11 results on '"Manti, L"'
Search Results
2. Investigations of DNA damage induction and repair resulting from cellular exposure to high dose-rate pulsed proton beams.
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Renis, M., Borghesi, M., Favetta, M., Malfa, G., Manti, L., Romano, F., Schettino, G., Tomasello, B., and Cirrone, G. A. P.
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DNA damage ,DNA repair ,PROTON beams ,RADIOBIOLOGY ,IRRADIATION ,CANCER radiotherapy ,CELL proliferation - Abstract
Studies regarding the radiobiological effects of low dose radiation, microbeam irradiation services have been developed in the world and today laser acceleration of protons and heavy ions may be used in radiation therapy. The application of different facilities is essential for studying bystander effects and relating signalling phenomena in different cells or tissues. In particular the use of ion beams results advantageous in cancer radiotherapy compared to more commonly used X-rays, since the ability of ions in delivering lethal amount of doses into the target tumour avoiding or limiting damage to the contiguous healthy tissues. At the INFN-LNS in Catania, a multidisciplinary radiobiology group is strategically structured aimed to develop radiobiological research, finalised to therapeutic applications, compatible with the use of high dose laser-driven ion beams. The characteristic non-continuous dose rates with several orders of magnitude of laser-driven ion beams makes this facility very interesting in the cellular systems' response to ultra-high dose rates with non-conventional pulse time intervals cellular studies. Our group have projected to examine the effect of high dose laser-driven ion beams on two cellular types: foetal fibroblasts (normal control cells) and DU145 (prostate cancer cells), studying the modulation of some different bio-molecular parameters, in particular cell proliferation and viability, DNA damage, redox cellular status, morphological alterations of both the cytoskeleton components and some cell organelles and the possible presence of apoptotic or necrotic cell death. Our group performed preliminary experiments with high energy (60 MeV), dose rate of 10 Gy/min, doses of 1, 2, 3 Gy and LET 1 keV/μm on human foetal fibroblasts (control cells). We observed that cell viability was not influenced by the characteristics of the beam, the irradiation conditions or the analysis time. Conversely, DNA damage was present at time 0, immediately following irradiation in a dose-dependent manner. The analysis of repair capability showed that the cells irradiated with 1 and 2 Gy almost completely recovered from the damage, but not, however, 3 Gy treated cells in which DNA damage was not recovered. In addition, the results indicate the importance of the use of an appropriate control in radiobiological in vitro analysis. [ABSTRACT FROM AUTHOR]
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- 2013
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3. Delayed luminescence in a multiparameter approach to evaluation and reduction of radiobiological risks
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Grasso Rosaria 1, 3, Cammarata Francesco Paolo 2, Minafra Luigi 2, Marchese Valentina 2, Russo, Giorgio 2, Manti Lorenz0 4, Musumeci Francesco 1, Scordino Agata 1, Grasso, R., Cammarata, F.P., Minafra, L., Marchese, V.,Russo, G., Manti, L., Musumeci, F.,c, Scordino, A., Grasso, R., Cammarata F., P, Minafra, L, Marchese, V., Russo, G., Manti, L., Musumeci, F., and Scordino, A.
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Proton ,Chemistry ,Energy transfer ,ion irradiation ,Radiochemistry ,proton beam ,Dose dependence ,non-tumorigenic ,Mcf10a cell ,MCF10A ,Irradiation ,non-invasive technique ,Luminescence ,Clonogenic assay ,delayed luminescence ,Laser light - Abstract
In the framework of the research project ETHICS "Pre-clinical experimental and theoretical studies to improve treatment and protection by charged particles" funded by the National Nuclear Physics Institute, Italy, we studied the phenomenon called delayed luminescence emitted by non-tumorigenic breast epithelial MCF10A cell line after proton irradiation at different doses (0.5, 2, 6, 9 Gy). The aim is to found possible correlations between delayed luminescence and in vitro damaging induced by ion irradiation. The first results of this research show that the delayed luminescence kinetics is proton dose dependent. An interesting correlation between delayed luminescence and clonogenic potential was observed.
