP in early diagnosis of MI in comparison with new generation cardiac troponin (hs-c'Tn) and to differentiate patients with ischemic chest pain from non-ischemic ones. This case-control study was performed at the Department of Biochemistry, Medical school, University of Baghdad, during the period from December 2017 to August 2018. It involved 36 patients presented with chest pain; 18 ischemic patients (AMI) and 18 non- ischemic patients (non-AMI) who served as pathological control. Serum investigations included measurements of FABP and hs-c'I'n using enzyme-linked immunosorbent assay (ELISA) method. The mean (±SD) value of serum FABP levels at 1-3 hours did not differ significantly between ischemic and non- ischemic subjects, while it was significantly increased at 6-9 hours in ischemic patients (p< 0.001). However, the mean value of serum levels of hs-c'I'n was significantly higher in AMI patients than in non- AMI ones at 1-3 hours (p

Background: Lipids seem to have a direct influence in women breast cancer (BC) or dyslipidemia may be a consequence of this cancer. Objective: the aim of this study is to define serum levels of lipid profile in women with breast cancer (BC) and to compare that with fibroadenoma benign breast tumor. Also, to assess the role of serum CA 15-3 in early diagnosis of breast cancer (BC). Patients and Methods: This case study was carried out at Biochemistry Department, College of Medicine, University of Baghdad, during the period from February 2017 to November 2017. It included 38 Iraqi women diagnosed with primary breast cancer (BC, Group-I) and 25 women with Fibroadenoma of benign breast tumor (FA, Group-II). Women with BC were sub divided according to their stage of BC into: stage I (n=12), stage II (n=14), and stage III (n=12). Investigations included serum measurements of CA 15-3 and lipid profile (Total Cholesterol, Triglyceride, LDL-Cholesterol and HDL-Cholesterol). Results: The mean value of CA15-3 in breast cancer women was found to be significantly higher (p

Background: Vitamin D and Leptin appears to play a range of roles in beta cell growth and insulin secretion and most importantly interaction with other hormonal mediators and regulators of energy and metabolism. Objective: The aim of this study was to measure serum leptin and vitamin D levels and to investigate their relationships with vitamin D and other clinical laboratory parameters in patients with type II diabetes. Subjects and Methods: Blood samples were taken from 80 patients with type II diabetes mellitus encountered during their attending the Internal Medicine clinic consultancy in Ramadi Teaching Hospital and the National Diabetes Center for Treatment and Research at Al-Mustansiriya University and 60 healthy subject. From December 2014 to November 2015. Investigations included serum Leptin, 25OHD, Insulin, HbA1c using ELISA and biochemical test.Results: The median concentration of serum 25 OH vitamin D of patients (15.70 ng/ ml) was significantly lower than healthy controls (20.27 ng/ ml). The rate of vitamin D deficiency (VDD) was significantly higher in patients (82.5%) than healthy controls (48.3%). The serum insulin and HOMA-IR were significant increase in patients had vitamin D < 20 ng/ml when compared with an insufficient/normal group. There were no significant differences in leptin levels between type II DM and healthy control. Conclusion: These results strongly support the role of vitamin D deficiency and serum leptin in pathogenesis of type II diabetes.

Objectives To investigate the role of serum anti mullerian hormone (AMH) pre-chemotherapy treatment levels in prediction of post-chemotherapy effect on the ovarian reserve of women with breast cancer. Methods This cohort prospective study was carried out at the Biochemistry Department, College of Medicine, University of Baghdad and at the Oncology Clinic, Oncology Teaching Hospital, Baghdad, Iraq. It included 58 women with regular menstrual cycle (25-45 years) who were newly diagnosed with breast cancer. The women were classified into 3 groups: GI: 30 women with breast cancer before starting chemotherapy, GII: the same 30 women of GI who finished 4 cycles of anthracycline chemotherapy (course 1) and GIII: which involved another 28 women who had finished both courses of chemotherapy, (course 1) and (course 2). Results The mean (±SEM) value of AMH levels was significantly decreased in GII and GIII when compared with GI (for both, p less than 0.0005). However, there was no significant difference in serum AMH levels between GII and GIII. Conclusion The measurement of serum AMH may be a useful biochemical marker of the chemotherapy extent induced ovarian reserve damage and the incidence of amenorrhea.

