1. Trophoblasts regulate natural killer cells via control of interleukin-15 receptor signaling.
- Author
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Park, Seo Y., Yun, Sohyun, Ryu, Byung J., Han, Ae R., and Lee, Sung K.
- Subjects
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KILLER cells , *INTERLEUKIN-15 , *CELL receptors , *TROPHOBLAST , *PHOSPHORYLATION - Abstract
Problem Trophoblasts are known to decrease natural killer ( NK) cell cytotoxicity. However, little is known about the interaction between trophoblasts and NK cells during pregnancy. Interleukin-15 ( IL-15) is essential for priming NK cells and maximizing their effector functions. We investigated whether trophoblasts regulate NK cell activation via IL-15/ IL-2 receptor and its signaling pathways. Method of study Natural killer-92 cells were primed with human first-trimester trophoblast cells (Sw.71) conditioned medium ( CM) and co-cultured with K562 cells. Flow cytometry, Western blot analysis, and real-time PCR were performed to assess NK cell cytotoxicity, IL-15/ IL-2 receptor expression, phosphorylation of STAT5 and MAPKs, and mRNA expression of IL-15-related genes. Results Natural killer-92 cells incubated with Sw.71 CM showed reduced cytotoxicity and IL-15-mediated proliferation, and expression of IL-15/ IL-2 receptor subunits. STAT5 phosphorylation, EOMES and T-bet mRNA expressions, and ERK/ JNK pathways of NK 92 cells were suppressed by Sw.71 CM. Productions of perforin, granzyme B, and IFN-γ were also downregulated. Conclusion Trophoblasts regulate human NK cell functions via suppression of IL-15/ IL-2 receptor expression, transcription factors, and ERK/ JNK pathways. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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