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- 2017
4. The Proton-Boron Reaction Increases the Radiobiological Effectiveness of Clinical Low- and High-Energy Proton Beams: Novel Experimental Evidence and Perspectives
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Pavel Bláha, Chiara Feoli, Stefano Agosteo, Marco Calvaruso, Francesco Paolo Cammarata, Roberto Catalano, Mario Ciocca, Giuseppe Antonio Pablo Cirrone, Valeria Conte, Giacomo Cuttone, Angelica Facoetti, Giusi Irma Forte, Lorenzo Giuffrida, Giuseppe Magro, Daniele Margarone, Luigi Minafra, Giada Petringa, Gaia Pucci, Valerio Ricciardi, Enrico Rosa, Giorgio Russo, Lorenzo Manti, Blaha, P., Feoli, C., Agosteo, S., Calvaruso, M., Cammarata, F. P., Catalano, R., Ciocca, M., Cirrone, G. A. P., Conte, V., Cuttone, G., Facoetti, A., Forte, G. I., Giuffrida, L., Magro, G., Margarone, D., Minafra, L., Petringa, G., Pucci, G., Ricciardi, V., Rosa, E., Russo, G., and Manti, L.
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Cancer Research ,Proton ,medicine.medical_treatment ,Sobp ,Bragg peak ,BSH ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,0302 clinical medicine ,Radioresistance ,medicine ,Irradiation ,RC254-282 ,Original Research ,protontherapy ,cancer cell killing ,Chemistry ,alpha-particle ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Proton-Boron Reaction ,Radiation therapy ,Cell killing ,chromosome aberrations ,Oncology ,030220 oncology & carcinogenesis ,Cancer research ,proton-boron (B) fusion-enhanced proton therapy (PBFEPT) ,chromosome aberration ,Beam (structure) - Abstract
Protontherapy is a rapidly expanding radiotherapy modality where accelerated proton beams are used to precisely deliver the dose to the tumor target but is generally considered ineffective against radioresistant tumors. Proton-Boron Capture Therapy (PBCT) is a novel approach aimed at enhancing proton biological effectiveness. PBCT exploits a nuclear fusion reaction between low-energy protons and 11B atoms, i.e. p+11B→ 3α (p-B), which is supposed to produce highly-DNA damaging α-particles exclusively across the tumor-conformed Spread-Out Bragg Peak (SOBP), without harming healthy tissues in the beam entrance channel. To confirm previous work on PBCT, here we report new in-vitro data obtained at the 62-MeV ocular melanoma-dedicated proton beamline of the INFN-Laboratori Nazionali del Sud (LNS), Catania, Italy. For the first time, we also tested PBCT at the 250-MeV proton beamline used for deep-seated cancers at the Centro Nazionale di Adroterapia Oncologica (CNAO), Pavia, Italy. We used Sodium Mercaptododecaborate (BSH) as 11B carrier, DU145 prostate cancer cells to assess cell killing and non-cancer epithelial breast MCF-10A cells for quantifying chromosome aberrations (CAs) by FISH painting and DNA repair pathway protein expression by western blotting. Cells were exposed at various depths along the two clinical SOBPs. Compared to exposure in the absence of boron, proton irradiation in the presence of BSH significantly reduced DU145 clonogenic survival and increased both frequency and complexity of CAs in MCF-10A cells at the mid- and distal SOBP positions, but not at the beam entrance. BSH-mediated enhancement of DNA damage response was also found at mid-SOBP. These results corroborate PBCT as a strategy to render protontherapy amenable towards radiotherapy-resilient tumor. If coupled with emerging proton FLASH radiotherapy modalities, PBCT could thus widen the protontherapy therapeutic index.
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- 2021
5. Investigations of DNA damage induction and repair resulting from cellular exposure to high dose-rate pulsed proton beams
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Manti, L. [Physics Science Department, University of Naples Federico II, Naples, and National Institute for Nuclear Physics (INFN), Naples (Italy)]
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- 2013
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6. Different approaches for Raman spectra multivariate analysis for monitoring x-rays exposed human neuroblastoma cells
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Vito Capozzi, Maria Lasalvia, Ines Delfino, Lorenzo Manti, Valerio Ricciardi, Maria Lepore, Giuseppe Perna, Popp, Jürgen, Delfino, Ine, Ricciardi, Valerio, Perna, Giuseppe, Lasalvia, Maria, Manti, Lorenzo, Capozzi, Vito, Lepore, Maria, Delfino, I., Ricciardi, V., Perna, G., Lasalvia, M., Manti, L., Capozzi, V., and Lepore, M.
