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There are various mosquitos borne diseases playing major role of transmission as vector; these include dengue, malaria, filariasis and yellow fever. Various places around us everywhere act as breeding sites for different developmental stages of mosquitoes. Variety of chemicals are being used to control breeding and spread of mosquitoes which include mercuric chloride, phenols, Paris green cresols, naphthalene, Bordeaux mixture, calcium arsenate, rosin-fish oil soap, and nicotine sulfate which are prone to various risks on environment and biodiversity. In present study an alternative approach of control through insectivore fish Poecilia reticulata and Poecilia sphenops was used against mosquitoe's larvae as an alternative approach. The predation effect of insectivore fish on mosquito larvae (200) of culex and aedes was observed at different temperatures including 20°C, 25°C and 30°C for one hour duration and observation was recorded at 5 minutes interval. At 20°C the maximum consumption of larvae was observed at 35 minutes while for 25°C the maximum consumption was observed at 55 minutes similar results are observed at 30°C with increase of time for Poecilia reticulate. Poecilia sphenops also showed significant results at recorded temperatures. Also the morphometric parameters of mosquito's larvae are shown in results which gave significant results. This study gave a useful alternate for control of breeding sites of mosquitoes which can save mankind from various serious mosquitoes' related diseases. [ABSTRACT FROM AUTHOR]

Current study was designed to evaluate in vivo and in vitro anthelmintic activity of Artemisia vestita Wall ex DC. and Artemisia maritima L. against Haemonchus contortus in comparison with ivermectin to investigate the effect of plant extracts on survival of infective L 3 and adults under in vitro condition. Plant extracts were given to H. contortus infected sheep orally and it was infected with L 3 stage of H. contortus at dose of 5000 larvae/sheep. Total of 25–30 larvae were incubated with plant extracts in PBS alone and ivermectin at different concentration used as positive control. It was recorded that there is a significant decrease in fecal egg count (FEC) after post-treatment period for both plants. The highest fecal egg count reduction for A. vestita was 87.2% at 100 mg/kg while for A. maritima it was 84.5% on day 28 post-treatment. Investigated extracts indicated significant activity against larvae and adult worms. [ABSTRACT FROM AUTHOR]