1. Terminal keratinocyte differentiation in vitro is associated with a stable DNA methylome.
- Author
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Smits JPH, Dirks RAM, Qu J, Oortveld MAW, Brinkman AB, Zeeuwen PLJM, Schalkwijk J, Zhou H, Marks H, and van den Bogaard EH
- Subjects
- Adult, DNA-Binding Proteins genetics, Humans, Transcription Factors genetics, Cell Differentiation genetics, DNA Methylation genetics, Epigenome genetics, Keratinocytes metabolism
- Abstract
The epidermal compartment of the skin is regenerated constantly by proliferation of epidermal keratinocytes. Differentiation of a subset of these keratinocytes allows the epidermis to retain its barrier properties. Regulation of keratinocyte fate-whether to remain proliferative or terminally differentiate-is complex and not fully understood. The objective of our study was to assess if DNA methylation changes contribute to the regulation of keratinocyte fate. We employed genome-wide MethylationEPIC beadchip array measuring approximately 850 000 probes combined with RNA sequencing of in vitro cultured non-differentiated and terminally differentiated adult human primary keratinocytes. We did not observe a correlation between methylation status and transcriptome changes. Moreover, only two differentially methylated probes were detected, of which one was located in the TRIM29 gene. Although TRIM29 knock-down resulted in lower expression levels of terminal differentiation genes, these changes were minor. From these results, we conclude that-in our in vitro experimental setup-it is unlikely that changes in DNA methylation have an important regulatory role in terminal keratinocyte differentiation., (© 2020 The Authors. Experimental Dermatology published by John Wiley & Sons Ltd.)
- Published
- 2021
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