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7 results on '"Biofysisch"'

1. Tumor cell killing by in vitro affinity-matured recombinant human monoclonal antibodies

Author

Huls, G., Gestel, D., van der Linden, J., Moret, E.E., Logtenberg, T., Biofysisch, Medicinal Chemistry van immunologische en neurologische ziekteprocessen, Universiteit Utrecht, and Dep Farmaceutische wetenschappen

Subjects
Farmacie/Biofarmaceutische wetenschappen (FARM), Econometric and Statistical Methods: General, Geneeskunde (GENK), International, Immunology, Biophysics, Farmacie(FARM), Nutrition sciences, Farmaceutische produkten, Bescherming en bevordering van de menselijke gezondheid, Geneeskunde(GENK), Occupational medicine, Biomedische technologie en medicijnen
Published
2001

2. Exploring computational lead optimisation with affinity constants obtained by surface plasmon resonance for the interaction of PorA epitope peptides with antibody against Neisseira meningitidis

Author

Fischer, M.J.E., Kuipers, C., Hofkes, R.P., Hofmeyer, L.J.F., Moret, E.E., de Mol, N.J., Biofysisch, Medicinal Chemistry van immunologische en neurologische ziekteprocessen, SYNTHESE, Universiteit Utrecht, and Dep Farmaceutische wetenschappen

Subjects
Farmacie/Biofarmaceutische wetenschappen (FARM), International, Biophysics, Farmacie(FARM), Nutrition sciences, Farmaceutische produkten, Occupational medicine, Biomedische technologie en medicijnen
Published
2001

3. Structure-based design and evaluation of MHC class II binding peptides

Author

de Haan, E.C., Wauben, M.H.M., Grosfeld-Stulemeyer, M.C., Moret, E.E., Biofysisch, Medicinal Chemistry van immunologische en neurologische ziekteprocessen, Universiteit Utrecht, Dep Farmaceutische wetenschappen, and Faculteit Diergeneeskunde

Subjects
Farmacie/Biofarmaceutische wetenschappen (FARM), International, Biophysics, Farmacie(FARM), Nutrition sciences, Farmaceutische produkten, Diergeneeskunde (DGNK), Occupational medicine, Biomedische technologie en medicijnen
Published
2001

4. Scoring peptide(mimetic)-protein interactions

Author

Moret, E.E., van Wijk, M.C., Kostense, A.S., Gillies, M.B., Biofysisch, Universiteit Utrecht, and Dep Farmaceutische wetenschappen

Subjects
International, Farmacie(FARM)
Published
1999

5. Structure-based design and evaluation of MHC class II binding peptides

Author

de Haan, E.C., Wauben, M.H.M., Grosfeld-Stulemeyer, M.C., Moret, E.E., Biofysisch, Medicinal Chemistry van immunologische en neurologische ziekteprocessen, Universiteit Utrecht, Dep Farmaceutische wetenschappen, and Faculteit Diergeneeskunde

Subjects
Farmacie/Biofarmaceutische wetenschappen (FARM), International, Biophysics, Farmacie(FARM), Nutrition sciences, Farmaceutische produkten, Diergeneeskunde (DGNK), Occupational medicine, Biomedische technologie en medicijnen
Published
2001

6. Tumor cell killing by in vitro affinity-matured recombinant human monoclonal antibodies

Author

G. Huls, D. Gestel, J. van der Linden, Ed E. Moret, T. Logtenberg, Biofysisch, Medicinal Chemistry van immunologische en neurologische ziekteprocessen, Universiteit Utrecht, and Dep Farmaceutische wetenschappen

Subjects
Models, Molecular, Cancer Research, Phage display, Biomedische technologie en medicijnen, law.invention, Farmacie/Biofarmaceutische wetenschappen (FARM), Mice, law, Tumor Cells, Cultured, Immunology and Allergy, Microscopy, Confocal, Geneeskunde (GENK), Farmacie(FARM), Antibodies, Monoclonal, DNA shuffling, Eukaryotic Cells, Oncology, International, Colonic Neoplasms, Recombinant DNA, Nutrition sciences, Electrophoresis, Polyacrylamide Gel, Farmaceutische produkten, Antibody, medicine.drug_class, Molecular Sequence Data, Immunology, Biophysics, Biology, Monoclonal antibody, Immunoglobulin light chain, Cell Line, Affinity maturation, Antigens, Neoplasm, Peptide Library, medicine, Animals, Humans, Amino Acid Sequence, Bescherming en bevordering van de menselijke gezondheid, Geneeskunde(GENK), Panning (camera), Dose-Response Relationship, Drug, Sequence Homology, Amino Acid, Complement System Proteins, Molecular biology, General [Econometric and Statistical Methods], Kinetics, biology.protein, Mutagenesis, Site-Directed, Immunoglobulin Light Chains, Occupational medicine
Abstract

We have developed a method that allows the rapid improvement of the affinity of phage-displayed antibody fragments by selection on intact eukaryotic cells. A single chain Fv fragment, specific for the tumor-associated Ep-Cam molecule, was mutagenized by shuffling of the immunoglobulin light chain variable region and DNA shuffling of both heavy and light chain variable regions. Higher-affinity mutants were selected from small phage display libraries by cell panning under stringent conditions. When converted to an intact fully human antibody, the mutagenized anti-tumor monoclonal antibody displayed an affinity of 0.4 nM, a 15-fold improvement over the affinity of the original antibody. Compared to previously reported affinity maturation schemes, panning on intact cells does not require purified targets for selection and may be particularly useful when the target molecule can not be expressed as a recombinant molecule or easily purified without disrupting its native configuration. In vitro tumor cell killing assays demonstrated an improved performance of the higher-affinity antibody in complement-mediated tumor cell killing. In contrast, the lower-affinity antibody performed somewhat better in antibody-dependent cellular cytotoxicity assays and penetrated better in multicell spheroids of tumor cells, an in vitro model for the tumor penetration capacity of antibodies.

Published
2001

7. Scoring peptide(mimetic)-protein interactions

Author

Moret, E. E., Wijk, M. C., Kostense, A. S., Malcolm Gillies, Biofysisch, Universiteit Utrecht, and Dep Farmaceutische wetenschappen

Subjects
International, Farmacie(FARM)

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