Objectives: To evaluate the role of anti-Mullerian hormone (AMH) and inhibin B in the evaluation of the effectiveness of short- (3 months) and long-term (6 months or more) metformin therapy in Iraqi women with polycystic ovarian syndrome (PCOS). Methods: This cross-sectional study was carried out at the Biochemistry Department, College of Medicine, University of Baghdad, Baghdad, Iraq from June 2010 to May 2011. It included 38 volunteers of women patients with PCOS, aged 18-38 years, who were classified into: Group I (GI, n=20); Group II included women in GI that were followed up after they were treated with metformin hydrochloride tablet 500 mg 3 times daily for 3 months; and GIII included 18 women that were already on metformin hydrochloride treatment 500 mg tablet 3 times daily for 6 months to 3 years. Investigations included serum measurement of insulin, AMH, inhibin B, androgen hormones using enzyme-linked immunosorbent assay, and mini Vidus techniques. Results: The mean ± standard error of the mean value of serum AMH levels was significantly decreased in post metformin treatment women (3 months; GII) compared with those before treatment (GI), and those women on prolonged treatment (GIII) ( p

Slothful life style and lack of regular physical activity are the main causes of obesity, obesity- induced metabolic disorders. The aims of this study were to consolidation the harmony of serum Irisin with rats' fat development. Thinking over the innovation, can Irisin be therapeutic for metabolic disorders without regular exercise. This prospective experimental study was included 120 Western rats. The study had undergo two lines first was the correction of inducible obesity & diabetes; second was the prevention of mentioned inducible conditions, both lines was done by subjecting the rats to subcutaneous injection of prepared Irisin solution (100 ng/ml). Inducible obese type 2 diabetic (T2DM) rats had significantly higher serum Irisin, insulin level & fasting glucose (S.F.G.) in comparison with inducible obese rats & normal weight rats. Obese rats that were injected with 100 ng/ml of recombinant Irisin had highly significant lower weight mean and S.F.G. than obese rats control . Established diabetic rats with Irisin injection was significantly corrected, with their weight , serum glucose , and insulin after 26 days of irisin treatment. Findings showed that Irisin may act as reformation effect on new onset type 2 diabetics' rats; as well as it may have intensive defiance against fatness . Keywords -Irisin, Obese Rats, Insulin Resistance ,Obesity Treatment Diabetic Rats, Rat Obesity Induction.

Background: Recently on the dermatological fields, the serum levels and the roles of Zn, Cu and Mg have been studied especially in acne vulgaris, but the results were controversial. Objective: The aim of the present study is to investigate a relationship between the severity of acne and the serum levels of zinc (Zn), copper (Cu) and magnesium (Mg) in Iraqi males. Subjects and Methods: This case controlled study was conducted in the Department of Biochemistry, College of Medicine, Baghdad University, in the Dermatology and Venereology and in the Poisoning Consultation Center of Baghdad Teaching Hospital between May 2009 to January 2010. Forty- five male patients with acne vulgaris, their ages ranged from 18-30 [21.82 ± 3.77, as (mean ± SD)] years and 45 healthy male subjects as controls, their ages ranged from 18-30 (22.18 ± 3.85) years were included in this study. Patients were subdivided into three groups according to the severity of their acne; mild acne group (n=15), moderate (n=15) and severe acne group (n=15). Investigations included serum estimation of Zn, Cu and Mg in both patient and control groups. Results: The (mean ± SD) values of serum levels of Zn, Cu and Mg did not differ significantly between the patient [all severities combined] and control groups. However, serum Zn level was significantly decreased in severe acne group compared with controls (P=0.0001), mild-(P=0.0001) and moderate-(P=0.003) acne groups. Serum Mg concentration was significantly lower in severe acne group compared with mild acne group (P