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Your search keyword '"Fre, S."' showing total 4 results
Start Over Author "Fre, S." Topic intestines
4 results on '"Fre, S."'

1. Notch signaling in intestinal homeostasis across species: the cases of Drosophila, Zebrafish and the mouse.

Author

Fre S, Bardin A, Robine S, and Louvard D

Subjects
Animals, Carcinoma genetics, Carcinoma metabolism, Carcinoma pathology, Homeostasis physiology, Humans, Intestinal Mucosa metabolism, Intestinal Neoplasms genetics, Intestinal Neoplasms metabolism, Intestinal Neoplasms pathology, Receptors, Notch genetics, Receptors, Notch metabolism, Signal Transduction genetics, Signal Transduction physiology, Species Specificity, Drosophila genetics, Drosophila metabolism, Drosophila physiology, Homeostasis genetics, Intestines physiology, Mice genetics, Mice metabolism, Mice physiology, Receptors, Notch physiology, Zebrafish genetics, Zebrafish metabolism, Zebrafish physiology
Abstract

Notch signaling has been recently shown to have a fundamental role in stem cell maintenance and control of proper homeostasis in the intestine of different species. Here, we briefly review the current literature on Notch signals in the intestine of Drosophila, Zebrafish and the mouse, and try to highlight conserved and divergent Notch functions across species. Notch signals show a remarkably conserved role in skewing cell fate choices in intestinal lineages throughout evolution. Genetic analysis demonstrates that loss of Notch signaling invariably leads to increased numbers of secretory cells and loss of enterocytes, while gain of Notch function will completely block secretory cell differentiation. Finally, we discuss the potential contribution of Notch signaling to the initiation of colorectal cancer by controlling the maintenance of the undifferentiated state of intestinal neoplastic cells and speculate on the therapeutic consequences of affecting cancer stem cells., (Copyright © 2011 Elsevier Inc. All rights reserved.)

Published
2011
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2. Notch lineages and activity in intestinal stem cells determined by a new set of knock-in mice.

Author

Fre S, Hannezo E, Sale S, Huyghe M, Lafkas D, Kissel H, Louvi A, Greve J, Louvard D, and Artavanis-Tsakonas S

Subjects
Animals, Basic Helix-Loop-Helix Transcription Factors genetics, Basic Helix-Loop-Helix Transcription Factors metabolism, Cell Differentiation, Clone Cells, Enterocytes cytology, Enterocytes metabolism, Gene Targeting, Homeodomain Proteins genetics, Homeodomain Proteins metabolism, Integrases metabolism, Kinetics, Mice, Mice, Inbred C57BL, Mice, Transgenic, Microvilli metabolism, Multipotent Stem Cells cytology, Multipotent Stem Cells metabolism, Sequence Homology, Amino Acid, Signal Transduction, Transcription Factor HES-1, Transcription, Genetic, Cell Lineage, Gene Knock-In Techniques, Intestines cytology, Receptors, Notch metabolism, Stem Cells cytology, Stem Cells metabolism
Abstract

The conserved role of Notch signaling in controlling intestinal cell fate specification and homeostasis has been extensively studied. Nevertheless, the precise identity of the cells in which Notch signaling is active and the role of different Notch receptor paralogues in the intestine remain ambiguous, due to the lack of reliable tools to investigate Notch expression and function in vivo. We generated a new series of transgenic mice that allowed us, by lineage analysis, to formally prove that Notch1 and Notch2 are specifically expressed in crypt stem cells. In addition, a novel Notch reporter mouse, Hes1-EmGFP(SAT), demonstrated exclusive Notch activity in crypt stem cells and absorptive progenitors. This roster of knock-in and reporter mice represents a valuable resource to functionally explore the Notch pathway in vivo in virtually all tissues.

Published
2011
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4. Notch signals control the fate of immature progenitor cells in the intestine.

Author

Fre S, Huyghe M, Mourikis P, Robine S, Louvard D, and Artavanis-Tsakonas S

Subjects
Animals, Apoptosis, Basic Helix-Loop-Helix Transcription Factors, Cell Differentiation, Cell Proliferation, DNA-Binding Proteins genetics, Epithelial Cells cytology, Epithelial Cells metabolism, Female, Gene Expression Regulation, Homeodomain Proteins genetics, Homeodomain Proteins metabolism, Integrases genetics, Integrases metabolism, Male, Mice, Mice, Transgenic, Nerve Tissue Proteins genetics, RNA, Messenger genetics, RNA, Messenger metabolism, Receptor, Notch1, Receptors, Cell Surface genetics, Transcription Factor HES-1, Transcription Factors genetics, Viral Proteins genetics, Viral Proteins metabolism, Cell Lineage, Intestinal Mucosa metabolism, Intestines cytology, Receptors, Cell Surface metabolism, Signal Transduction, Stem Cells cytology, Stem Cells metabolism, Transcription Factors metabolism
Abstract

The Notch signalling pathway plays a crucial role in specifying cellular fates in metazoan development by regulating communication between adjacent cells. Correlative studies suggested an involvement of Notch in intestinal development. Here, by modulating Notch activity in the mouse intestine, we directly implicate Notch signals in intestinal cell lineage specification. We also show that Notch activation is capable of amplifying the intestinal progenitor pool while inhibiting cell differentiation. We conclude that Notch activity is required for the maintenance of proliferating crypt cells in the intestinal epithelium.

Published
2005
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