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This study was conducted to evaluate the effects of different levels of prebiotic GroBiotic®-A on growth factors, survival rate, body composition and intestinal microbiota of cultured juvenile beluga (Hous huso) in the sturgeon culture center (Samandak, Sari). Four groups of beluga sturgeon with mean weight of 40.82± 5.8 g were raised for 56 days in fiber glass tanks (20 fish to each tank) and feeding with different levels of GroBiotic®-A with concentrations of % 0, % 0.5, %1.0 and % 2.0 (Three replicates were used for each concentration). At the end of the trial, growth factors (final weight, weight gain, SGR, CF feed conversion ratio (FCR), body composition and intestinal microbiota (total viable bacteria and Lactobacillus spp. levels) were determined and compared with control group. Our results confirmed that juveniles fed on diet supplemented with 2% GroBiotic®-A had significantly higher growth factors and survival rate compared to control and 0.5% treatment (P0.05). Total viable bacteria and Lactobacillus spp. count were increased during this study and these parameters significantly higher in 2% treatment compared to other treatments (P