1. Core-Corona Magnetic Nanospheres Functionalized with Zwitterionic Polymer Ionic Liquid for Highly Selective Isolation of Glycoprotein.
- Author
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Guo ZY, Hai X, Wang YT, Shu Y, Chen XW, and Wang JH
- Subjects
- Adsorption, Chromatography, Liquid methods, Electrophoresis, Polyacrylamide Gel, Esterification, Glycoproteins chemistry, Humans, Immunoglobulin G blood, Immunoglobulin G isolation & purification, Immunoglobulin G metabolism, Microscopy, Electron, Transmission, Spectrometry, X-Ray Emission, X-Ray Diffraction, Ferrosoferric Oxide chemistry, Glycoproteins isolation & purification, Ionic Liquids chemistry, Magnetite Nanoparticles chemistry, Nanospheres chemistry, Polyesters chemistry
- Abstract
A novel zwitterionic polymer ionic liquid functionalized magnetic nanospheres, shortly as Fe
3 O4 @PCL-PILs, is synthesized by grafting ionic liquid VimCOOHBr onto polymer ε-caprolactone (PCL) modified magnetic nanospheres via esterification and surface-initiated free radical polymerization. This established synthesis strategy offers the obtained magnetic nanospheres with well-defined core-corona structure, compact grafting layer, favorable zwitterionic and negative-charged surface, and high magnetic susceptibility. The as-prepared Fe3 O4 @PCL-PILs nanospheres exhibit typical "zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC)" behaviors toward protein binding, and selectively adsorption of glycoprotein is achieved. The adsorption capacity of the magnetic nanospheres toward Immunoglobulin G is high up to 1136.4 mg g-1 , and the captured Immunoglobulin G could be efficiently recovered by using 0.5% NH3 H2 O (v/v) as stripping reagent, providing a recovery of 80.5%. Fe3 O4 @PCL-PILs nanospheres are then employed as sorbent for the selective isolation of Immunoglobulin G from human whole blood, obtaining high-purity Immunoglobulin G as demonstrated by polyacrylamide gel electrophoresis assays.- Published
- 2018
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