1. A WiFi scanner in conjunction with disposable multiplex paper assay for the quantitation of disease markers in blood plasma.
- Author
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Hou P, Deng R, Guo J, Chen W, Li X, and Yu HZ
- Subjects
- Biomarkers blood, Colorimetry instrumentation, Humans, Point-of-Care Systems, Reproducibility of Results, Risk Factors, Smartphone, Wireless Technology, Blood Glucose analysis, Internet, Paper, Triglycerides blood, Uric Acid blood
- Abstract
Herein we report a quantitative, multiplex assay for disease markers in plasma based on an integrated setup of a portable scanner and a disposable paper-based analytical device (PAD). The quantitative analysis relies on the digital colorimetric reading of the three-layer PAD with 30 assay sites for performing respective chromogenic reactions for plasma uric acid, glucose, and triglyceride, which are considered as important risk factors for cardiovascular diseases. A portable scanner with WiFi transmission capability was used to produce high-quality color images of the PADs and wirelessly transfer them to a smartphone or other mobile devices for data processing. The concentrations of biomarkers in both standard solutions and plasma samples can be directly obtained using a custom-designed smartphone app that is also capable of constructing calibration curves. The detection limits of uric acid, glucose, and triglyceride were determined to be 0.50 mg/dL, 0.84 mmol/L, and 14 mg/dL, respectively, which are below the normal limits and adequate for clinical validation. Owing to the distinct advantages-simple, portable, and cost-effective-this mobile assay protocol can be used for point-of-care (POC) settings or resource-limited situations, and potentially for the diagnosis and prevention of infectious diseases.
- Published
- 2021
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