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Start Over Author "Sano, Tyler" Topic lab-on-chip
2 results on '"Sano, Tyler"'

1. All-in-One Optofluidic Chip for Molecular Biosensing Assays

Author

Sano, Tyler, Zhang, Han, Losakul, Ravipa, and Schmidt, Holger

Subjects
Analytical Chemistry, Biochemistry and Cell Biology, Chemical Sciences, Biological Sciences, Bioengineering, 4.1 Discovery and preclinical testing of markers and technologies, Detection, screening and diagnosis, Biosensing Techniques, Humans, Lab-On-A-Chip Devices, Lasers, Microspheres, Zika Virus, Zika Virus Infection, optofluidics, biosensor, lab-on-chip, PDMS, Biochemistry and cell biology, Analytical chemistry
Abstract

Integrated biosensor platforms have become subjects of high interest for consolidated assay preparation and analysis to reduce sample-to-answer response times. By compactly combining as many biosensor processes and functions as possible into a single lab-on-chip device, all-in-one point-of-care devices can aid in the accessibility and speed of deployment due to their compact size and portability. Biomarker assay preparation and sensing are functionalities that are often carried out on separate devices, thus increasing opportunity of contamination, loss of sample volume, and other forms of error. Here, we demonstrate a complete lab-on-chip system combining sample preparation, on-chip optofluidic dye laser, and optical detection. We first show the integration of an on-chip distributed feedback dye laser for alignment-free optical excitation of particles moving through a fluidic channel. This capability is demonstrated by using Rhodamine 6G as the gain medium to excite single fluorescent microspheres at 575 nm. Next, we present an optofluidic PDMS platform combining a microvalve network (automaton) for sample preparation of nanoliter volumes, on-chip distributed feedback dye laser for target excitation, and optical detection. We conduct concurrent capture and fluorescence tagging of Zika virus nucleic acid on magnetic beads in 30 min. Target-carrying beads are then optically excited using the on-chip laser as they flow through an analysis channel, followed by highly specific fluorescence detection. This demonstration of a complete all-in-one biosensor is a tangible step in the development of a rapid, point-of-care device that can assist in limiting the severity of future outbreaks.

Published
2022

2. Integrated Optofluidic PDMS Biosensor Devices

Author

Sano, Tyler Taira

Subjects
Electrical engineering, Lab-on-Chip, Microfluidics, On-Chip Lasers, Optofluidics
Abstract

Recent epidemics such as Zika and SARS-CoV-2 have demonstrated the vital need for novel sample-to-answer biosensors that enable rapid diagnoses to reduce impacts of future disease outbreaks. Additionally, it is essential that these new biosensors remain cost-effective and progress ultimately towards a complete point-of-care (POC) device, enabling access to high quality diagnostic options in developing countries where disease outbreaks are far more devastating due to lack of resources and aid. Optofluidics provides unique advantages to address this challenge. By combining the integration of photonic components with the ability to control nanoliter scales of fluids, multiple functionalities that would usually require an entire laboratory can exist in the same chip. Developing these devices establishes a pathway to point-of-care devices that can democratize infectious disease screening. By fabricating these devices on polydimethylsiloxane (PDMS), even more advantages can be exploited through rapid prototyping of novel devices. Furthermore, the advancement of organ-on-chip studies provides another avenue by which optofluidic biosensor devices can be used to investigate secreted biomarkers and inform on organ health and development. Within this thesis several PDMS-based optofluidic devices are presented. The first device demonstrated an on-chip distributed feedback laser using Rhodamine 6G dissolved in ethylene glycol that had a central lasing wavelength of 574.6 nm and a bandwidth of 1.08 nm. The threshold fluence of this laser was determined to be 52.7 µJ/cm2. A second DFB laser using 4-(dicyanomethylene)-2-methyl-6-(4-dimethylaminostyryl)-4H-pyran (DCM) dissolved in dimethyl sulfoxide (DMSO) was then added to demonstrate spatially multiplexed fluorescence sensing. This second laser had a lasing wavelength of 656.5 nm, a FWHM of 1.73 nm, and threshold fluence of 307.6 µJ/cm2. A complete all-in-one lab-on-chip (LOC) system is then presented integrating the Rhodamine 6G laser with sample preparation and optical detection of Zika nucleic acids. Lastly, new directions for optofluidic biosensors are presented including educational outreach using remotely operated LOC devices to conduct fluorescence detection of Escherichia coli (E. coli) as well as optical detection of single extracellular vesicles secreted from cerebral organoids.

Published
2023

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