1. Ion-transfer voltammetry of streptothricin antibiotics with differently sized lysine oligomers at a nitrobenzene | water interface.
- Author
-
Uematsu, Kohei, Maruyama, Chitose, Hamano, Yoshimitsu, and Katano, Hajime
- Subjects
- *
STREPTOTHRICIN , *VOLTAMMETRY , *ANTIBIOTICS , *LYSINE , *OLIGOMERS , *NITROBENZENE , *WATER - Abstract
The transfer of streptothricin antibiotics containing a β-lysine monomer (ST-F), trimer (ST-D), and tetramer (ST-C) at a polarizable nitrobenzene (NB) | water (W) interface was studied by cyclic voltammetry. Under acidic conditions, the streptothricins existed in fully protonated forms, as ST-F 2 + , ST-D 4 + and ST-C 5 + cationic species. Within the potential range tested, ST-D 4 + and ST-C 5 + ions gave reversible voltammograms due to their transfer across the NB | W interface. The formal potentials, Δ O W ϕ 0 S ’ T , of ST-D 4 + and ST-C 5 + ions were determined. In the presence of a neutral ligand (L), dibenzo-18-crown-6, in NB, ST-F 2 + , ST-D 4 + and ST-C 5 + ions gave reversible voltammograms due to their transfer assisted by the formation of ST-FL 2 2 + , ST-DL 4 4 + and ST-CL 5 5 + complexes with the ligand in NB. Using the Δ O W ϕ 0 S ’ T -values, association constants between the NH 3 + -groups of ST-D 4 + and ST-C 5 + ions and the crown ether in NB were determined. The association constants were in agreement with each other. By assuming that the association constant of ST-F 2 + was equal to those of the ST-D 4 + and ST-C 5 + ions, the Δ O W ϕ 0 S ’ T of the ST-F 2 + ion was estimated. Interestingly, the determined Δ O W ϕ 0 S ’ T -value was increasingly negative in the order of ST-F 2 + (~ 0.24 V) > ST-D 4 + (0.199 V) > ST-C 5 + (0.194 V), indicating that the lysine oligomer affected the phase-transfer characteristics of streptothricin. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF