1. Assay of tyrosol and hydroxytyrosol in olive oil by tandem mass spectrometry and isotope dilution method
- Author
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Mazzotti, Fabio, Benabdelkamel, Hicham, Di Donna, Leonardo, Maiuolo, Loredana, Napoli, Anna, and Sindona, Giovanni
- Subjects
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OLIVE oil , *TYROSOL , *HYDROXYTYROSOL , *TANDEM mass spectrometry , *ISOTOPE dilution analysis , *FOOD chemistry - Abstract
Abstract: Hydroxytyrosol and tyrosol, the strong antioxidant present in large amount in virgin olive oil have been assayed by LC–MS/MS under MRM condition and isotope dilution method, using d 2-labelled internal standards obtained by simple synthetic procedures. The assay has been performed under MRM condition monitoring two transitions for each analyte to improve the specificity. This paper deals with a modern approach for assaying the content of this polyphenols in virgin olive oil down to a limit of a few hundreds of parts per billion. Tyrosol and hydroxytyrosol ranged from 10 to 47ppm and from 5 to 25ppm in commercial olive oil, respectively. The accuracy (98–107%) and analytical parameters values confirm the reliability of the proposed approach. The method can be extended to any natural matrices, including mill wastes, after a simple step of sample preparation. [Copyright &y& Elsevier]
- Published
- 2012
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