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Cytoplasm ,PCA ,Chemistry ,Cell ,Spectra ,X-ray irradiation ,Interval PCA ,Ionizing radiation ,symbols.namesake ,medicine.anatomical_structure ,Raman spectroscopy ,Nucleic acid ,symbols ,Biophysics ,medicine ,Nucleu ,Irradiation ,Nucleus ,Protein secondary structure - Abstract
Raman micro-spectroscopy and different approaches for multivariate analysis were used for an investigation of subcellular regions of X-ray exposed single SH-SY5Y human neuroblastoma cells. Nucleus and cytoplasm regions of single cells were investigated after X-rays irradiation (0, 2, 4, 6 and 8 Gy). Cells fixed immediately after irradiation and 24h irradiation were considered. Principal component analysis (PCA) and interval-PCA (i-PCA) were used for analyzing the spectra in order to highlight the changes due to the different treatments. Biochemical changes occurring in the nucleus and cytoplasm regions of single cells upon X-ray irradiation were observed. The analysis of Raman spectra allowed us to detect modifications in the contribution from proteins, nucleic acids, lipids, and carbohydrates of cells, induced at different extent on the two cell regions. The biochemical changes occurring in these cells were also discussed by using an alternative approach, namely the analysis of difference spectra, obtained by subtracting the cytoplasm-related spectrum from the corresponding one detected at the nucleus. The proposed approach enabled us to evidence some features not outlined in previous investigations. The results showed that it is possible to study in a selective way the effects of ionizing radiation on different neuroblastoma cell spatial regions. An increase of the signal related to the nucleobases, a decrease of DNA and/or RNA backbone contribution, a protein rearrangement with changes in the secondary structure, and an increase in lipid saturation were observed. These results indicated that the development of accurate data analysis methods enabling to take into account the complexity of the Raman spectra of cells and tissues and the high number of spectra needed to consider the intrinsic variability of biological samples.
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- 2020
7. Raman spectroscopy for the evaluation of the radiobiological sensitivity of normal human breast cells at different time points after irradiation by a clinical proton beam
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Maria Lasalvia 1, 2, Giuseppe Perna 1, Pietro Pisciotta 3, 4, Francesco Paolo Cammarata 4, Lorenzo Manti 5, 6, Vito Capozzi 1, Lasalvia, M., Perna, G., Pisciotta, P., Cammarata, F. P., Manti, L., and Capozzi, V.
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Time Factors ,Proton ,Time Factor ,medicine.medical_treatment ,Apoptosis ,02 engineering and technology ,Spectrum Analysis, Raman ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,symbols.namesake ,Necrosis ,Breast cancer ,Electrochemistry ,medicine ,Environmental Chemistry ,Humans ,Irradiation ,Breast ,Clonogenic assay ,Proton therapy ,Spectroscopy ,Cells, Cultured ,Cellular Senescence ,Micronuclei, Chromosome-Defective ,Cell Proliferation ,Chemistry ,Raman spectroscopy ,breast cells ,010401 analytical chemistry ,Apoptosi ,Dose-Response Relationship, Radiation ,021001 nanoscience & nanotechnology ,medicine.disease ,Necrosi ,In vitro ,0104 chemical sciences ,Radiation therapy ,symbols ,Biophysics ,Female ,Protons ,0210 nano-technology ,Human ,DNA Damage - Abstract
Among different radiotherapy techniques, proton irradiation is an established and effective method for treatment of several types of cancer, because less healthy tissue is exposed with respect to conventional radiotherapy by photons/electrons. Recently, proton therapy has been proposed for the treatment of breast cancer. In vitro studies of proton irradiated normal human breast cells can provide information about cellular radioresponse, particularly as far as healthy tissue is concerned. In this paper, a study of the effects at different time points, following proton irradiation at different doses, of human normal MCF10A breast cells is performed by Raman spectroscopy. The aim of this investigation is to detect the unwanted effects of proton treatment and to investigate the possibility of monitoring them and of making an assessment of the cellular sensitivity by means of such a technique. The obtained results seem to indicate a rather significant sensitivity of MCF10A cells to proton irradiation. In fact, even at doses as low as 0.5 Gy, biological effects are clearly detectable in Raman spectra. In particular, ratiometric analysis of the Raman spectra measured from the nucleoplasm compartment showed that DNA/RNA damage increases with time, suggesting that most cells are unable to repair DNA/RNA broken bonds. The results obtained by the Raman spectroscopy analysis exhibit a similar trend with regard to dose to those obtained by commonly used radiobiological assays (i.e. MTT, clonogenic assay, senescence, apoptosis and necrosis). The results of this study strongly suggest the possibility that the Raman technique can be used to identify molecular markers predicting radiation response.
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- 2019
8. X-ray irradiation effects on nuclear and membrane regions of single SH-SY5Y human neuroblastoma cells investigated by Raman micro-spectroscopy
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Lorenzo Manti, Vito Capozzi, Valerio Ricciardi, Maria Lasalvia, Ines Delfino, Maria Lepore, Giuseppe Perna, Delfino, I., Perna, G., Ricciardi, V., Lasalvia, M., Manti, L., Capozzi, V., and Lepore, M.
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SH-SY5Y ,X-ray effects on DNA, lipids, proteins and carbohydrate ,Clinical Biochemistry ,Population ,Pharmaceutical Science ,Apoptosis ,X-Ray Therapy ,Radiation Dosage ,Spectrum Analysis, Raman ,01 natural sciences ,Analytical Chemistry ,symbols.namesake ,Neuroblastoma ,Cell Line, Tumor ,Drug Discovery ,medicine ,Humans ,Cellular nucleus and membrane ,Irradiation ,education ,Cell Nucleu ,Spectroscopy ,Cell Nucleus ,education.field_of_study ,010405 organic chemistry ,Chemistry ,Drug Discovery3003 Pharmaceutical Science ,Multivariate analysi ,010401 analytical chemistry ,Cell Cycle ,Cell Membrane ,Apoptosi ,Dose-Response Relationship, Radiation ,Cell cycle ,0104 chemical sciences ,Single SH-SY5Y human cancer cell ,Cell nucleus ,medicine.anatomical_structure ,Membrane ,Single-Cell Analysi ,Raman micro-spectroscopy ,Biophysics ,symbols ,Single-Cell Analysis ,Raman spectroscopy ,Nucleus ,Human - Abstract
Raman micro-spectroscopy was performed in vitro on nuclear and membrane regions of single SH-SY5Y human neuroblastoma cells after irradiation by graded X-ray doses (2, 4, 6, 8 Gy). The acquired spectra were analyzed by principal component analysis (PCA) and interval-PCA (i-PCA) methods. Biochemical changes occurring in the different regions of single cells as a consequence of the radiation exposure were observed in cells fixed immediately after the irradiation. The most relevant effects arose from the analysis of the spectra from the cell nucleus region. The observed changes were discussed in terms of the modifications in the cell cycle, resulting in an increase in the DNA-related signal, a protein rearrangement and changes in lipid and carbohydrates profiles within the nucleus. Potential markers of an apoptotic process in cell population irradiated with 6 and 8-Gy X-ray doses could have been singled out. No significant effects were found in spectra from cells fixed 24 h after the irradiation, thus suggesting the occurrence of repairing processes of the X-ray induced damage.
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- 2019
9. Raman spectroscopy monitoring of MCF10A cells irradiated by protons at clinical doses
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Maria Lasalvia, Javier Rasero, Vito Capozzi, Lorenzo Manti, Giuseppe Perna, Sebastiano Stramaglia, Lasalvia, M., Perna, G., Manti, L., Rasero, J., Stramaglia, S., and Capozzi, V.
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Materials science ,Radiobiology ,Proton ,medicine.medical_treatment ,proton beam ,Spectrum Analysis, Raman ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,symbols.namesake ,0302 clinical medicine ,Breast cancer ,Nuclear magnetic resonance ,medicine ,Proton Therapy ,Humans ,Radiology, Nuclear Medicine and imaging ,Irradiation ,Radiosensitivity ,Breast ,Proton therapy ,Cells, Cultured ,Radiological and Ultrasound Technology ,medicine.disease ,Radiation therapy ,030220 oncology & carcinogenesis ,Raman spectroscopy ,symbols ,Female ,DNA Damage - Abstract
Purpose Proton therapy has been recently proposed as a radiotherapy form for breast cancer treatment in view of its potentially decreased normal-tissue toxicity compared with conventional photon-based radiotherapy. However, the risks for the healthy tissue cannot be completely eliminated. In the present study, the suitability of Raman spectroscopy to monitor the radiosensitivity of normal cells exposed to clinical proton beam was investigated. Materials and methods MCF10A normal human breast cells were irradiated at two different proton doses: 0.5 Gy and 4 Gy. They were fixed immediately after irradiation and measured by means of Raman spectroscopy technique. The obtained data were analyzed both by evaluating the intensity ratio of specific Raman spectral peaks and through Multivariate Distance Matrix Regression technique. Results Certain Raman peaks associated with DNA showed a systematic suppression at both dose levels. In particular, the intensity of a Raman peak at 784 cm-1, related to a stretching mode inside the phosphate group of DNA, is very sensitive to the proton beam exposure, even at the lowest investigated dose. Therefore, it could be considered as a spectral marker of cytogenetic damage. Conclusions The obtained results are encouraging for the future of Raman spectroscopy in radiobiology research, particularly for improving risk assessment in the field of proton radiotherapy. Specifically, these findings validate Raman spectroscopy to measure biological response in human breast cells exposed to standard proton therapy doses used in clinical setting.
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- 2019
10. Multivariate analysis of difference Raman spectra of the irradiated nucleus and cytoplasm region of SH-SY5Y human neuroblastoma cells
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Lorenzo Manti, Maria Lasalvia, Maria Lepore, Valerio Ricciardi, Ines Delfino, Delfino, Ine, Ricciardi, Valerio, Manti, Lorenzo, Lasalvia, Maria, Lepore, Maria, Delfino, I., Ricciardi, V., Manti, L., Lasalvia, M., and Lepore, M.
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SH-SY5Y ,Cell ,lcsh:Chemical technology ,Spectrum Analysis, Raman ,01 natural sciences ,Biochemistry ,Article ,Analytical Chemistry ,010309 optics ,symbols.namesake ,chemistry.chemical_compound ,Neuroblastoma ,Principal Component Analysi ,Cell Line, Tumor ,0103 physical sciences ,medicine ,Humans ,lcsh:TP1-1185 ,Irradiation ,Electrical and Electronic Engineering ,Instrumentation ,Cell Nucleus ,Principal Component Analysis ,Chemistry ,X-Rays ,010401 analytical chemistry ,RNA ,single SH-SY5Y human cancer cells ,Atomic and Molecular Physics, and Optics ,0104 chemical sciences ,medicine.anatomical_structure ,Cytoplasm ,Child, Preschool ,Raman micro-spectroscopy ,Multivariate Analysis ,Biophysics ,symbols ,Female ,effects of X-rays on cell nucleus and cytoplasm ,Raman spectroscopy ,Nucleus ,DNA - Abstract
Previous works showed that spatially resolved Raman spectra of cytoplasm and nucleus region of single cells exposed to X-rays evidence different features. The present work aims to introduce a new approach to profit from these differences to deeper investigate X-ray irradiation effects on single SH-SY5Y human neuroblastoma cells. For this aim, Raman micro-spectroscopy was performed in vitro on single cells after irradiation by graded X-ray doses (2, 4, 6, 8 Gy). Spectra from nucleus and cytoplasm regions were selectively acquired. The examination by interval Principal Component Analysis (i-PCA) of the difference spectra obtained by subtracting each cytoplasm-related spectrum from the corresponding one detected at the nucleus enabled us to reveal the subtle modifications of Raman features specific of different spatial cell regions. They were discussed in terms of effects induced by X-ray irradiation on DNA/RNA, lipids, and proteins. The proposed approach enabled us to evidence some features not outlined in previous investigations.
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- 2019
11. Interval-Principal Component Analysis of Raman spectra of single cells exposed to X-ray radiation
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Carlo Camerlingo, Giuseppe Perna, Lorenzo Manti, Maria Lepore, Vito Capozzi, Ines Delfino, IEEE, 345 E 47TH ST, NEW YORK, NY 10017 USA, Delfino, I, Camerlingo, C, Capozzi, V, Perna, G, Manti, Lorenzo, Lepore, M., I. Delfino, C. Camerlingo, G. Perna, V. Capozzi, M. Lepore, Delfino, I., Capozzi, V., Perna, G., Camerlingo, C., Manti, L., and Lepore, Maria
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X-ray induced biological damage ,Chemistry ,medicine.medical_treatment ,Analytical chemistry ,X-ray ,Micro-Raman spectroscopy ,Radiation ,Ionizing radiation ,Radiation therapy ,symbols.namesake ,Principal component analysis ,medicine ,Biophysics ,symbols ,Irradiation ,Spectroscopy ,Raman spectroscopy - Abstract
A Micro-Raman spectroscopy investigation has been performed on human mammary epithelial cells after irradiation by different X-ray doses. The analysis by interval Principal Component Analysis (iPCA) has allowed us to outline the subtle differences in the Raman spectra induced by irradiation. In particular, this experimental approach has enabled to delineate radiation-induced changes in structure, protein, nucleic acid, lipid, and carbohydrate content. This has further confirmed that micro-Raman spectroscopy coupled to properly chosen data analysis methods is a very sensitive technique to detect molecular changes even in single human cells following exposure to ionising radiation. This would help developing innovative approaches to monitor radiation treatment effects so as to reduce the overall radiation dose and minimize damage to the surrounding healthy cells, both aspects being of great importance in the field of radiation therapy. Keywords-Micro-Raman spectroscopy; X-ray induced damage cells; interval principal component analaysis
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- 2014
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